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The map label for this gene is hsdM [H]

Identifier: 32266590

GI number: 32266590

Start: 1061331

End: 1063022

Strand: Direct

Name: hsdM [H]

Synonym: HH1091

Alternate gene names: 32266590

Gene position: 1061331-1063022 (Clockwise)

Preceding gene: 32266589

Following gene: 32266591

Centisome position: 58.99

GC content: 36.23

Gene sequence:

>1692_bases
ATGCAAGGACACAGCCAAGCAATTAAAGAATTGCTGAACTGCTTTGAATACATTAAACGTTATCACACAGACATACTTGA
TTCCATAGCTATTATGCTTGAGATGTTTTGGCTTAAGCGGCATTCTCCTGCATTTATTCAATCCCTCATTGCTCTTGCTG
CCAAACGAAAGCCTATTTATGAAGAATGCACAAACGCGTTAAAAGATGCGCTCGGAGAGGAGCTTTATATACCGCTTAAC
CCTCATTCTAATGTATTGAAGATTCTCAAAGTCATTATGGCAAATGATACTTCAAATCATACAATAGAGGAATTTTTGCA
TATTATCACGCAAAAAAAGACAACGCATAAGCTGTTTTCTTACTCTACCCCACTTGAAATTAATGAGCTTCTTGTAGGAT
TGCTTGATATTAAAGAATCTCAAAGCATATATAATCCGTGCTATGGCACAGGAAGCCTATTTTTTGCGATTGCTAATTAT
GCACATAGTTTTGAGCTTTATGGCGAAGAGTTAGAATCAAGCCTAGCACGGATTGCTAAAATTATTTGCAAGATTTTAGA
TTTAAATACGCAACATTTGATTTTAAATAATATTCTCAAAAATGCTCAATTTAAAAATCAAAAATTTGATAAAATCATCT
GTAATCCACCACTTGATAGTCATATAGGCACACAATTTTTAAAAGAGGACGAGCGATTTGCCACTTATGAAGCACTTATT
AAAACGTATCCTGAACTCCTTTTCTTAATTCATTCGCTCTCGCATCTTAAAGATAAAGGTGTATTTATCCTGCGCACACA
AACACTACTCAAATCCTCACTTGAAGGCAGACTAAGAGAAAAACTTTGCGAAGAAGGCTTGATAGAATCTATTATTGAAT
TGCCAAAAAATATTTTTCCTCATCAAACACACGAATTTTCAATTATCGTGCTCTCTCCAAATAATCGTGCAATTTTACAC
ATTAATGCAAATGCACCACATTTTTATCATAAAGATGGCAAATATAACCGCTTAGTTAATCTTAAAGAGCTGCTTAACAT
TTATAGACATAAATATGTTGGCACATATTCCTCCCTCACGCCTTTAAGTGAAATTGACCCACACGATTTGCGCGTTGCGC
GTTATATCCAAGAACCCCAATCTAGCAGAAAAAATCATACGCTACTGGGCGCACTCAATATCAATGTATTTCGTGGGCAA
CGCGTGTATGGAAGTGCAAAAGATGAGAAAATTACTTATTTTGATTTGGGCATTGCTGATTTTATGGATTTTGGCTTTTC
TGATGAGCTTTCCACCCAACGACTTAAGGGCGATAAAAGCAAGATTGAAAAATATCAGCTTAAACCTTATGACATTGCCT
TATCTATTCGTGGCACGACACCTAAATTCACTATTTTAAGCCCAAAGCTTAAAAATAATAAGGTAGTAGCAAATGCAGGC
ATTATCATTATGCGCTCTCCTACCCCAGAAATTGCCATAGGACTTTATTGTTACCTTTTCTCAACGCAAGGGCAAAAAGC
ACTTGGCAAGATTTATGAGCGTAATCTTGGTAGCATTAATCCACCTGATTTAGAGCAACTCGCTATCCCCGATGATTTTT
GCGCTAATGCACAAGAAACTTTCAGGCAGATTCAAAGTTATGCAAAAGAACTGAAAGTGCTTCAATCTAAACTCCAAGCA
TTAAGGGGCTAA

Upstream 100 bases:

>100_bases
CTAAGGTAATGAAAAGCCATATGGACGAACTTAACGCAGAAGCCATTACCAAACTTGCAGTGCGCGCTTAAAATTGATTC
ATTAATAATATGGAGTATTT

Downstream 100 bases:

>100_bases
ATGCAAGAACTGAAAGTGCTTCAATCAAGATTTACAAATATCACTTATGGGAAAAATGTTACATTAGTGCAGCCAAGTAA
TCTTTATGGTTGCACACTTG

Product: type I restriction enzyme

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 563; Mature: 563

Protein sequence:

>563_residues
MQGHSQAIKELLNCFEYIKRYHTDILDSIAIMLEMFWLKRHSPAFIQSLIALAAKRKPIYEECTNALKDALGEELYIPLN
PHSNVLKILKVIMANDTSNHTIEEFLHIITQKKTTHKLFSYSTPLEINELLVGLLDIKESQSIYNPCYGTGSLFFAIANY
AHSFELYGEELESSLARIAKIICKILDLNTQHLILNNILKNAQFKNQKFDKIICNPPLDSHIGTQFLKEDERFATYEALI
KTYPELLFLIHSLSHLKDKGVFILRTQTLLKSSLEGRLREKLCEEGLIESIIELPKNIFPHQTHEFSIIVLSPNNRAILH
INANAPHFYHKDGKYNRLVNLKELLNIYRHKYVGTYSSLTPLSEIDPHDLRVARYIQEPQSSRKNHTLLGALNINVFRGQ
RVYGSAKDEKITYFDLGIADFMDFGFSDELSTQRLKGDKSKIEKYQLKPYDIALSIRGTTPKFTILSPKLKNNKVVANAG
IIIMRSPTPEIAIGLYCYLFSTQGQKALGKIYERNLGSINPPDLEQLAIPDDFCANAQETFRQIQSYAKELKVLQSKLQA
LRG

Sequences:

>Translated_563_residues
MQGHSQAIKELLNCFEYIKRYHTDILDSIAIMLEMFWLKRHSPAFIQSLIALAAKRKPIYEECTNALKDALGEELYIPLN
PHSNVLKILKVIMANDTSNHTIEEFLHIITQKKTTHKLFSYSTPLEINELLVGLLDIKESQSIYNPCYGTGSLFFAIANY
AHSFELYGEELESSLARIAKIICKILDLNTQHLILNNILKNAQFKNQKFDKIICNPPLDSHIGTQFLKEDERFATYEALI
KTYPELLFLIHSLSHLKDKGVFILRTQTLLKSSLEGRLREKLCEEGLIESIIELPKNIFPHQTHEFSIIVLSPNNRAILH
INANAPHFYHKDGKYNRLVNLKELLNIYRHKYVGTYSSLTPLSEIDPHDLRVARYIQEPQSSRKNHTLLGALNINVFRGQ
RVYGSAKDEKITYFDLGIADFMDFGFSDELSTQRLKGDKSKIEKYQLKPYDIALSIRGTTPKFTILSPKLKNNKVVANAG
IIIMRSPTPEIAIGLYCYLFSTQGQKALGKIYERNLGSINPPDLEQLAIPDDFCANAQETFRQIQSYAKELKVLQSKLQA
LRG
>Mature_563_residues
MQGHSQAIKELLNCFEYIKRYHTDILDSIAIMLEMFWLKRHSPAFIQSLIALAAKRKPIYEECTNALKDALGEELYIPLN
PHSNVLKILKVIMANDTSNHTIEEFLHIITQKKTTHKLFSYSTPLEINELLVGLLDIKESQSIYNPCYGTGSLFFAIANY
AHSFELYGEELESSLARIAKIICKILDLNTQHLILNNILKNAQFKNQKFDKIICNPPLDSHIGTQFLKEDERFATYEALI
KTYPELLFLIHSLSHLKDKGVFILRTQTLLKSSLEGRLREKLCEEGLIESIIELPKNIFPHQTHEFSIIVLSPNNRAILH
INANAPHFYHKDGKYNRLVNLKELLNIYRHKYVGTYSSLTPLSEIDPHDLRVARYIQEPQSSRKNHTLLGALNINVFRGQ
RVYGSAKDEKITYFDLGIADFMDFGFSDELSTQRLKGDKSKIEKYQLKPYDIALSIRGTTPKFTILSPKLKNNKVVANAG
IIIMRSPTPEIAIGLYCYLFSTQGQKALGKIYERNLGSINPPDLEQLAIPDDFCANAQETFRQIQSYAKELKVLQSKLQA
LRG

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 64403; Mature: 64403

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGHSQAIKELLNCFEYIKRYHTDILDSIAIMLEMFWLKRHSPAFIQSLIALAAKRKPIY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHH
EECTNALKDALGEELYIPLNPHSNVLKILKVIMANDTSNHTIEEFLHIITQKKTTHKLFS
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHC
YSTPLEINELLVGLLDIKESQSIYNPCYGTGSLFFAIANYAHSFELYGEELESSLARIAK
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IICKILDLNTQHLILNNILKNAQFKNQKFDKIICNPPLDSHIGTQFLKEDERFATYEALI
HHHHHHCCCCHHHHHHHHHHHCHHCCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHH
KTYPELLFLIHSLSHLKDKGVFILRTQTLLKSSLEGRLREKLCEEGLIESIIELPKNIFP
HHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HQTHEFSIIVLSPNNRAILHINANAPHFYHKDGKYNRLVNLKELLNIYRHKYVGTYSSLT
CCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCC
PLSEIDPHDLRVARYIQEPQSSRKNHTLLGALNINVFRGQRVYGSAKDEKITYFDLGIAD
CCCCCCCHHHHHHHHHHCHHHCCCCCEEEEEEEEEEEECCEEECCCCCCEEEEEECCHHH
FMDFGFSDELSTQRLKGDKSKIEKYQLKPYDIALSIRGTTPKFTILSPKLKNNKVVANAG
HHHCCCCCCHHHHHHCCCHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCEEEEECC
IIIMRSPTPEIAIGLYCYLFSTQGQKALGKIYERNLGSINPPDLEQLAIPDDFCANAQET
EEEEECCCCCHHHHHEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHCCHHHH
FRQIQSYAKELKVLQSKLQALRG
HHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQGHSQAIKELLNCFEYIKRYHTDILDSIAIMLEMFWLKRHSPAFIQSLIALAAKRKPIY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHH
EECTNALKDALGEELYIPLNPHSNVLKILKVIMANDTSNHTIEEFLHIITQKKTTHKLFS
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHC
YSTPLEINELLVGLLDIKESQSIYNPCYGTGSLFFAIANYAHSFELYGEELESSLARIAK
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IICKILDLNTQHLILNNILKNAQFKNQKFDKIICNPPLDSHIGTQFLKEDERFATYEALI
HHHHHHCCCCHHHHHHHHHHHCHHCCCCCCEEECCCCCCHHHHHHHHHCCHHHHHHHHHH
KTYPELLFLIHSLSHLKDKGVFILRTQTLLKSSLEGRLREKLCEEGLIESIIELPKNIFP
HHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
HQTHEFSIIVLSPNNRAILHINANAPHFYHKDGKYNRLVNLKELLNIYRHKYVGTYSSLT
CCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCCCCC
PLSEIDPHDLRVARYIQEPQSSRKNHTLLGALNINVFRGQRVYGSAKDEKITYFDLGIAD
CCCCCCCHHHHHHHHHHCHHHCCCCCEEEEEEEEEEEECCEEECCCCCCEEEEEECCHHH
FMDFGFSDELSTQRLKGDKSKIEKYQLKPYDIALSIRGTTPKFTILSPKLKNNKVVANAG
HHHCCCCCCHHHHHHCCCHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCEEEEECC
IIIMRSPTPEIAIGLYCYLFSTQGQKALGKIYERNLGSINPPDLEQLAIPDDFCANAQET
EEEEECCCCCHHHHHEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHCCHHHH
FRQIQSYAKELKVLQSKLQALRG
HHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]