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The map label for this gene is purF

Identifier: 32265987

GI number: 32265987

Start: 473048

End: 474472

Strand: Direct

Name: purF

Synonym: HH0488

Alternate gene names: 32265987

Gene position: 473048-474472 (Clockwise)

Preceding gene: 32265986

Following gene: 32265988

Centisome position: 26.29

GC content: 38.95

Gene sequence:

>1425_bases
ATGGAAAGCTGGAACGAAGAGTGCGCCATTGTGGGCGTATATAATGTTGATAATGCAGCACTCTTAAGCTATTATTCACT
CTTTGCAATGCAGCATAGGGGGCAGGAAGCAAGTGGGATTTCCGCAAGTAATGGCACAAAAATCACAACTATCAAAAATA
CTGGTTTAGTAACTAAAATTTTTACACAAAAGCGACTTGATAAATTGCAAGGTCGCTCAAGTATTGGACACAATCGCTAT
TCAACTGCAGGTGAAGATTCTATAAATGACACACAACCTATTTTTGCACGATATGATTTAGGCGAAATGGCAATCGCACA
TAATGGGAATCTCACAAATGCCAAAGAAGTGCGCGAGATGCTTATTAAAGAGGGAACAATATTCCAAAGCTATCTTGATA
CTGAAAATCTCATTCATCTGATTGCACGAAGTAAAAAAACATCGCTTACTGATAGAATCATTGATGCTTTAAGCTACATT
GATGGAGCGTATTGCTTTGTATTTCTTTCACGGAGCAAAATGTTTGCTATTCGTGATAGGTATGGATTGCGCCCACTTAG
CTTAGGCAAAATACAAAACGAAGACGGAAGCACAGGCTATGTTGTAGCGAGTGAAAGTTGTGCATTTGACCTTATAGGTG
CGGAATTTGTCCGAGATGTCAAAGCAGGTGAGATGATTATTTTTGATGGAGCGTATGCCTTTCAAGATTCTCAACCCAAA
AGTATTCAAGTCTTTGAACCAAAACCGCATTTTTGCGTATTTGAATACATATATTTTGCACGTCCTGATAGCAATGTGTT
TGGACATAATGTCTATCAAGCACGCAAACAAATGGGTATAGAGCTTGCTAAAGAACATCAAATTCAAGCAGATATGGTAA
TTCCTGTGCCAGATTCTGGTGTGGCTGCAGCTATTGGTTATAGTAAGCAAAGCGGAATTGATTTTGAGCTAGGATTTATC
CGCAATCACTATGTAGGAAGGACATTTATTGAGCCAACGCAGCAAGAGAGAGAGCTTAAAGTTCGCCTTAAACTCAACCC
TATCAGAGAACTTATAGCTAATAAAGATATTATTGTCATTGATGATTCTGTGGTTAGAGGCACGACAAGTAAGCAAATTA
TTAAAATCTTGCGTCAAGCGGGGGCAAGAAAAATTCATCTACTTATTAGCTCTCCGCCTACGATTTCGCCGTGCTTTTAT
GGTATAGATACGCCCAATAAGGAGCAACTCATTTGTGCTAATAAAAGTATTGAAGAAGTGTGTAAGCATATCGGTGCAGA
TTCTCTAGGTTTTCTCTCCCTTAGCGGTTTGACCAAAAGTATAGGCGAAAACAATTCTTTATGCAAAGCGTGTTTTGATG
GACATTATATTGATGATTATACCACAGGTGCAGCAATCCCTGCCGCTTTAAAATCCTCTCATTAA

Upstream 100 bases:

>100_bases
CTTTATAGTATTGAGGATGCACTTGAGATTTAAGCTTTAAATTTGCTTGTTGGTTTTGTTACTATACAATCGCAGATACA
AAACATAATGAGGATAAGGA

Downstream 100 bases:

>100_bases
AGATATATAATGCGTGTAATGTTAAGTATAGGGCGATATTTTAAAAGACGTTTTGGCACAAGGGTAAGAAAGATTCCTAT
TTCTTTACAAGGATTTACTT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MESWNEECAIVGVYNVDNAALLSYYSLFAMQHRGQEASGISASNGTKITTIKNTGLVTKIFTQKRLDKLQGRSSIGHNRY
STAGEDSINDTQPIFARYDLGEMAIAHNGNLTNAKEVREMLIKEGTIFQSYLDTENLIHLIARSKKTSLTDRIIDALSYI
DGAYCFVFLSRSKMFAIRDRYGLRPLSLGKIQNEDGSTGYVVASESCAFDLIGAEFVRDVKAGEMIIFDGAYAFQDSQPK
SIQVFEPKPHFCVFEYIYFARPDSNVFGHNVYQARKQMGIELAKEHQIQADMVIPVPDSGVAAAIGYSKQSGIDFELGFI
RNHYVGRTFIEPTQQERELKVRLKLNPIRELIANKDIIVIDDSVVRGTTSKQIIKILRQAGARKIHLLISSPPTISPCFY
GIDTPNKEQLICANKSIEEVCKHIGADSLGFLSLSGLTKSIGENNSLCKACFDGHYIDDYTTGAAIPAALKSSH

Sequences:

>Translated_474_residues
MESWNEECAIVGVYNVDNAALLSYYSLFAMQHRGQEASGISASNGTKITTIKNTGLVTKIFTQKRLDKLQGRSSIGHNRY
STAGEDSINDTQPIFARYDLGEMAIAHNGNLTNAKEVREMLIKEGTIFQSYLDTENLIHLIARSKKTSLTDRIIDALSYI
DGAYCFVFLSRSKMFAIRDRYGLRPLSLGKIQNEDGSTGYVVASESCAFDLIGAEFVRDVKAGEMIIFDGAYAFQDSQPK
SIQVFEPKPHFCVFEYIYFARPDSNVFGHNVYQARKQMGIELAKEHQIQADMVIPVPDSGVAAAIGYSKQSGIDFELGFI
RNHYVGRTFIEPTQQERELKVRLKLNPIRELIANKDIIVIDDSVVRGTTSKQIIKILRQAGARKIHLLISSPPTISPCFY
GIDTPNKEQLICANKSIEEVCKHIGADSLGFLSLSGLTKSIGENNSLCKACFDGHYIDDYTTGAAIPAALKSSH
>Mature_474_residues
MESWNEECAIVGVYNVDNAALLSYYSLFAMQHRGQEASGISASNGTKITTIKNTGLVTKIFTQKRLDKLQGRSSIGHNRY
STAGEDSINDTQPIFARYDLGEMAIAHNGNLTNAKEVREMLIKEGTIFQSYLDTENLIHLIARSKKTSLTDRIIDALSYI
DGAYCFVFLSRSKMFAIRDRYGLRPLSLGKIQNEDGSTGYVVASESCAFDLIGAEFVRDVKAGEMIIFDGAYAFQDSQPK
SIQVFEPKPHFCVFEYIYFARPDSNVFGHNVYQARKQMGIELAKEHQIQADMVIPVPDSGVAAAIGYSKQSGIDFELGFI
RNHYVGRTFIEPTQQERELKVRLKLNPIRELIANKDIIVIDDSVVRGTTSKQIIKILRQAGARKIHLLISSPPTISPCFY
GIDTPNKEQLICANKSIEEVCKHIGADSLGFLSLSGLTKSIGENNSLCKACFDGHYIDDYTTGAAIPAALKSSH

