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The map label for this gene is glyS

Identifier: 32266566

GI number: 32266566

Start: 1039241

End: 1041352

Strand: Direct

Name: glyS

Synonym: HH1067

Alternate gene names: 32266566

Gene position: 1039241-1041352 (Clockwise)

Preceding gene: 32266564

Following gene: 32266567

Centisome position: 57.76

GC content: 37.12

Gene sequence:

>2112_bases
ATGATACAATCATATCTAAATTTTAAAGGAATATCCTTGAAATCATCATATTTGCCCTTACTTATTGAAATTTTCACTGA
AGAACTCCCCGCTCTCCCTTTTCTCAAAGAGTTACCGCATATGGTAGAAAAATGGCAAAAAATTGCGCAAAAACACCGCA
TTACTTCTACGCCCACTCTCTATTTCACACCGCGCCGCATTGTGCTTTATGAGCAAAACTTCGCCACTCATACACAAGAT
ATTCTTATAGAATCTTATGGTCCTCCCCTTGCTATTGCTTTTATTAATGGTGATAAAACAAAGGGTCTAAGCAAAGCGGG
TGAAAGCTTCTTCAAAAAACTTGAGTTGCCAACAAACACGCCTTATGAAATAAGAATCAAAGAGAATAAAGAAGTCCTCT
ATTATGCCAAAATGCAAAAAGGTGTGCAAACAAAAGATATTGTAGGAGAGATACTCATAGAGTGGCTCAAGGCATTGCAA
TTTGGCAAAAGTATGTTTTGGGGCAACTTAAATGAAAGCTTTATCCGCCCTGTGCGCAATATTTTTATCTTACTTGGAGA
ACAAAGTGTTTCTTTTAATGCTTTTGGGCTGCATAGCATTGCCCAAACTTATCCACACCGAGATATAAGCTTTGAGCCTT
ATGCCATTACCTCTCCTTCTGATTATTTTCAATCCTTAGAAAAAAGCAAAGTCATACTTGAACAAAACAAACGAAGAGCA
ATGATTTTAGAGCAAATTGTAGTCATAGAATCGCAAGAAAACATAAAGGTAGAAATTGATGAAGCATTGCTTAATGAAAT
TGTAGCCATTACAGAATATCCACGCGCAGCGTATGGAATCTTTGATAAGCAGTTCTTACAGCTACCCAATGAGGTCATTA
TTACTTCAATGAAAGAAAATCAACGATATTTTGCCACCTACAAAGACGGGCATCTCCACAATGGTTTTGTGCTTGTTGCT
AATTCTACTGCTGCTAATCTTGGACCTATTGTGCAGGGCAATCAAAAGGTTTTAAAAGCGCGCCTCTCTGATGCTGTATT
TTTCTATGAAAATGACCTTAAAAATGGATTTGCTCCTGAAAAATTAGGGCAGATTCTATTTGTTGATGGGCTAGGTTCAT
TACTTGATAAAACTAAGCGCGAAAGCGTCATTGCACTTGAATTGCTCAAATTTTATACTCATAAGATAGATATGAAGATT
CAAGAATCCCAAGAAATATTAAGCACTGCAGTGAAGTATGCAAAGGCTGATTTACTCACAGAAATGGTATATGAATTTAC
TGAACTGCAGGGAATTATAGGCTATTATTATGCGCAAAGCTTTGGTATGCACCCACTTGTTGCTCAAGCTATCAAAGAAC
AATATCTTCCCACAGGGGAAGATTCTCCACTTCCTAGCCATATACTTAGCGCAATCCTCGCCCTTAGTATCAAGCTTGAT
AACATTTTTGCTCTCTTTAGCATTGATAAGATTCCTAGCGGCTCAAAAGACCCTTTTGCTCTGCGCAGGGCTGCAAATGG
GGTCATTAAAATTATATGGCATTATGGACTAGATTTTGACTTGAGCATTGATATACCTCGCTTATATCAAGCAGGAGGCT
ATAAACCAAGCAATATGCAGCGCATTGAATCTTTTTTTCTTGAACGACTTGAGGGTATGCTCAAGGTTAATCCCTCTCTT
ATCCGATGTGTGCTTAATGCTCGTGTCAATAACGCAAAAGTGCGTAATCTCAACACCATTATACACAACATTGAAGCACT
AAGCGCATTTTTTGAAAAAAGTGACAAACAATCTCTCATTACGCTTTTTAAACGCGTAGCAAATATTCTTAACGATGATA
TAATACCCACACACATTGACGAATCTCTCTTAAGTTTTCCTCAAGAAATTGCCCTTTACCAAGCTCTTAAAGCTCTTAAA
GCAAAGGAATTTAAGGACATAAGTGAGCATATTGCTACACTTTTTTCACTCAAAGAACCCTTAGAAGCATTTTTTGCTTC
TGTGCTTGTAAATGATAATGATAATGCCATTAAGACAAATCGTCAAATGCTTATTTTGAGCATATATAATGAATTTCTCC
GCATTGGGGATATTAAGGACATTACACTTTGA

