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The map label for this gene is ogt

Identifier: 32266536

GI number: 32266536

Start: 1015879

End: 1016391

Strand: Direct

Name: ogt

Synonym: HH1037

Alternate gene names: 32266536

Gene position: 1015879-1016391 (Clockwise)

Preceding gene: 32266535

Following gene: 32266537

Centisome position: 56.46

GC content: 47.37

Gene sequence:

>513_bases
ATGATTTATACGCAGCAATTCCACTCACCGCTTGGCACAATCGCACTTGCAAGTGATAAAAGCACACTACTTGGCTTATG
GTTTGAGGGGCAAAAGTATTTTACATTGCCAAAAGATTGTGTGCGGGAGCAAACGCCGATTTTAGCCCAAACGCAACAAT
GGCTAGAGATTTACTTCAGCGGAGAGATTCCACATTTCACTCCGCCTCTAGCTCCACTTCACACGCAAAGCACACCCTTT
AGAGAATCTGTATGGACAATCTTACGCACAATCCCCTATGGACGCACGATAACTTACAAGGAGATTGCACAAACTCTCGC
TTGCCAAAGAGGCATAGCCAAAATGTCCGCTCAAGCTGTGGGCGGTGCAGTAGGAGCAAATCCTATCGCGCTACTTATCC
CTTGCCACCGAGTGATTGGGAGTGATAAAAGCCTCACGGGTTATGCGGGAGGTTTGAAACGCAAAGAGTGGCTATTAGGG
CTTGAGAGGGAATCCCTAAAGGCTATCAAGTAA

Upstream 100 bases:

>100_bases
CAACAAAATGGACAAAAAGTAGAAATTATAGAAGACGGGGATAACGGAGCTTAGGAGCAAAATCGTTTCATCATTACCTT
TGATTATATGTGGAGTTTTA

Downstream 100 bases:

>100_bases
CGCGCAATTTAGGTAATTAATGCTATAATAGGCATTTTATGGAGGCGCAAAGGAAATGGGGAATATCGCTGCCAAACTTT
CGTCTTTGTCTCAACTACAA

Product: methylated DNA-protein cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 170; Mature: 170

Protein sequence:

>170_residues
MIYTQQFHSPLGTIALASDKSTLLGLWFEGQKYFTLPKDCVREQTPILAQTQQWLEIYFSGEIPHFTPPLAPLHTQSTPF
RESVWTILRTIPYGRTITYKEIAQTLACQRGIAKMSAQAVGGAVGANPIALLIPCHRVIGSDKSLTGYAGGLKRKEWLLG
LERESLKAIK

Sequences:

>Translated_170_residues
MIYTQQFHSPLGTIALASDKSTLLGLWFEGQKYFTLPKDCVREQTPILAQTQQWLEIYFSGEIPHFTPPLAPLHTQSTPF
RESVWTILRTIPYGRTITYKEIAQTLACQRGIAKMSAQAVGGAVGANPIALLIPCHRVIGSDKSLTGYAGGLKRKEWLLG
LERESLKAIK
>Mature_170_residues
MIYTQQFHSPLGTIALASDKSTLLGLWFEGQKYFTLPKDCVREQTPILAQTQQWLEIYFSGEIPHFTPPLAPLHTQSTPF
RESVWTILRTIPYGRTITYKEIAQTLACQRGIAKMSAQAVGGAVGANPIALLIPCHRVIGSDKSLTGYAGGLKRKEWLLG
LERESLKAIK

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=172, Percent_Identity=34.8837209302326, Blast_Score=81, Evalue=4e-16,
Organism=Escherichia coli, GI1787596, Length=105, Percent_Identity=45.7142857142857, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1788542, Length=152, Percent_Identity=33.5526315789474, Blast_Score=83, Evalue=8e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=88, Percent_Identity=40.9090909090909, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18900; Mature: 18900

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYTQQFHSPLGTIALASDKSTLLGLWFEGQKYFTLPKDCVREQTPILAQTQQWLEIYFS
CCCCHHHCCCCCEEEEECCCCEEEEEEECCCEEEECCHHHHHCCCCHHHHHHHHHEEEEC
GEIPHFTPPLAPLHTQSTPFRESVWTILRTIPYGRTITYKEIAQTLACQRGIAKMSAQAV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
GGAVGANPIALLIPCHRVIGSDKSLTGYAGGLKRKEWLLGLERESLKAIK
CCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCC
>Mature Secondary Structure
MIYTQQFHSPLGTIALASDKSTLLGLWFEGQKYFTLPKDCVREQTPILAQTQQWLEIYFS
CCCCHHHCCCCCEEEEECCCCEEEEEEECCCEEEECCHHHHHCCCCHHHHHHHHHEEEEC
GEIPHFTPPLAPLHTQSTPFRESVWTILRTIPYGRTITYKEIAQTLACQRGIAKMSAQAV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
GGAVGANPIALLIPCHRVIGSDKSLTGYAGGLKRKEWLLGLERESLKAIK
CCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA