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The map label for this gene is comE [H]

Identifier: 32266614

GI number: 32266614

Start: 1085054

End: 1086655

Strand: Direct

Name: comE [H]

Synonym: HH1115

Alternate gene names: 32266614

Gene position: 1085054-1086655 (Clockwise)

Preceding gene: 32266613

Following gene: 32266615

Centisome position: 60.31

GC content: 35.27

Gene sequence:

>1602_bases
ATGAAAAGGCAAAATGCGCTTTTTATCTTATGTGTTTATCTCGTCCCACTTATTGATTTTGCGTGTGATATACCCATTAA
TATTCGTTCAGGCAGTAACGCCCCTTTAAGTAAAATCATTGAAGAAATTGCTACACAATGCCACCTTAACGTCATATATC
TCCAAGAAAATACCAAAACTATGCTTGATAACAAAAAAACATCTATTCATATTGCAAACAAATCTCTTAAAAATGTCCTT
GATAGTCTTATTATAGGCAATGATTTTCACTACACCCTGCAAAAAGACACGCTTAAAATTGGTTTTTTACTCACTAAAAC
CTTTGAAATTAGCTATATCGCTACTACTCGCGTAGGTTCAAGCAATACCGATATTGTATTCTCACAAGATAACCAAGTTC
AATCTCTTTACAATACTACAAACTCTGCGCCTCTTGCATTTTCAAATTTTGAAATGCAATCCCAAGCGCATAAAATGGCA
ATGGACAAAGCCACCTCAAGCAATCCCAACATTGGCAAAAGTGGCACAAAAATTTATAGTATTGATGAAATTGATTTTTG
GGGCGAGTTGCAAAATGAAATCGCAGGTGTAGCTTATGCTCCCGGTGATATATATCAGCCACAAATGTCGGAATTAAAAG
GAAAAAACAAAGATATTATCATCAATAAAGCTGCTGGTTTGCTTACTATTACGGCTTCTCCATTACAAATAAAACGAGTT
GAGCGATATATAGCGCAGCTTAATGACAAGATTCAAAAACAAGTGCTTATTGATGTACATATTCTTAATGTTCAACACAC
AGATAACCAAACTTATGGTATTGATTGGAATGAGTTTTATAAACTTGGCAATATTATGACACTTCCCGCAGAGCATCAAA
ATACAAGTAGCGGAAGCATAGATTCTAGTGGAGGCAGATTTGCGATTAATATATTTTCACAAGGCGTAAATATTAATCGT
ATTGTAGAGTTTTTGCAAACCTATGGCAAAGTCCAATCTATCTCTAACCCAAAAGTCCTTACCCTCAATAATCAACCTGC
CATTATTAGCGTAGGAAGTGTGCTTCGTTATTCACAAAACACCACTTATCAAACTACTACACAAGGCACTTCGGTGCAAA
ATAACTCTCAAGCCTTTCCCTCTGTCTTTGCTGGAATTCTCCTTGATGTAACACCAAGTATTAAAGATGGCAAGATTATT
CTTAAAATCAACCCTTCCATTACGAAAACAAAAGATATTTCTATTGAGAATCAAACAATTGCTCTAAATGCACCACCTAA
TCTTTCCACCAAACAACTTTCCTCACTTGTGCAGCTTAAAGATGGTGAGCAAATCGTGCTTGGTGGCTTAATTGATAAAA
CAGAAGGACAAACTACTCGTAAAATTCCTATTCTTGGGGATATTCCTTTTGTCAAATATTTCTTTAGCTATAAGCAAAAT
ATCAAAGAAACTCAAGAAATGGTTATTATCATAGCACCCCATATCGTGCGATTAGATGCTAACGAAACTGCCGCTAGTCC
AAAAGCAGAGCATATTCAAAAGATGAATCTTATAGATAATAAAGACATACAAGATTCTCAAAATGCACCAAAGGAACAAT
GA

Upstream 100 bases:

>100_bases
GCTATCTCATTGTCAAAATAGAATCTCAATCTGTCATTTTGCAAAAAGCAAAAGCGCAATTTAACCTTGCAATAGGCGAA
AAAATAACAAAAGGAAACCA

Downstream 100 bases:

>100_bases
TGCAGCCCAAAGTATATGCTCAATTCTCGCCTGTGAATCTCCTTGAACATACGCTTTGTTCTTGGTGTTTAAATGCCCTT
GAATATAAATTTATCCTCAA

Product: hypothetical protein

Products: NA

Alternate protein names: DNA transformation protein ComE [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MKRQNALFILCVYLVPLIDFACDIPINIRSGSNAPLSKIIEEIATQCHLNVIYLQENTKTMLDNKKTSIHIANKSLKNVL
DSLIIGNDFHYTLQKDTLKIGFLLTKTFEISYIATTRVGSSNTDIVFSQDNQVQSLYNTTNSAPLAFSNFEMQSQAHKMA
MDKATSSNPNIGKSGTKIYSIDEIDFWGELQNEIAGVAYAPGDIYQPQMSELKGKNKDIIINKAAGLLTITASPLQIKRV
ERYIAQLNDKIQKQVLIDVHILNVQHTDNQTYGIDWNEFYKLGNIMTLPAEHQNTSSGSIDSSGGRFAINIFSQGVNINR
IVEFLQTYGKVQSISNPKVLTLNNQPAIISVGSVLRYSQNTTYQTTTQGTSVQNNSQAFPSVFAGILLDVTPSIKDGKII
LKINPSITKTKDISIENQTIALNAPPNLSTKQLSSLVQLKDGEQIVLGGLIDKTEGQTTRKIPILGDIPFVKYFFSYKQN
IKETQEMVIIIAPHIVRLDANETAASPKAEHIQKMNLIDNKDIQDSQNAPKEQ

Sequences:

>Translated_533_residues
MKRQNALFILCVYLVPLIDFACDIPINIRSGSNAPLSKIIEEIATQCHLNVIYLQENTKTMLDNKKTSIHIANKSLKNVL
DSLIIGNDFHYTLQKDTLKIGFLLTKTFEISYIATTRVGSSNTDIVFSQDNQVQSLYNTTNSAPLAFSNFEMQSQAHKMA
MDKATSSNPNIGKSGTKIYSIDEIDFWGELQNEIAGVAYAPGDIYQPQMSELKGKNKDIIINKAAGLLTITASPLQIKRV
ERYIAQLNDKIQKQVLIDVHILNVQHTDNQTYGIDWNEFYKLGNIMTLPAEHQNTSSGSIDSSGGRFAINIFSQGVNINR
IVEFLQTYGKVQSISNPKVLTLNNQPAIISVGSVLRYSQNTTYQTTTQGTSVQNNSQAFPSVFAGILLDVTPSIKDGKII
LKINPSITKTKDISIENQTIALNAPPNLSTKQLSSLVQLKDGEQIVLGGLIDKTEGQTTRKIPILGDIPFVKYFFSYKQN
IKETQEMVIIIAPHIVRLDANETAASPKAEHIQKMNLIDNKDIQDSQNAPKEQ
>Mature_533_residues
MKRQNALFILCVYLVPLIDFACDIPINIRSGSNAPLSKIIEEIATQCHLNVIYLQENTKTMLDNKKTSIHIANKSLKNVL
DSLIIGNDFHYTLQKDTLKIGFLLTKTFEISYIATTRVGSSNTDIVFSQDNQVQSLYNTTNSAPLAFSNFEMQSQAHKMA
MDKATSSNPNIGKSGTKIYSIDEIDFWGELQNEIAGVAYAPGDIYQPQMSELKGKNKDIIINKAAGLLTITASPLQIKRV
ERYIAQLNDKIQKQVLIDVHILNVQHTDNQTYGIDWNEFYKLGNIMTLPAEHQNTSSGSIDSSGGRFAINIFSQGVNINR
IVEFLQTYGKVQSISNPKVLTLNNQPAIISVGSVLRYSQNTTYQTTTQGTSVQNNSQAFPSVFAGILLDVTPSIKDGKII
LKINPSITKTKDISIENQTIALNAPPNLSTKQLSSLVQLKDGEQIVLGGLIDKTEGQTTRKIPILGDIPFVKYFFSYKQN
IKETQEMVIIIAPHIVRLDANETAASPKAEHIQKMNLIDNKDIQDSQNAPKEQ

Specific function: Involved in transformation (competence for DNA uptake) [H]

COG id: COG1450

COG function: function code NU; Type II secretory pathway, component PulD

Gene ontology:

Cell location: Cell outer membrane (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP D family [H]

Homologues:

Organism=Escherichia coli, GI87082242, Length=290, Percent_Identity=25.8620689655172, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1789793, Length=270, Percent_Identity=23.3333333333333, Blast_Score=72, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001775
- InterPro:   IPR005644
- InterPro:   IPR013355
- InterPro:   IPR004846
- InterPro:   IPR004845
- InterPro:   IPR001814 [H]

Pfam domain/function: PF00263 Secretin; PF03958 Secretin_N [H]

EC number: NA

Molecular weight: Translated: 59171; Mature: 59171

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRQNALFILCVYLVPLIDFACDIPINIRSGSNAPLSKIIEEIATQCHLNVIYLQENTKT
CCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHEEEEEEEECCCHH
MLDNKKTSIHIANKSLKNVLDSLIIGNDFHYTLQKDTLKIGFLLTKTFEISYIATTRVGS
HHCCCCCEEEEECHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEEEEEEEEEEEEECCC
SNTDIVFSQDNQVQSLYNTTNSAPLAFSNFEMQSQAHKMAMDKATSSNPNIGKSGTKIYS
CCCCEEEECCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
IDEIDFWGELQNEIAGVAYAPGDIYQPQMSELKGKNKDIIINKAAGLLTITASPLQIKRV
ECCCCHHHHHHHHHCCEEECCCCCCCCHHHHHCCCCCEEEEEECCCEEEEECCCHHHHHH
ERYIAQLNDKIQKQVLIDVHILNVQHTDNQTYGIDWNEFYKLGNIMTLPAEHQNTSSGSI
HHHHHHHHHHHHHEEEEEEEEEEEEECCCCEECCCHHHHHHCCCEEECCCCCCCCCCCCC
DSSGGRFAINIFSQGVNINRIVEFLQTYGKVQSISNPKVLTLNNQPAIISVGSVLRYSQN
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCHHHEECCC
TTYQTTTQGTSVQNNSQAFPSVFAGILLDVTPSIKDGKIILKINPSITKTKDISIENQTI
CCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEECCCEE
ALNAPPNLSTKQLSSLVQLKDGEQIVLGGLIDKTEGQTTRKIPILGDIPFVKYFFSYKQN
EEECCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEEEEECCHHHHHHHHHHHH
IKETQEMVIIIAPHIVRLDANETAASPKAEHIQKMNLIDNKDIQDSQNAPKEQ
HHHHHEEEEEEECEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKRQNALFILCVYLVPLIDFACDIPINIRSGSNAPLSKIIEEIATQCHLNVIYLQENTKT
CCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHEEEEEEEECCCHH
MLDNKKTSIHIANKSLKNVLDSLIIGNDFHYTLQKDTLKIGFLLTKTFEISYIATTRVGS
HHCCCCCEEEEECHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEEEEEEEEEEEEEECCC
SNTDIVFSQDNQVQSLYNTTNSAPLAFSNFEMQSQAHKMAMDKATSSNPNIGKSGTKIYS
CCCCEEEECCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
IDEIDFWGELQNEIAGVAYAPGDIYQPQMSELKGKNKDIIINKAAGLLTITASPLQIKRV
ECCCCHHHHHHHHHCCEEECCCCCCCCHHHHHCCCCCEEEEEECCCEEEEECCCHHHHHH
ERYIAQLNDKIQKQVLIDVHILNVQHTDNQTYGIDWNEFYKLGNIMTLPAEHQNTSSGSI
HHHHHHHHHHHHHEEEEEEEEEEEEECCCCEECCCHHHHHHCCCEEECCCCCCCCCCCCC
DSSGGRFAINIFSQGVNINRIVEFLQTYGKVQSISNPKVLTLNNQPAIISVGSVLRYSQN
CCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEECCHHHEECCC
TTYQTTTQGTSVQNNSQAFPSVFAGILLDVTPSIKDGKIILKINPSITKTKDISIENQTI
CCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCEEEEEECCCEE
ALNAPPNLSTKQLSSLVQLKDGEQIVLGGLIDKTEGQTTRKIPILGDIPFVKYFFSYKQN
EEECCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCEEEEEEEECCHHHHHHHHHHHH
IKETQEMVIIIAPHIVRLDANETAASPKAEHIQKMNLIDNKDIQDSQNAPKEQ
HHHHHEEEEEEECEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1916268; 7542800; 1856167 [H]