Upstream 100 bases:

>100_bases
ATTATACATAATCCAATGATGATTTTCACTCTCTAACCTCCAATAAGACTTTAGTGATTCTCAATATATGTAAATAAGTT
TTTAACAAGTCTGCATTATA

Downstream 100 bases:

>100_bases
AATCATTGTATCAAATCTTTATACTTGAATCAAAAAATATATTTACCTCTGTTTCAGCTATGCTCATTATTTTTGGGGGC
TCGCTTTTTTATCTTTTCCT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 703; Mature: 703

Protein sequence:

>703_residues
MIQSYLNFKGISLKSSYLPLLIEIFTEELPALPFLKELPHMVEKWQKIAQKHRITSTPTLYFTPRRIVLYEQNFATHTQD
ILIESYGPPLAIAFINGDKTKGLSKAGESFFKKLELPTNTPYEIRIKENKEVLYYAKMQKGVQTKDIVGEILIEWLKALQ
FGKSMFWGNLNESFIRPVRNIFILLGEQSVSFNAFGLHSIAQTYPHRDISFEPYAITSPSDYFQSLEKSKVILEQNKRRA
MILEQIVVIESQENIKVEIDEALLNEIVAITEYPRAAYGIFDKQFLQLPNEVIITSMKENQRYFATYKDGHLHNGFVLVA
NSTAANLGPIVQGNQKVLKARLSDAVFFYENDLKNGFAPEKLGQILFVDGLGSLLDKTKRESVIALELLKFYTHKIDMKI
QESQEILSTAVKYAKADLLTEMVYEFTELQGIIGYYYAQSFGMHPLVAQAIKEQYLPTGEDSPLPSHILSAILALSIKLD
NIFALFSIDKIPSGSKDPFALRRAANGVIKIIWHYGLDFDLSIDIPRLYQAGGYKPSNMQRIESFFLERLEGMLKVNPSL
IRCVLNARVNNAKVRNLNTIIHNIEALSAFFEKSDKQSLITLFKRVANILNDDIIPTHIDESLLSFPQEIALYQALKALK
AKEFKDISEHIATLFSLKEPLEAFFASVLVNDNDNAIKTNRQMLILSIYNEFLRIGDIKDITL

Sequences:

>Translated_703_residues
MIQSYLNFKGISLKSSYLPLLIEIFTEELPALPFLKELPHMVEKWQKIAQKHRITSTPTLYFTPRRIVLYEQNFATHTQD
ILIESYGPPLAIAFINGDKTKGLSKAGESFFKKLELPTNTPYEIRIKENKEVLYYAKMQKGVQTKDIVGEILIEWLKALQ
FGKSMFWGNLNESFIRPVRNIFILLGEQSVSFNAFGLHSIAQTYPHRDISFEPYAITSPSDYFQSLEKSKVILEQNKRRA
MILEQIVVIESQENIKVEIDEALLNEIVAITEYPRAAYGIFDKQFLQLPNEVIITSMKENQRYFATYKDGHLHNGFVLVA
NSTAANLGPIVQGNQKVLKARLSDAVFFYENDLKNGFAPEKLGQILFVDGLGSLLDKTKRESVIALELLKFYTHKIDMKI
QESQEILSTAVKYAKADLLTEMVYEFTELQGIIGYYYAQSFGMHPLVAQAIKEQYLPTGEDSPLPSHILSAILALSIKLD
NIFALFSIDKIPSGSKDPFALRRAANGVIKIIWHYGLDFDLSIDIPRLYQAGGYKPSNMQRIESFFLERLEGMLKVNPSL
IRCVLNARVNNAKVRNLNTIIHNIEALSAFFEKSDKQSLITLFKRVANILNDDIIPTHIDESLLSFPQEIALYQALKALK
AKEFKDISEHIATLFSLKEPLEAFFASVLVNDNDNAIKTNRQMLILSIYNEFLRIGDIKDITL
>Mature_703_residues
MIQSYLNFKGISLKSSYLPLLIEIFTEELPALPFLKELPHMVEKWQKIAQKHRITSTPTLYFTPRRIVLYEQNFATHTQD
ILIESYGPPLAIAFINGDKTKGLSKAGESFFKKLELPTNTPYEIRIKENKEVLYYAKMQKGVQTKDIVGEILIEWLKALQ
FGKSMFWGNLNESFIRPVRNIFILLGEQSVSFNAFGLHSIAQTYPHRDISFEPYAITSPSDYFQSLEKSKVILEQNKRRA
MILEQIVVIESQENIKVEIDEALLNEIVAITEYPRAAYGIFDKQFLQLPNEVIITSMKENQRYFATYKDGHLHNGFVLVA
NSTAANLGPIVQGNQKVLKARLSDAVFFYENDLKNGFAPEKLGQILFVDGLGSLLDKTKRESVIALELLKFYTHKIDMKI
QESQEILSTAVKYAKADLLTEMVYEFTELQGIIGYYYAQSFGMHPLVAQAIKEQYLPTGEDSPLPSHILSAILALSIKLD
NIFALFSIDKIPSGSKDPFALRRAANGVIKIIWHYGLDFDLSIDIPRLYQAGGYKPSNMQRIESFFLERLEGMLKVNPSL
IRCVLNARVNNAKVRNLNTIIHNIEALSAFFEKSDKQSLITLFKRVANILNDDIIPTHIDESLLSFPQEIALYQALKALK
AKEFKDISEHIATLFSLKEPLEAFFASVLVNDNDNAIKTNRQMLILSIYNEFLRIGDIKDITL

