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The map label for this gene is 32265837

Identifier: 32265837

GI number: 32265837

Start: 332213

End: 333103

Strand: Direct

Name: 32265837

Synonym: HH0338

Alternate gene names: NA

Gene position: 332213-333103 (Clockwise)

Preceding gene: 32265836

Following gene: 32265838

Centisome position: 18.47

GC content: 38.83

Gene sequence:

>891_bases
ATGAAAATGCCCTCTATGATCCGTGTAGCACTTGTGCAGCAAGCCTACAAAGGAAATAAAGAGGCAATGATAGAATCAAG
TGCTGCAATGATTGCTAAAGCGGCACATTCTAACGCACAACTTGTAGCATTACAAGAGCTTCATACACACGAATATTTTT
GTCAAAGTGAGAATCCACAATTTTTTGATTATGCAATGGATTTTGATAAAGATGTGGCATATTTTAGCGCATTAGCTAAA
AAGCATAAAATCGTGCTTATTACTTCACTTTTTGAAAAACGTGCTGCAGGGCTATATCACAATACCGCTGTTGTTTTTGA
TATTGATGGCTCTATAGCTGGAAAATACCGCAAAATGCACATTCCTGATGACCCACAATTTTATGAGAAATTCTACTTCA
CACCCGGTGATTTAGGATTTGAGCCCATATCTACAAGTTTAGGGAAACTTGGTGTGCTTATTTGCTGGGACCAATGGTAT
CCCGAAGCAGCACGTATTATGGCACTAAAGGGAGCGCAAATGCTTATTTATCCTACTGCTATTGGGTGGTTTGATGAAGA
TACTTTAGAGGAGAAAACACGCCAAAAAGAAGCGTGGATAGCTGTGCAAAGAGGACATAGTGTTGCGAATGGATTACCTA
CAATGGCAATTAACCGCGTGGGTTTTGAATCTGATAGCAGTAAGGTAGGGAATGGCATACGATTTTGGGGTTCAAGCTTT
GTATTTGGTGCACAAGGTGAGCTTTTGGCACAAGGAAGTGAGAATAAAGAAGAAATTATTTTAGTAGAAATTGATTTACA
AAGAAGTGAAGAAGTGCGCCGTATGTGGCCATTTTTACGCGATAGACGCATAGAATCTTATAGTGAAATTCTTAAACGAT
TTTGTGATTAA

Upstream 100 bases:

>100_bases
TGAGGATTTCGAAGATTATGATGATGATTATTCATACAATGCGTATGATGATGACGATTATCAAAGCCCCGATAGCGAAT
ATGATGAATAAGGATTTTTA

Downstream 100 bases:

>100_bases
AACTATTTTAATACCCTATTAAATATGCTTATTATTCAACATAATTTAATGTTTATAGGTATAGAATAACCCGTTTCAAT
GAAACAATCTTTACAAAAGG

Product: hypothetical protein

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MKMPSMIRVALVQQAYKGNKEAMIESSAAMIAKAAHSNAQLVALQELHTHEYFCQSENPQFFDYAMDFDKDVAYFSALAK
KHKIVLITSLFEKRAAGLYHNTAVVFDIDGSIAGKYRKMHIPDDPQFYEKFYFTPGDLGFEPISTSLGKLGVLICWDQWY
PEAARIMALKGAQMLIYPTAIGWFDEDTLEEKTRQKEAWIAVQRGHSVANGLPTMAINRVGFESDSSKVGNGIRFWGSSF
VFGAQGELLAQGSENKEEIILVEIDLQRSEEVRRMWPFLRDRRIESYSEILKRFCD

Sequences:

>Translated_296_residues
MKMPSMIRVALVQQAYKGNKEAMIESSAAMIAKAAHSNAQLVALQELHTHEYFCQSENPQFFDYAMDFDKDVAYFSALAK
KHKIVLITSLFEKRAAGLYHNTAVVFDIDGSIAGKYRKMHIPDDPQFYEKFYFTPGDLGFEPISTSLGKLGVLICWDQWY
PEAARIMALKGAQMLIYPTAIGWFDEDTLEEKTRQKEAWIAVQRGHSVANGLPTMAINRVGFESDSSKVGNGIRFWGSSF
VFGAQGELLAQGSENKEEIILVEIDLQRSEEVRRMWPFLRDRRIESYSEILKRFCD
>Mature_296_residues
MKMPSMIRVALVQQAYKGNKEAMIESSAAMIAKAAHSNAQLVALQELHTHEYFCQSENPQFFDYAMDFDKDVAYFSALAK
KHKIVLITSLFEKRAAGLYHNTAVVFDIDGSIAGKYRKMHIPDDPQFYEKFYFTPGDLGFEPISTSLGKLGVLICWDQWY
PEAARIMALKGAQMLIYPTAIGWFDEDTLEEKTRQKEAWIAVQRGHSVANGLPTMAINRVGFESDSSKVGNGIRFWGSSF
VFGAQGELLAQGSENKEEIILVEIDLQRSEEVRRMWPFLRDRRIESYSEILKRFCD

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=292, Percent_Identity=29.4520547945205, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI7706509, Length=314, Percent_Identity=27.0700636942675, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI297632350, Length=292, Percent_Identity=23.2876712328767, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI297632348, Length=292, Percent_Identity=23.2876712328767, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI5031947, Length=292, Percent_Identity=23.2876712328767, Blast_Score=76, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17533173, Length=311, Percent_Identity=28.2958199356913, Blast_Score=124, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI17556280, Length=289, Percent_Identity=26.643598615917, Blast_Score=91, Evalue=7e-19,
Organism=Saccharomyces cerevisiae, GI6323383, Length=300, Percent_Identity=29.6666666666667, Blast_Score=107, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21358471, Length=311, Percent_Identity=28.9389067524116, Blast_Score=121, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17933642, Length=285, Percent_Identity=24.2105263157895, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21355835, Length=296, Percent_Identity=25, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33709; Mature: 33709

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMPSMIRVALVQQAYKGNKEAMIESSAAMIAKAAHSNAQLVALQELHTHEYFCQSENPQ
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCC
FFDYAMDFDKDVAYFSALAKKHKIVLITSLFEKRAAGLYHNTAVVFDIDGSIAGKYRKMH
EEEHECCCCHHHHHHHHHHHCCEEEEEEHHHHHHHCCCEECEEEEEEECCCCCCCEEEEC
IPDDPQFYEKFYFTPGDLGFEPISTSLGKLGVLICWDQWYPEAARIMALKGAQMLIYPTA
CCCCHHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHEEEECCCEEEEEEEE
IGWFDEDTLEEKTRQKEAWIAVQRGHSVANGLPTMAINRVGFESDSSKVGNGIRFWGSSF
CCCCCCHHHHHHHHHHHHEEEEECCCHHHCCCCHHEEHHCCCCCCCHHHCCCEEECCCCE
VFGAQGELLAQGSENKEEIILVEIDLQRSEEVRRMWPFLRDRRIESYSEILKRFCD
EECCCCCEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKMPSMIRVALVQQAYKGNKEAMIESSAAMIAKAAHSNAQLVALQELHTHEYFCQSENPQ
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCC
FFDYAMDFDKDVAYFSALAKKHKIVLITSLFEKRAAGLYHNTAVVFDIDGSIAGKYRKMH
EEEHECCCCHHHHHHHHHHHCCEEEEEEHHHHHHHCCCEECEEEEEEECCCCCCCEEEEC
IPDDPQFYEKFYFTPGDLGFEPISTSLGKLGVLICWDQWYPEAARIMALKGAQMLIYPTA
CCCCHHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEECCCCCCHHHEEEECCCEEEEEEEE
IGWFDEDTLEEKTRQKEAWIAVQRGHSVANGLPTMAINRVGFESDSSKVGNGIRFWGSSF
CCCCCCHHHHHHHHHHHHEEEEECCCHHHCCCCHHEEHHCCCCCCCHHHCCCEEECCCCE
VFGAQGELLAQGSENKEEIILVEIDLQRSEEVRRMWPFLRDRRIESYSEILKRFCD
EECCCCCEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]