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The map label for this gene is nrfA [H]

Identifier: 32265716

GI number: 32265716

Start: 206284

End: 207768

Strand: Direct

Name: nrfA [H]

Synonym: HH0217

Alternate gene names: 32265716

Gene position: 206284-207768 (Clockwise)

Preceding gene: 32265715

Following gene: 32265717

Centisome position: 11.47

GC content: 39.87

Gene sequence:

>1485_bases
ATGGACACTGCGCGTAATAATAAAGACTTTCTTAATGCACACGGCTTTGCTAAATTTGGTGGGCAACCTGCAGCTTGTAT
GAATTGCCATAGTGGCTGGACTCCGTGGCTTATCAAAAATGCAGCAAATGGCGATTTTACTGCGTTTAATAGCACAAAAT
ATTGGACAATGATTAAAAATGTCCCCACTCGTAATGGGATTGAAGAAGGTTCTGAAGAGCACGGAGGACCACACGGCGGT
AAAAGAATGGGTGTAACTTGTGCAGATTGCCATAACCCTGATGATATGAGTCTAAGAGTAGTGCGCCCTGCTCTTATTAA
TGCACTCGTTGATAGAGGTTACCAAAAAGATGCAGCACAAGGTATTAAAGCAGATAGAAAAGAGATGAGGACACTTGTTT
GCGCTCAATGCCACGTAGAATATTATTTCAAACCTACTGGTGAAAAAGTTAAGGTAATGGGAGAATCTATTGCATCTGAT
TCGAGTAAAAAATGGTGGAATGGCACACAAAAAACTTATGATGAGTTTGAACATTGGAGAGATGGCAATAAAGCAACTGA
AATTGAAGTTGATGGTATCAATCTTACATTCCCTTGGAGTGCGTGGAAAAAAGGTGAGGTGTTTAGAATTGAAATGTTTG
ACGAACATTATGATGCAGTGCGCCCCATTTTCAAAGAAGATTGGACACACAAGGAAACTAAAGCACCTATGCTCAAGATT
CAACACCCAGAATATGAAATGTTTAGCGGTGGTGTGCACGCAGCAAATGGTGTAAGCTGCGCGGATTGCCATATGCCTTA
CATTAAAGGCGCGGGAGGCAAAAAAGTAACGCAACACAATGTTACCTCTCCACTCTTTGATATTAATTCTGCTTGCAAAA
CTTGCCACACACAATCCGAAGAGTATCTTAAAAAACAAATTGCCGATATTCAAAAATCAGTAGCATTTGATTTGAGAAGT
GCAGAATATGCAACCGTGAGCCTTATTTTTGATATTAAAAAATTACGAGAAGAACTTGGCAAACTTCCAGCGTATCAGTC
TGAAGGCAAACCTGATGATACTAAAATATCAAAAGCTCTCGCAGAGCCACTTGAGCTACACCGCCGCGGACAAATGAGAG
GAGATTTTGTAGGAGCGGAAAACTCTACAGGATTCCATAATCCACGCGAAGCAAGCCGTATGCTCCTTCAAGCAGTAGAA
ATGGCGAGAATGGGGCAAACAAAATGTGTTGAAATTGCAAATGCAAATGGCATTAAAGGTTTTAAAACTTCTAATCTTGG
ATTTGATGAGATTCAAAAATTTAATCCTGGAGAGATTGTATATAAAATTGACCTTAATGGACACACTGCCGGAGAGCGCT
ATTACAAACATCACGAAATCAATGGTGAAGCACCTCAAGAACTTTTAGAGCTTGATAAAAATACAAAGCCTTATAACTTT
AGTGAAGTTGATAAAATCTCTGCAAGTGCCAAAAGCGCAAAATAG

Upstream 100 bases:

>100_bases
GTTGATTCGTTACCCACAATTAACAATATTATGGGCAGGGTATCCATTCAGTCTTGATGCTAATGAAGAAAGAGGGCATT
TTTGGATACAAGTAGATCAA

Downstream 100 bases:

>100_bases
TTTTCCTCTCTAGCCTCCATTTTAAAAGCGGAGGCTAGGATTCTTCCTCTTATATTGAAACATTTATGTTATAATCATTT
CATCTTGCCTCAAAGGAATT

Product: hypothetical protein

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MDTARNNKDFLNAHGFAKFGGQPAACMNCHSGWTPWLIKNAANGDFTAFNSTKYWTMIKNVPTRNGIEEGSEEHGGPHGG
KRMGVTCADCHNPDDMSLRVVRPALINALVDRGYQKDAAQGIKADRKEMRTLVCAQCHVEYYFKPTGEKVKVMGESIASD
SSKKWWNGTQKTYDEFEHWRDGNKATEIEVDGINLTFPWSAWKKGEVFRIEMFDEHYDAVRPIFKEDWTHKETKAPMLKI
QHPEYEMFSGGVHAANGVSCADCHMPYIKGAGGKKVTQHNVTSPLFDINSACKTCHTQSEEYLKKQIADIQKSVAFDLRS
AEYATVSLIFDIKKLREELGKLPAYQSEGKPDDTKISKALAEPLELHRRGQMRGDFVGAENSTGFHNPREASRMLLQAVE
MARMGQTKCVEIANANGIKGFKTSNLGFDEIQKFNPGEIVYKIDLNGHTAGERYYKHHEINGEAPQELLELDKNTKPYNF
SEVDKISASAKSAK

Sequences:

>Translated_494_residues
MDTARNNKDFLNAHGFAKFGGQPAACMNCHSGWTPWLIKNAANGDFTAFNSTKYWTMIKNVPTRNGIEEGSEEHGGPHGG
KRMGVTCADCHNPDDMSLRVVRPALINALVDRGYQKDAAQGIKADRKEMRTLVCAQCHVEYYFKPTGEKVKVMGESIASD
SSKKWWNGTQKTYDEFEHWRDGNKATEIEVDGINLTFPWSAWKKGEVFRIEMFDEHYDAVRPIFKEDWTHKETKAPMLKI
QHPEYEMFSGGVHAANGVSCADCHMPYIKGAGGKKVTQHNVTSPLFDINSACKTCHTQSEEYLKKQIADIQKSVAFDLRS
AEYATVSLIFDIKKLREELGKLPAYQSEGKPDDTKISKALAEPLELHRRGQMRGDFVGAENSTGFHNPREASRMLLQAVE
MARMGQTKCVEIANANGIKGFKTSNLGFDEIQKFNPGEIVYKIDLNGHTAGERYYKHHEINGEAPQELLELDKNTKPYNF
SEVDKISASAKSAK
>Mature_494_residues
MDTARNNKDFLNAHGFAKFGGQPAACMNCHSGWTPWLIKNAANGDFTAFNSTKYWTMIKNVPTRNGIEEGSEEHGGPHGG
KRMGVTCADCHNPDDMSLRVVRPALINALVDRGYQKDAAQGIKADRKEMRTLVCAQCHVEYYFKPTGEKVKVMGESIASD
SSKKWWNGTQKTYDEFEHWRDGNKATEIEVDGINLTFPWSAWKKGEVFRIEMFDEHYDAVRPIFKEDWTHKETKAPMLKI
QHPEYEMFSGGVHAANGVSCADCHMPYIKGAGGKKVTQHNVTSPLFDINSACKTCHTQSEEYLKKQIADIQKSVAFDLRS
AEYATVSLIFDIKKLREELGKLPAYQSEGKPDDTKISKALAEPLELHRRGQMRGDFVGAENSTGFHNPREASRMLLQAVE
MARMGQTKCVEIANANGIKGFKTSNLGFDEIQKFNPGEIVYKIDLNGHTAGERYYKHHEINGEAPQELLELDKNTKPYNF
SEVDKISASAKSAK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=360, Percent_Identity=29.7222222222222, Blast_Score=142, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 55461; Mature: 55461

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTARNNKDFLNAHGFAKFGGQPAACMNCHSGWTPWLIKNAANGDFTAFNSTKYWTMIKN
CCCCCCCCCHHCCCCHHHHCCCCCHHHCCCCCCCCCEEECCCCCCEEEECCCEEEEEECC
VPTRNGIEEGSEEHGGPHGGKRMGVTCADCHNPDDMSLRVVRPALINALVDRGYQKDAAQ
CCCCCCCCCCHHHCCCCCCCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHCCCHHHHHH
GIKADRKEMRTLVCAQCHVEYYFKPTGEKVKVMGESIASDSSKKWWNGTQKTYDEFEHWR
HHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEHHHHHHCCCCCHHCCCCHHHHHHHHHHC
DGNKATEIEVDGINLTFPWSAWKKGEVFRIEMFDEHYDAVRPIFKEDWTHKETKAPMLKI
CCCCEEEEEECCEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCHHCCCEEEE
QHPEYEMFSGGVHAANGVSCADCHMPYIKGAGGKKVTQHNVTSPLFDINSACKTCHTQSE
ECCCHHHHCCCCCCCCCCCHHHCCCCCEECCCCCCEECCCCCCCHHHHHHHHHHHHCCHH
EYLKKQIADIQKSVAFDLRSAEYATVSLIFDIKKLREELGKLPAYQSEGKPDDTKISKAL
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AEPLELHRRGQMRGDFVGAENSTGFHNPREASRMLLQAVEMARMGQTKCVEIANANGIKG
HHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
FKTSNLGFDEIQKFNPGEIVYKIDLNGHTAGERYYKHHEINGEAPQELLELDKNTKPYNF
CCCCCCCHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCH
SEVDKISASAKSAK
HHHHHHHCCCCCCC
>Mature Secondary Structure
MDTARNNKDFLNAHGFAKFGGQPAACMNCHSGWTPWLIKNAANGDFTAFNSTKYWTMIKN
CCCCCCCCCHHCCCCHHHHCCCCCHHHCCCCCCCCCEEECCCCCCEEEECCCEEEEEECC
VPTRNGIEEGSEEHGGPHGGKRMGVTCADCHNPDDMSLRVVRPALINALVDRGYQKDAAQ
CCCCCCCCCCHHHCCCCCCCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHCCCHHHHHH
GIKADRKEMRTLVCAQCHVEYYFKPTGEKVKVMGESIASDSSKKWWNGTQKTYDEFEHWR
HHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEHHHHHHCCCCCHHCCCCHHHHHHHHHHC
DGNKATEIEVDGINLTFPWSAWKKGEVFRIEMFDEHYDAVRPIFKEDWTHKETKAPMLKI
CCCCEEEEEECCEEEEECCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCHHCCCEEEE
QHPEYEMFSGGVHAANGVSCADCHMPYIKGAGGKKVTQHNVTSPLFDINSACKTCHTQSE
ECCCHHHHCCCCCCCCCCCHHHCCCCCEECCCCCCEECCCCCCCHHHHHHHHHHHHCCHH
EYLKKQIADIQKSVAFDLRSAEYATVSLIFDIKKLREELGKLPAYQSEGKPDDTKISKAL
HHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
AEPLELHRRGQMRGDFVGAENSTGFHNPREASRMLLQAVEMARMGQTKCVEIANANGIKG
HHHHHHHHCCCCCCCEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
FKTSNLGFDEIQKFNPGEIVYKIDLNGHTAGERYYKHHEINGEAPQELLELDKNTKPYNF
CCCCCCCHHHHHCCCCCCEEEEEECCCCCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCH
SEVDKISASAKSAK
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA