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The map label for this gene is glnA

Identifier: 32266059

GI number: 32266059

Start: 537878

End: 539317

Strand: Direct

Name: glnA

Synonym: HH0560

Alternate gene names: 32266059

Gene position: 537878-539317 (Clockwise)

Preceding gene: 32266058

Following gene: 32266061

Centisome position: 29.9

GC content: 40.76

Gene sequence:

>1440_bases
ATGAGTAGAGTAAGCATAGATAAACAAGCGGTAGCAGAGTTTTTTAGATTCTGTGATGAAAATGAAGTAGAATTTATTGA
TTTCCGTTTTAGTGACATTAAAGGCGTATGGCATCATTTGAGCTTTTCTCGCAATGCAATTAACGAAGAAAGCTTTGAGG
GCATACCTTTTGATGGTAGCTCTATCCCTGCTTGGCAGCCCGTAGATAAATCCGATATGATGCTTATCCCTGAACCTGTG
CGGTATTTCATTGACCCATTTACTGCTGATACCACTGCGGTAGTCTTTTGCGATATTTGGGACATTTACAACAATAAGCC
TTATGAGAAATGTCCGCGCTCCATTGTTAAACGTGCAATGAAATATCTCAAAGAAAGCGGTATTGGCGATATCGCCTACT
ATGGACCAGAGAATGAGTTTTTTGTATTTGATTCTATTAAGATTAAAGATTCTGTGAATTGTCAATATTATGAGATAGAT
TCCGAAGAGGGCGAATGGAATCGTGCGCGTGAGTTTGAAGGCGGTGTAAATATGGGGCATCGTCCCGGCACAAAAGGTGG
GTATTTTCCTGTGCCACCTGTAGATTCTATGGTGGATTTACGCGCTGAAATCGTCAAAGTGCTTAATCAAGTAGGATTAG
AAACTTTCGTGCTTCACCACGAAGTCGCACAAGGGCAAAATGAAATTGGTGTGAAATTTGGCGATATGCTTGAAGCAGCA
GATAATGTACAAAAACTCAAATATGTTGTCAAAATGGTTGCTCATCTCAATGGCAAAACCGCGACTTTTATGCCAAAGCC
ACTTTATGGAGATAATGGCAGTGGTATGCACGTGCATATTAGTATTTGGAAAGATGGGCATAATCTCTTTGCAGGAGAAG
CATACCAAGGCTTAAGCGAAATGGCTCTACATTTCTTAGGCGGTGTGCTTAAACACGCACGCGCATTAGCCGCCTTTACA
AATCCTAGCACAAACTCATATAAACGCCTTATACCGGGATTTGAAGCACCAAGTATCCTCACTTATTCCGCACAAAACCG
CTCTGCAAGTATCAGAATCCCATATAATAGCGGCGAGAAAGCAAAACGAATGGAATTTAGATTCCCAGATAGCTCTGCAA
ATCCTTATCTTGCATTTGCAAGCCTCTTACTCGCAGGAATTGATGGGATAAAAAACAAAATTGACCCGGGCGCGCCTATG
GAAATCAATCTTTTTGAACTTACTTTAGATGAAATTCGCGAAAAAGGCATTAAACAACTACCACACACTTTGCGCCACGC
TATTGAGGAAATGCTTGTTGATAAAGCATATCTTAAAGAAGGCGGTGTATTTAGTGAAGAATTTATCCAAACTTATAAAG
CATATAAGTTTGAAACAGAGATTTGGCCTTGGGAGGCACGCCCTCACCCATTTGAGTTCCTTACTACTTATAGTTGCTAG

Upstream 100 bases:

>100_bases
AAATTTATTTTAAAAACTTTGGCATTTGTTAAAGAAAGATTCACTTATGCTATAATCGCCACATTTTGATTCTATAAATT
AAAGCATTAAAGGAGTATTG

Downstream 100 bases:

>100_bases
ATGTTAAATTATGTAGTGAGGCTTAGTGTATGTGTAATCTCTGATGCTCTATTTTGATGCAGCTATTTTCCTCATACAAA
ATGCCATACTCACGTGCAAT

Product: glutamine synthase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MSRVSIDKQAVAEFFRFCDENEVEFIDFRFSDIKGVWHHLSFSRNAINEESFEGIPFDGSSIPAWQPVDKSDMMLIPEPV
RYFIDPFTADTTAVVFCDIWDIYNNKPYEKCPRSIVKRAMKYLKESGIGDIAYYGPENEFFVFDSIKIKDSVNCQYYEID
SEEGEWNRAREFEGGVNMGHRPGTKGGYFPVPPVDSMVDLRAEIVKVLNQVGLETFVLHHEVAQGQNEIGVKFGDMLEAA
DNVQKLKYVVKMVAHLNGKTATFMPKPLYGDNGSGMHVHISIWKDGHNLFAGEAYQGLSEMALHFLGGVLKHARALAAFT
NPSTNSYKRLIPGFEAPSILTYSAQNRSASIRIPYNSGEKAKRMEFRFPDSSANPYLAFASLLLAGIDGIKNKIDPGAPM
EINLFELTLDEIREKGIKQLPHTLRHAIEEMLVDKAYLKEGGVFSEEFIQTYKAYKFETEIWPWEARPHPFEFLTTYSC

Sequences:

>Translated_479_residues
MSRVSIDKQAVAEFFRFCDENEVEFIDFRFSDIKGVWHHLSFSRNAINEESFEGIPFDGSSIPAWQPVDKSDMMLIPEPV
RYFIDPFTADTTAVVFCDIWDIYNNKPYEKCPRSIVKRAMKYLKESGIGDIAYYGPENEFFVFDSIKIKDSVNCQYYEID
SEEGEWNRAREFEGGVNMGHRPGTKGGYFPVPPVDSMVDLRAEIVKVLNQVGLETFVLHHEVAQGQNEIGVKFGDMLEAA
DNVQKLKYVVKMVAHLNGKTATFMPKPLYGDNGSGMHVHISIWKDGHNLFAGEAYQGLSEMALHFLGGVLKHARALAAFT
NPSTNSYKRLIPGFEAPSILTYSAQNRSASIRIPYNSGEKAKRMEFRFPDSSANPYLAFASLLLAGIDGIKNKIDPGAPM
EINLFELTLDEIREKGIKQLPHTLRHAIEEMLVDKAYLKEGGVFSEEFIQTYKAYKFETEIWPWEARPHPFEFLTTYSC
>Mature_478_residues
SRVSIDKQAVAEFFRFCDENEVEFIDFRFSDIKGVWHHLSFSRNAINEESFEGIPFDGSSIPAWQPVDKSDMMLIPEPVR
YFIDPFTADTTAVVFCDIWDIYNNKPYEKCPRSIVKRAMKYLKESGIGDIAYYGPENEFFVFDSIKIKDSVNCQYYEIDS
EEGEWNRAREFEGGVNMGHRPGTKGGYFPVPPVDSMVDLRAEIVKVLNQVGLETFVLHHEVAQGQNEIGVKFGDMLEAAD
NVQKLKYVVKMVAHLNGKTATFMPKPLYGDNGSGMHVHISIWKDGHNLFAGEAYQGLSEMALHFLGGVLKHARALAAFTN
PSTNSYKRLIPGFEAPSILTYSAQNRSASIRIPYNSGEKAKRMEFRFPDSSANPYLAFASLLLAGIDGIKNKIDPGAPME
INLFELTLDEIREKGIKQLPHTLRHAIEEMLVDKAYLKEGGVFSEEFIQTYKAYKFETEIWPWEARPHPFEFLTTYSC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=467, Percent_Identity=52.2483940042827, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI87081870, Length=362, Percent_Identity=26.2430939226519, Blast_Score=105, Evalue=8e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 54414; Mature: 54283

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRVSIDKQAVAEFFRFCDENEVEFIDFRFSDIKGVWHHLSFSRNAINEESFEGIPFDGS
CCCCCCCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SIPAWQPVDKSDMMLIPEPVRYFIDPFTADTTAVVFCDIWDIYNNKPYEKCPRSIVKRAM
CCCCCCCCCCCCCEECCCHHHHHHCCCCCCCEEEEEEEEHHHHCCCCHHHHHHHHHHHHH
KYLKESGIGDIAYYGPENEFFVFDSIKIKDSVNCQYYEIDSEEGEWNRAREFEGGVNMGH
HHHHHCCCCCEEEECCCCCEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHCCCCCCCCC
RPGTKGGYFPVPPVDSMVDLRAEIVKVLNQVGLETFVLHHEVAQGQNEIGVKFGDMLEAA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
DNVQKLKYVVKMVAHLNGKTATFMPKPLYGDNGSGMHVHISIWKDGHNLFAGEAYQGLSE
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCEECCHHHHCHHH
MALHFLGGVLKHARALAAFTNPSTNSYKRLIPGFEAPSILTYSAQNRSASIRIPYNSGEK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCC
AKRMEFRFPDSSANPYLAFASLLLAGIDGIKNKIDPGAPMEINLFELTLDEIREKGIKQL
HHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHH
PHTLRHAIEEMLVDKAYLKEGGVFSEEFIQTYKAYKFETEIWPWEARPHPFEFLTTYSC
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCHHHEEECCC
>Mature Secondary Structure 
SRVSIDKQAVAEFFRFCDENEVEFIDFRFSDIKGVWHHLSFSRNAINEESFEGIPFDGS
CCCCCCHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
SIPAWQPVDKSDMMLIPEPVRYFIDPFTADTTAVVFCDIWDIYNNKPYEKCPRSIVKRAM
CCCCCCCCCCCCCEECCCHHHHHHCCCCCCCEEEEEEEEHHHHCCCCHHHHHHHHHHHHH
KYLKESGIGDIAYYGPENEFFVFDSIKIKDSVNCQYYEIDSEEGEWNRAREFEGGVNMGH
HHHHHCCCCCEEEECCCCCEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHCCCCCCCCC
RPGTKGGYFPVPPVDSMVDLRAEIVKVLNQVGLETFVLHHEVAQGQNEIGVKFGDMLEAA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHHHHHH
DNVQKLKYVVKMVAHLNGKTATFMPKPLYGDNGSGMHVHISIWKDGHNLFAGEAYQGLSE
HHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEEEEEEEECCCCEECCHHHHCHHH
MALHFLGGVLKHARALAAFTNPSTNSYKRLIPGFEAPSILTYSAQNRSASIRIPYNSGEK
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCEEEEECCCCCEEEEECCCCCCC
AKRMEFRFPDSSANPYLAFASLLLAGIDGIKNKIDPGAPMEINLFELTLDEIREKGIKQL
HHEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHH
PHTLRHAIEEMLVDKAYLKEGGVFSEEFIQTYKAYKFETEIWPWEARPHPFEFLTTYSC
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]