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=500, Percent_Identity=34, Blast_Score=291, Evalue=8e-79,
Organism=Escherichia coli, GI1788651, Length=474, Percent_Identity=38.8185654008439, Blast_Score=291, Evalue=8e-80,
Organism=Escherichia coli, GI1790167, Length=235, Percent_Identity=25.1063829787234, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17554892, Length=480, Percent_Identity=36.0416666666667, Blast_Score=317, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6323958, Length=456, Percent_Identity=34.4298245614035, Blast_Score=246, Evalue=8e-66,
Organism=Drosophila melanogaster, GI24659604, Length=465, Percent_Identity=38.2795698924731, Blast_Score=322, Evalue=4e-88,
Organism=Drosophila melanogaster, GI28573187, Length=468, Percent_Identity=38.6752136752137, Blast_Score=322, Evalue=5e-88,
Organism=Drosophila melanogaster, GI24659598, Length=465, Percent_Identity=38.2795698924731, Blast_Score=321, Evalue=6e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52576; Mature: 52576

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESWNEECAIVGVYNVDNAALLSYYSLFAMQHRGQEASGISASNGTKITTIKNTGLVTKI
CCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHH
FTQKRLDKLQGRSSIGHNRYSTAGEDSINDTQPIFARYDLGEMAIAHNGNLTNAKEVREM
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHH
LIKEGTIFQSYLDTENLIHLIARSKKTSLTDRIIDALSYIDGAYCFVFLSRSKMFAIRDR
HHHCCCHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEEECCCEEEEHHC
YGLRPLSLGKIQNEDGSTGYVVASESCAFDLIGAEFVRDVKAGEMIIFDGAYAFQDSQPK
CCCCCEECCCEECCCCCEEEEEEECCCCEEHHHHHHHHHCCCCCEEEEECEEEECCCCCC
SIQVFEPKPHFCVFEYIYFARPDSNVFGHNVYQARKQMGIELAKEHQIQADMVIPVPDSG
EEEEECCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHCCCCEEEEEEECCCCC
VAAAIGYSKQSGIDFELGFIRNHYVGRTFIEPTQQERELKVRLKLNPIRELIANKDIIVI
CEEEECCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHCCCCEEEE
DDSVVRGTTSKQIIKILRQAGARKIHLLISSPPTISPCFYGIDTPNKEQLICANKSIEEV
CCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCEEECCCCCCCCEEEECCCCHHHH
CKHIGADSLGFLSLSGLTKSIGENNSLCKACFDGHYIDDYTTGAAIPAALKSSH
HHHCCCCCCCEEEHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure
MESWNEECAIVGVYNVDNAALLSYYSLFAMQHRGQEASGISASNGTKITTIKNTGLVTKI
CCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCHHH
FTQKRLDKLQGRSSIGHNRYSTAGEDSINDTQPIFARYDLGEMAIAHNGNLTNAKEVREM
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEEEECCCCCCCHHHHHHH
LIKEGTIFQSYLDTENLIHLIARSKKTSLTDRIIDALSYIDGAYCFVFLSRSKMFAIRDR
HHHCCCHHHHHHCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEEEEEECCCEEEEHHC
YGLRPLSLGKIQNEDGSTGYVVASESCAFDLIGAEFVRDVKAGEMIIFDGAYAFQDSQPK
CCCCCEECCCEECCCCCEEEEEEECCCCEEHHHHHHHHHCCCCCEEEEECEEEECCCCCC
SIQVFEPKPHFCVFEYIYFARPDSNVFGHNVYQARKQMGIELAKEHQIQADMVIPVPDSG
EEEEECCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHCCHHHHCCCCEEEEEEECCCCC
VAAAIGYSKQSGIDFELGFIRNHYVGRTFIEPTQQERELKVRLKLNPIRELIANKDIIVI
CEEEECCCCCCCCCEEEEEHHCCCCCCCCCCCCCCCCEEEEEEEECHHHHHHCCCCEEEE
DDSVVRGTTSKQIIKILRQAGARKIHLLISSPPTISPCFYGIDTPNKEQLICANKSIEEV
CCCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCEEECCCCCCCCEEEECCCCHHHH
CKHIGADSLGFLSLSGLTKSIGENNSLCKACFDGHYIDDYTTGAAIPAALKSSH
HHHCCCCCCCEEEHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]