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The map label for this gene is htrA

Identifier: 32265953

GI number: 32265953

Start: 435032

End: 436465

Strand: Direct

Name: htrA

Synonym: HH0454

Alternate gene names: 32265953

Gene position: 435032-436465 (Clockwise)

Preceding gene: 32265952

Following gene: 32265954

Centisome position: 24.18

GC content: 37.24

Gene sequence:

>1434_bases
ATGAAGTATGCAAGTATATATTTCTTTGTCAGTGCGCTATTTATGACTCATATTTTAGCTATTGAATTTCAAGAAGCGCC
CACAATCCAAAAACGTGTTAATCCTCAACAAAATAGCGATACACTCTACTCTTTTAACTCCTCCATAGAAAGTGCAAAAA
AAGCTGTTGTAAATATTTCTACACAAAAAAATGTCAGCAATCAACTTCCTAATCATCCGATGTTTAATGACCCATTTTTT
AAGCAATTTTTTGGTGATATTTATGGACAGATTCCCAAAGAACGTGTAGAACGTAGTTTGGGAAGTGGCGTAATTATTTC
AAATGATGGATACATTATTACAAACAATCACGTGATTGAAGACGCCGACAAAGTGCTTGTTTCACTTTCTGATAGCACAA
AAGAATACACTGCTAAAGTCATTGGCACAGATGCACGAAGCGATTTGGCGGTGATTAAAATTGAACAAAAAAACCTATCT
CCTATCACTTTTGCCCAAAGTAGCAATGTTCTTATAGGCGATGTAGTGTTTGCTATTGGGAATCCTTTTGGCGTAGGTGA
AACAATTACACAAGGTATTGTCTCTGCACTCAATAAAAGTGGTATTGGCATCAATGATTATGAAAATTTTATCCAAACTG
ATGCCTCAATCAATCCGGGTAATTCAGGTGGAGCATTAGTGGATTCTCGGGGAGCGCTCATTGGGATTAATACGGCTATC
CTCTCGCGCACAGGAGGTAATCACGGTGTAGGTTTTGCAATACCTTCAGATATGGCAAAAAAAATCGCCAAAGAACTCAT
TGAAAAAGGTGGGATTAAACGTGGATTCTTAGGCGTAGGAATCCAAGATATTAATAATGATATTAAAGAAAGTTATGGAG
ATAATAGTGGTGCAGTGGTTATTAGCCTAGAGCCTCAGTCTCCAGCAGCAAAAGCAGGGCTTATGGTGTGGGATTTAATC
ACTCACGTTAATGGCAAAAAAATCTCAAATGCAGCCGAACTTAAAAATCTTATCGGTATGCTCTCTCCTAATGAAAAAGT
TGTTGTGAAGTTTATCCGTGATAAACAAGAGCGTGTAACTCAAATCACACTTGGCGAATTACCCGATACAAAATCAAATC
AAACTTCTTCACAAAATACGCCAAATGGTTCTGCCTCAAGTGTAGAAGGCTTAAGTGTAGAATCGCTCAATCAAGCTCAA
CGCCAACGTTATAGAGTGCCAAATAATATTGATGGCGTAGTAGTTACTCGAGTCAATCCTAATTCAAAAGCTCAACAAGC
AGGATTTGAAGTGGGCGATATTATTGCACAAGTTGAAAATATAGCTATTAAAAAGCCAACAGACTTAACCAATGCATTTA
CACGCTTTAAGGGCAAAAACAAGAGAATCTTAGTTTATAGCGCTAATGGCACAAAAACAATTATGATGAAATAA

Upstream 100 bases:

>100_bases
GGTGTGCGAGAGATATTAGGAGATAGCTCTTTTAAAAATGATTAATAAGTGTTTAATTTAAACTTATAAAATCTCACAAT
TAAATGCTAAGGAGTATTTT

Downstream 100 bases:

>100_bases
CAAAAATGTAAGGAAATAAATTTGGTAATGCTTGATAATGCACAGATCTCCCTCATTAAGGAGCGTCTGTATAAATATAG
TGGTATTTTTTTAAATGATT

Product: serine protease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKYASIYFFVSALFMTHILAIEFQEAPTIQKRVNPQQNSDTLYSFNSSIESAKKAVVNISTQKNVSNQLPNHPMFNDPFF
KQFFGDIYGQIPKERVERSLGSGVIISNDGYIITNNHVIEDADKVLVSLSDSTKEYTAKVIGTDARSDLAVIKIEQKNLS
PITFAQSSNVLIGDVVFAIGNPFGVGETITQGIVSALNKSGIGINDYENFIQTDASINPGNSGGALVDSRGALIGINTAI
LSRTGGNHGVGFAIPSDMAKKIAKELIEKGGIKRGFLGVGIQDINNDIKESYGDNSGAVVISLEPQSPAAKAGLMVWDLI
THVNGKKISNAAELKNLIGMLSPNEKVVVKFIRDKQERVTQITLGELPDTKSNQTSSQNTPNGSASSVEGLSVESLNQAQ
RQRYRVPNNIDGVVVTRVNPNSKAQQAGFEVGDIIAQVENIAIKKPTDLTNAFTRFKGKNKRILVYSANGTKTIMMK

Sequences:

>Translated_477_residues
MKYASIYFFVSALFMTHILAIEFQEAPTIQKRVNPQQNSDTLYSFNSSIESAKKAVVNISTQKNVSNQLPNHPMFNDPFF
KQFFGDIYGQIPKERVERSLGSGVIISNDGYIITNNHVIEDADKVLVSLSDSTKEYTAKVIGTDARSDLAVIKIEQKNLS
PITFAQSSNVLIGDVVFAIGNPFGVGETITQGIVSALNKSGIGINDYENFIQTDASINPGNSGGALVDSRGALIGINTAI
LSRTGGNHGVGFAIPSDMAKKIAKELIEKGGIKRGFLGVGIQDINNDIKESYGDNSGAVVISLEPQSPAAKAGLMVWDLI
THVNGKKISNAAELKNLIGMLSPNEKVVVKFIRDKQERVTQITLGELPDTKSNQTSSQNTPNGSASSVEGLSVESLNQAQ
RQRYRVPNNIDGVVVTRVNPNSKAQQAGFEVGDIIAQVENIAIKKPTDLTNAFTRFKGKNKRILVYSANGTKTIMMK
>Mature_477_residues
MKYASIYFFVSALFMTHILAIEFQEAPTIQKRVNPQQNSDTLYSFNSSIESAKKAVVNISTQKNVSNQLPNHPMFNDPFF
KQFFGDIYGQIPKERVERSLGSGVIISNDGYIITNNHVIEDADKVLVSLSDSTKEYTAKVIGTDARSDLAVIKIEQKNLS
PITFAQSSNVLIGDVVFAIGNPFGVGETITQGIVSALNKSGIGINDYENFIQTDASINPGNSGGALVDSRGALIGINTAI
LSRTGGNHGVGFAIPSDMAKKIAKELIEKGGIKRGFLGVGIQDINNDIKESYGDNSGAVVISLEPQSPAAKAGLMVWDLI
THVNGKKISNAAELKNLIGMLSPNEKVVVKFIRDKQERVTQITLGELPDTKSNQTSSQNTPNGSASSVEGLSVESLNQAQ
RQRYRVPNNIDGVVVTRVNPNSKAQQAGFEVGDIIAQVENIAIKKPTDLTNAFTRFKGKNKRILVYSANGTKTIMMK

Specific function: Serine Protease That Is Required At High Temperature. Involved In The Degradation Of Damaged Proteins. It Can Degrade Icia, Ada, Casein And Globin. Shared Specificity With Degq. [C]

COG id: COG0265

COG function: function code O; Trypsin-like serine proteases, typically periplasmic, contain C-terminal PDZ domain

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PDZ (DHR) domains [H]

Homologues:

Organism=Homo sapiens, GI4506141, Length=272, Percent_Identity=34.5588235294118, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI22129776, Length=367, Percent_Identity=32.6975476839237, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI24308541, Length=251, Percent_Identity=35.0597609561753, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI7019477, Length=257, Percent_Identity=33.4630350194552, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1786356, Length=355, Percent_Identity=44.5070422535211, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1789629, Length=370, Percent_Identity=41.6216216216216, Blast_Score=252, Evalue=5e-68,
Organism=Escherichia coli, GI1789630, Length=370, Percent_Identity=37.5675675675676, Blast_Score=206, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24646839, Length=263, Percent_Identity=32.6996197718631, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR009003
- InterPro:   IPR011782
- InterPro:   IPR001254
- InterPro:   IPR001940 [H]

Pfam domain/function: PF00595 PDZ; PF00089 Trypsin [H]

EC number: 3.4.21.-

Molecular weight: Translated: 51571; Mature: 51571

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS50106 PDZ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYASIYFFVSALFMTHILAIEFQEAPTIQKRVNPQQNSDTLYSFNSSIESAKKAVVNIS
CCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHCCCCCCCCCEEEEHHHHHHHHHHEEEEC
TQKNVSNQLPNHPMFNDPFFKQFFGDIYGQIPKERVERSLGSGVIISNDGYIITNNHVIE
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCEEEECCCEEC
DADKVLVSLSDSTKEYTAKVIGTDARSDLAVIKIEQKNLSPITFAQSSNVLIGDVVFAIG
CCCEEEEEECCCCHHHHHHEECCCCCCCEEEEEEECCCCCEEEEECCCCEEEEEEHHECC
NPFGVGETITQGIVSALNKSGIGINDYENFIQTDASINPGNSGGALVDSRGALIGINTAI
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEHHE
LSRTGGNHGVGFAIPSDMAKKIAKELIEKGGIKRGFLGVGIQDINNDIKESYGDNSGAVV
EECCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHCCCCCCEEE
ISLEPQSPAAKAGLMVWDLITHVNGKKISNAAELKNLIGMLSPNEKVVVKFIRDKQERVT
EEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QITLGELPDTKSNQTSSQNTPNGSASSVEGLSVESLNQAQRQRYRVPNNIDGVVVTRVNP
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECC
NSKAQQAGFEVGDIIAQVENIAIKKPTDLTNAFTRFKGKNKRILVYSANGTKTIMMK
CCHHHHCCCHHHHHHHHHHHHEECCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEC
>Mature Secondary Structure
MKYASIYFFVSALFMTHILAIEFQEAPTIQKRVNPQQNSDTLYSFNSSIESAKKAVVNIS
CCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHCCCCCCCCCEEEEHHHHHHHHHHEEEEC
TQKNVSNQLPNHPMFNDPFFKQFFGDIYGQIPKERVERSLGSGVIISNDGYIITNNHVIE
HHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCEEEECCCEEEECCCEEC
DADKVLVSLSDSTKEYTAKVIGTDARSDLAVIKIEQKNLSPITFAQSSNVLIGDVVFAIG
CCCEEEEEECCCCHHHHHHEECCCCCCCEEEEEEECCCCCEEEEECCCCEEEEEEHHECC
NPFGVGETITQGIVSALNKSGIGINDYENFIQTDASINPGNSGGALVDSRGALIGINTAI
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCEEECCCCEEEEEHHE
LSRTGGNHGVGFAIPSDMAKKIAKELIEKGGIKRGFLGVGIQDINNDIKESYGDNSGAVV
EECCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHCCCCCCEEE
ISLEPQSPAAKAGLMVWDLITHVNGKKISNAAELKNLIGMLSPNEKVVVKFIRDKQERVT
EEECCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QITLGELPDTKSNQTSSQNTPNGSASSVEGLSVESLNQAQRQRYRVPNNIDGVVVTRVNP
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECC
NSKAQQAGFEVGDIIAQVENIAIKKPTDLTNAFTRFKGKNKRILVYSANGTKTIMMK
CCHHHHCCCHHHHHHHHHHHHEECCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases) [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7861951 [H]