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=705, Percent_Identity=30.6382978723404, Blast_Score=277, Evalue=2e-75,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 80128; Mature: 80128

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQSYLNFKGISLKSSYLPLLIEIFTEELPALPFLKELPHMVEKWQKIAQKHRITSTPTL
CCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEE
YFTPRRIVLYEQNFATHTQDILIESYGPPLAIAFINGDKTKGLSKAGESFFKKLELPTNT
EECCCEEEEEECCCCCCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
PYEIRIKENKEVLYYAKMQKGVQTKDIVGEILIEWLKALQFGKSMFWGNLNESFIRPVRN
CEEEEEECCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHH
IFILLGEQSVSFNAFGLHSIAQTYPHRDISFEPYAITSPSDYFQSLEKSKVILEQNKRRA
HEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCHHH
MILEQIVVIESQENIKVEIDEALLNEIVAITEYPRAAYGIFDKQFLQLPNEVIITSMKEN
HHHHHHHHEECCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHEEEEECCC
QRYFATYKDGHLHNGFVLVANSTAANLGPIVQGNQKVLKARLSDAVFFYENDLKNGFAPE
CEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCCCCCHH
KLGQILFVDGLGSLLDKTKRESVIALELLKFYTHKIDMKIQESQEILSTAVKYAKADLLT
HHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHH
EMVYEFTELQGIIGYYYAQSFGMHPLVAQAIKEQYLPTGEDSPLPSHILSAILALSIKLD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
NIFALFSIDKIPSGSKDPFALRRAANGVIKIIWHYGLDFDLSIDIPRLYQAGGYKPSNMQ
CEEEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCEEEEECCHHHHHCCCCCCCHHH
RIESFFLERLEGMLKVNPSLIRCVLNARVNNAKVRNLNTIIHNIEALSAFFEKSDKQSLI
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
TLFKRVANILNDDIIPTHIDESLLSFPQEIALYQALKALKAKEFKDISEHIATLFSLKEP
HHHHHHHHHHCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAFFASVLVNDNDNAIKTNRQMLILSIYNEFLRIGDIKDITL
HHHHHHHHHCCCCCCCEECCCCEEEHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MIQSYLNFKGISLKSSYLPLLIEIFTEELPALPFLKELPHMVEKWQKIAQKHRITSTPTL
CCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEE
YFTPRRIVLYEQNFATHTQDILIESYGPPLAIAFINGDKTKGLSKAGESFFKKLELPTNT
EECCCEEEEEECCCCCCHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCC
PYEIRIKENKEVLYYAKMQKGVQTKDIVGEILIEWLKALQFGKSMFWGNLNESFIRPVRN
CEEEEEECCCCEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHH
IFILLGEQSVSFNAFGLHSIAQTYPHRDISFEPYAITSPSDYFQSLEKSKVILEQNKRRA
HEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHCCCHHH
MILEQIVVIESQENIKVEIDEALLNEIVAITEYPRAAYGIFDKQFLQLPNEVIITSMKEN
HHHHHHHHEECCCCCEEEEHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHEEEEECCC
QRYFATYKDGHLHNGFVLVANSTAANLGPIVQGNQKVLKARLSDAVFFYENDLKNGFAPE
CEEEEEECCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCCCCCHH
KLGQILFVDGLGSLLDKTKRESVIALELLKFYTHKIDMKIQESQEILSTAVKYAKADLLT
HHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHHH
EMVYEFTELQGIIGYYYAQSFGMHPLVAQAIKEQYLPTGEDSPLPSHILSAILALSIKLD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
NIFALFSIDKIPSGSKDPFALRRAANGVIKIIWHYGLDFDLSIDIPRLYQAGGYKPSNMQ
CEEEEEEECCCCCCCCCHHHHHHHCCCCEEEEECCCCCEEEEECCHHHHHCCCCCCCHHH
RIESFFLERLEGMLKVNPSLIRCVLNARVNNAKVRNLNTIIHNIEALSAFFEKSDKQSLI
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
TLFKRVANILNDDIIPTHIDESLLSFPQEIALYQALKALKAKEFKDISEHIATLFSLKEP
HHHHHHHHHHCCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEAFFASVLVNDNDNAIKTNRQMLILSIYNEFLRIGDIKDITL
HHHHHHHHHCCCCCCCEECCCCEEEHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA