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The map label for this gene is hflB [C]

Identifier: 32265973

GI number: 32265973

Start: 459280

End: 461037

Strand: Direct

Name: hflB [C]

Synonym: HH0474

Alternate gene names: 32265973

Gene position: 459280-461037 (Clockwise)

Preceding gene: 32265972

Following gene: 32265974

Centisome position: 25.53

GC content: 35.1

Gene sequence:

>1758_bases
ATGAAGCATTATCTTGATTTTATTTCGTGTAAAGATTTAACAAATAATAGCCTTTTTGGATTCCTTAAGTGTAATGAAAA
TGAAGGAAAAATTTTGCAATTTATGGCAAAAGCCTTGCTTGAGGGTGAAATGGATTTTGTCGTGCATATATTACTTGCTA
AAGTCTTCCCACACAAAGACACCTCAAGCACACAAGGTGAGCATAGCCTTGCATTTTTGCATCATATTCGTAATCTTATT
GAGTTAGGTTGGATTACTCAAAGCTCATTCTTACAGCCCAAACTTACCGATATTGCATTACTTGAGCTCTATAATGATAA
TATTTGTCTCTCACACACCTTTTTGAAACTCCTTGAAGATGGCACGCTTAATGTTGATTTGCCCGAAATCACTCCCTATG
AAGACCACCTTGAATATCTCAAAGACCGCTTTGCGCATATTGATTTACTTCATAAAATGAGTGGGCTTAAATTTTCGCAA
AAAATAGATTCTCCCTCTCTTTTACGTGCAAATTATCGCCTTAAGCTCCTTGAAGATACTATCAACTCACGCTTAAAGCT
TACAACTAAAGATATTAGTATTATCAAGCTTATCAAAGAACATCACTTAGAACAAAAAGAACAGATTATCTTTTTTGCCC
TGCTCAAAGAAGAATATTCAAGTAGTGATGAGAATTTACGCGATATAAATGCTTTACTTGAGCTCATTAGCCTTGATGAG
TATGACAAAATCAAACATCGCTCTTTACTTGATGAAAATTCTAATCTTGTGCAAAAGGGCTTAGTGGATTATGATGAATT
GCTTAATCCTTTTGGGGGCATATCACGCACATTTTTTATCCCAGAAGAAATACTTCAAGATATCATTCACCCTAACAAAA
AAAAGAAACGCGCTAAAATCAGTCTTGATTCTATTATCAAAGAGCAAGAGATTTTTGAGCTTGTGGAGCCAAAACAAAGC
ATTAATGATGTGATTTTGCACTCTCACACGCGTGAGATTTTGGACAATCTCTTAGCTCAAGTCAATAGCAATGTAATTTC
TCTGCTTAGACTTTGGGGGATAAAAGATAAGAAAAAGGGCATTGATGCAAAAATTCTTCTTTATGGTGCAGCTGGCACAG
GTAAAACCATTACTGCTCTAGCTTTGGCTAAAAGCCTTAAAAAGCAAGTGTTAAGCTTTGATTGTTCAAAGATTCTCTCT
ATGTATGTGGGAGAATCCGAAAAAAATGTGCGCAAAATTTTTGATAGCTACCAAGAAATTTGCAAAAAAATTAAAAATGA
GCCTATTTTGCTCCTTGATGAAGCTGACCAATTCCTTAGCACGCGCGCAACTAGTGGCAATAGTGCAGATAAAATGCACA
ACCAAATGCAAAATATTTTTTTAGAGCAAATTGAGCGATTTGAGGGCATTTTGATTGCAACAACCAATCTTTTAGAAAAC
TTTGATAGCGCATTCTCGCGGCGATTTAATTATAAAATTGAATTTAAACGACCAAATAAAGAGCAAAGAATCGCTCTATG
GCTTAAATATCTGCCAAAAAATGCAGATTTTGGAGAAGATTGCAAAGAAAATCTTGCCAATGCACTTGCAGAATATAACT
TAAGTGGAGGACAGATTCACCTTATTGTTAAAAATACCGCTTATAAAGTCGCCACAAAAAAAGAGCCTATTTTCACACAA
CAAGATTTTATCACACATATCAAGGCTGAGCTTAGCGGAAATTTTGATAATGCTAAGAGTATGGGCTTTAGAAGTTAA

Upstream 100 bases:

>100_bases
TTTAAATATATTAAAGGAAAATTGTGTCAAAACACACTCCTAAAAAGCATTTAATTAATAAATACTCATCAAAGGAAATA
CAGGGGAGGGGTAGTAATAA

Downstream 100 bases:

>100_bases
TTTGTTTTTTAGACTTTATTTTCTATAATACCTTAACTTCACTTACAAGGATACGATATGGATTGCTTTTTAACGGGGGG
GGGGGCATAAGCTAGACATT

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MKHYLDFISCKDLTNNSLFGFLKCNENEGKILQFMAKALLEGEMDFVVHILLAKVFPHKDTSSTQGEHSLAFLHHIRNLI
ELGWITQSSFLQPKLTDIALLELYNDNICLSHTFLKLLEDGTLNVDLPEITPYEDHLEYLKDRFAHIDLLHKMSGLKFSQ
KIDSPSLLRANYRLKLLEDTINSRLKLTTKDISIIKLIKEHHLEQKEQIIFFALLKEEYSSSDENLRDINALLELISLDE
YDKIKHRSLLDENSNLVQKGLVDYDELLNPFGGISRTFFIPEEILQDIIHPNKKKKRAKISLDSIIKEQEIFELVEPKQS
INDVILHSHTREILDNLLAQVNSNVISLLRLWGIKDKKKGIDAKILLYGAAGTGKTITALALAKSLKKQVLSFDCSKILS
MYVGESEKNVRKIFDSYQEICKKIKNEPILLLDEADQFLSTRATSGNSADKMHNQMQNIFLEQIERFEGILIATTNLLEN
FDSAFSRRFNYKIEFKRPNKEQRIALWLKYLPKNADFGEDCKENLANALAEYNLSGGQIHLIVKNTAYKVATKKEPIFTQ
QDFITHIKAELSGNFDNAKSMGFRS

Sequences:

>Translated_585_residues
MKHYLDFISCKDLTNNSLFGFLKCNENEGKILQFMAKALLEGEMDFVVHILLAKVFPHKDTSSTQGEHSLAFLHHIRNLI
ELGWITQSSFLQPKLTDIALLELYNDNICLSHTFLKLLEDGTLNVDLPEITPYEDHLEYLKDRFAHIDLLHKMSGLKFSQ
KIDSPSLLRANYRLKLLEDTINSRLKLTTKDISIIKLIKEHHLEQKEQIIFFALLKEEYSSSDENLRDINALLELISLDE
YDKIKHRSLLDENSNLVQKGLVDYDELLNPFGGISRTFFIPEEILQDIIHPNKKKKRAKISLDSIIKEQEIFELVEPKQS
INDVILHSHTREILDNLLAQVNSNVISLLRLWGIKDKKKGIDAKILLYGAAGTGKTITALALAKSLKKQVLSFDCSKILS
MYVGESEKNVRKIFDSYQEICKKIKNEPILLLDEADQFLSTRATSGNSADKMHNQMQNIFLEQIERFEGILIATTNLLEN
FDSAFSRRFNYKIEFKRPNKEQRIALWLKYLPKNADFGEDCKENLANALAEYNLSGGQIHLIVKNTAYKVATKKEPIFTQ
QDFITHIKAELSGNFDNAKSMGFRS
>Mature_585_residues
MKHYLDFISCKDLTNNSLFGFLKCNENEGKILQFMAKALLEGEMDFVVHILLAKVFPHKDTSSTQGEHSLAFLHHIRNLI
ELGWITQSSFLQPKLTDIALLELYNDNICLSHTFLKLLEDGTLNVDLPEITPYEDHLEYLKDRFAHIDLLHKMSGLKFSQ
KIDSPSLLRANYRLKLLEDTINSRLKLTTKDISIIKLIKEHHLEQKEQIIFFALLKEEYSSSDENLRDINALLELISLDE
YDKIKHRSLLDENSNLVQKGLVDYDELLNPFGGISRTFFIPEEILQDIIHPNKKKKRAKISLDSIIKEQEIFELVEPKQS
INDVILHSHTREILDNLLAQVNSNVISLLRLWGIKDKKKGIDAKILLYGAAGTGKTITALALAKSLKKQVLSFDCSKILS
MYVGESEKNVRKIFDSYQEICKKIKNEPILLLDEADQFLSTRATSGNSADKMHNQMQNIFLEQIERFEGILIATTNLLEN
FDSAFSRRFNYKIEFKRPNKEQRIALWLKYLPKNADFGEDCKENLANALAEYNLSGGQIHLIVKNTAYKVATKKEPIFTQ
QDFITHIKAELSGNFDNAKSMGFRS

Specific function: Seems To Act As An ATP-Dependent Zinc Metallopeptidase. Involved In The Degradation Of Sigma-32. Degrades Carboxy- Terminal-Tagged Cytoplasmic Proteins. These Proteins Are Tagged With An 11-Amino-Acid Nonpolar Destabilizing Tail VIa A Mechanism Involving

COG id: COG0464

COG function: function code O; ATPases of the AAA+ class

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AAA ATPase family [H]

Homologues:

Organism=Homo sapiens, GI24497435, Length=149, Percent_Identity=32.8859060402685, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI21361144, Length=276, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI226371754, Length=165, Percent_Identity=31.5151515151515, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI195539395, Length=149, Percent_Identity=31.5436241610738, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI4506209, Length=212, Percent_Identity=26.4150943396226, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI31377644, Length=154, Percent_Identity=31.8181818181818, Blast_Score=67, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17563248, Length=218, Percent_Identity=27.0642201834862, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17508701, Length=213, Percent_Identity=26.7605633802817, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71987372, Length=151, Percent_Identity=31.1258278145695, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71987364, Length=151, Percent_Identity=31.1258278145695, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17554264, Length=194, Percent_Identity=30.4123711340206, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25146157, Length=170, Percent_Identity=31.1764705882353, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324833, Length=177, Percent_Identity=30.5084745762712, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324691, Length=320, Percent_Identity=25.9375, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6322704, Length=225, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320602, Length=310, Percent_Identity=25.4838709677419, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320077, Length=189, Percent_Identity=29.6296296296296, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325431, Length=149, Percent_Identity=31.5436241610738, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319554, Length=196, Percent_Identity=30.6122448979592, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321390, Length=228, Percent_Identity=25.4385964912281, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6324000, Length=189, Percent_Identity=26.4550264550265, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6325183, Length=218, Percent_Identity=27.0642201834862, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321465, Length=196, Percent_Identity=29.0816326530612, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24640100, Length=214, Percent_Identity=28.0373831775701, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI17738151, Length=213, Percent_Identity=28.169014084507, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19920408, Length=149, Percent_Identity=32.8859060402685, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI18860063, Length=293, Percent_Identity=25.2559726962457, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28573010, Length=245, Percent_Identity=27.7551020408163, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24651451, Length=146, Percent_Identity=31.5068493150685, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137738, Length=213, Percent_Identity=25.3521126760563, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281363136, Length=136, Percent_Identity=33.0882352941176, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI78707192, Length=136, Percent_Identity=33.0882352941176, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24663015, Length=146, Percent_Identity=31.5068493150685, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI17864540, Length=193, Percent_Identity=29.5336787564767, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24649446, Length=222, Percent_Identity=27.027027027027, Blast_Score=69, Evalue=8e-12,
Organism=Drosophila melanogaster, GI18859657, Length=264, Percent_Identity=28.4090909090909, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 67223; Mature: 67223

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHYLDFISCKDLTNNSLFGFLKCNENEGKILQFMAKALLEGEMDFVVHILLAKVFPHKD
CCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
TSSTQGEHSLAFLHHIRNLIELGWITQSSFLQPKLTDIALLELYNDNICLSHTFLKLLED
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEEEHHCCCEEHHHHHHHHHCC
GTLNVDLPEITPYEDHLEYLKDRFAHIDLLHKMSGLKFSQKIDSPSLLRANYRLKLLEDT
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEECCCHHHHHHHH
INSRLKLTTKDISIIKLIKEHHLEQKEQIIFFALLKEEYSSSDENLRDINALLELISLDE
HCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCH
YDKIKHRSLLDENSNLVQKGLVDYDELLNPFGGISRTFFIPEEILQDIIHPNKKKKRAKI
HHHHHHHHHHCCCHHHHHHHCCCHHHHHCCCCCCCEEEECHHHHHHHHHCCCCHHHHHHC
SLDSIIKEQEIFELVEPKQSINDVILHSHTREILDNLLAQVNSNVISLLRLWGIKDKKKG
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IDAKILLYGAAGTGKTITALALAKSLKKQVLSFDCSKILSMYVGESEKNVRKIFDSYQEI
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
CKKIKNEPILLLDEADQFLSTRATSGNSADKMHNQMQNIFLEQIERFEGILIATTNLLEN
HHHHCCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHH
FDSAFSRRFNYKIEFKRPNKEQRIALWLKYLPKNADFGEDCKENLANALAEYNLSGGQIH
HHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
LIVKNTAYKVATKKEPIFTQQDFITHIKAELSGNFDNAKSMGFRS
EEEECCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MKHYLDFISCKDLTNNSLFGFLKCNENEGKILQFMAKALLEGEMDFVVHILLAKVFPHKD
CCHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
TSSTQGEHSLAFLHHIRNLIELGWITQSSFLQPKLTDIALLELYNDNICLSHTFLKLLED
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHEEEHHCCCEEHHHHHHHHHCC
GTLNVDLPEITPYEDHLEYLKDRFAHIDLLHKMSGLKFSQKIDSPSLLRANYRLKLLEDT
CCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEECCCHHHHHHHH
INSRLKLTTKDISIIKLIKEHHLEQKEQIIFFALLKEEYSSSDENLRDINALLELISLDE
HCCCEEEEHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCH
YDKIKHRSLLDENSNLVQKGLVDYDELLNPFGGISRTFFIPEEILQDIIHPNKKKKRAKI
HHHHHHHHHHCCCHHHHHHHCCCHHHHHCCCCCCCEEEECHHHHHHHHHCCCCHHHHHHC
SLDSIIKEQEIFELVEPKQSINDVILHSHTREILDNLLAQVNSNVISLLRLWGIKDKKKG
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
IDAKILLYGAAGTGKTITALALAKSLKKQVLSFDCSKILSMYVGESEKNVRKIFDSYQEI
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
CKKIKNEPILLLDEADQFLSTRATSGNSADKMHNQMQNIFLEQIERFEGILIATTNLLEN
HHHHCCCCEEEECCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEHHHHHHH
FDSAFSRRFNYKIEFKRPNKEQRIALWLKYLPKNADFGEDCKENLANALAEYNLSGGQIH
HHHHHHHHCCEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
LIVKNTAYKVATKKEPIFTQQDFITHIKAELSGNFDNAKSMGFRS
EEEECCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]