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The map label for this gene is napB

Identifier: 32265660

GI number: 32265660

Start: 156013

End: 156576

Strand: Direct

Name: napB

Synonym: HH0161

Alternate gene names: 32265660

Gene position: 156013-156576 (Clockwise)

Preceding gene: 32265659

Following gene: 32265661

Centisome position: 8.67

GC content: 38.12

Gene sequence:

>564_bases
ATGAAAAAATACGCTCAAAGTTTAATAATTGGGTTACTTGGATTGGCATTTTTCGCGTGTTCAGATTCTACGGAATCTAA
AAGTGATAATGTGTCTAAAGAGATACAAGGTGAAATGTTTAAAGATAGTGAAATTGGCTTAAGAAAAGTGGATTTGCAAG
ATGAAAAAGATGTCAAATTGCTTCAATATGCATATCCTAATGCTTCTGCAGGCGAGAGCACACTTATTGAACGCTCATTT
GAAAATGCTCCACCTATGATTTCTCACAGCATAGAAGGTATGCTACCTATCACAAAAGATGATAATCAATGTCTTACTTG
CCACGACCCTGCAGTTGCAGCAGATGTAGGTGCTATAGCCGTTCCTGCTTCGCATACTTATGATTTGCGCACAAATAAAA
ATTTAGGCGAAGTCTCTCACGCACGATTTAATTGTGTGTTATGTCATACTCCACAGGCAGATGTAGCACCAGCTATTGCC
AATACTTTTTCACCTGCTTTCCGCACTCAAGAGGGCAAAAAATCATCAAATCTTCTTGATGTTCTTAATGAGGGCGTAAA
TTAA

Upstream 100 bases:

>100_bases
TAAAAACAATGACTTGTTTGCGCTGTGGGAGATGTATTGAGGTGTGCGATGATAACGCACTGAATTTTAGTATTTTAAAT
TTTACACACAAGGAGAAAAG

Downstream 100 bases:

>100_bases
GGTTAAATAAAGTGGATTCTCTACAAAGATGCACACGAAAGTTTAGCACACAAAGACTATTGTGCTGGCTTTTGTGTTTT
TTTGCTTTATGTTTGCCCCT

Product: periplasmic nitrate reductase

Products: nitrate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MKKYAQSLIIGLLGLAFFACSDSTESKSDNVSKEIQGEMFKDSEIGLRKVDLQDEKDVKLLQYAYPNASAGESTLIERSF
ENAPPMISHSIEGMLPITKDDNQCLTCHDPAVAADVGAIAVPASHTYDLRTNKNLGEVSHARFNCVLCHTPQADVAPAIA
NTFSPAFRTQEGKKSSNLLDVLNEGVN

Sequences:

>Translated_187_residues
MKKYAQSLIIGLLGLAFFACSDSTESKSDNVSKEIQGEMFKDSEIGLRKVDLQDEKDVKLLQYAYPNASAGESTLIERSF
ENAPPMISHSIEGMLPITKDDNQCLTCHDPAVAADVGAIAVPASHTYDLRTNKNLGEVSHARFNCVLCHTPQADVAPAIA
NTFSPAFRTQEGKKSSNLLDVLNEGVN
>Mature_187_residues
MKKYAQSLIIGLLGLAFFACSDSTESKSDNVSKEIQGEMFKDSEIGLRKVDLQDEKDVKLLQYAYPNASAGESTLIERSF
ENAPPMISHSIEGMLPITKDDNQCLTCHDPAVAADVGAIAVPASHTYDLRTNKNLGEVSHARFNCVLCHTPQADVAPAIA
NTFSPAFRTQEGKKSSNLLDVLNEGVN

Specific function: Small subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme NapC protein, thus allowing electron flow between membrane and periplasm.

COG id: COG3043

COG function: function code C; Nitrate reductase cytochrome c-type subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082065, Length=88, Percent_Identity=40.9090909090909, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011031
- InterPro:   IPR005591 [H]

Pfam domain/function: PF03892 NapB [H]

EC number: 1.7.99.4

Molecular weight: Translated: 20275; Mature: 20275

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYAQSLIIGLLGLAFFACSDSTESKSDNVSKEIQGEMFKDSEIGLRKVDLQDEKDVKL
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCCCCCEE
LQYAYPNASAGESTLIERSFENAPPMISHSIEGMLPITKDDNQCLTCHDPAVAADVGAIA
EEEECCCCCCCCHHHHHHHHCCCCCHHHHCCCCEECCCCCCCCEEEECCCCHHHCCCEEE
VPASHTYDLRTNKNLGEVSHARFNCVLCHTPQADVAPAIANTFSPAFRTQEGKKSSNLLD
ECCCCEEECCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCHHHHCCCCCCHHHHHH
VLNEGVN
HHHCCCC
>Mature Secondary Structure
MKKYAQSLIIGLLGLAFFACSDSTESKSDNVSKEIQGEMFKDSEIGLRKVDLQDEKDVKL
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEECCCCCCCCEE
LQYAYPNASAGESTLIERSFENAPPMISHSIEGMLPITKDDNQCLTCHDPAVAADVGAIA
EEEECCCCCCCCHHHHHHHHCCCCCHHHHCCCCEECCCCCCCCEEEECCCCHHHCCCEEE
VPASHTYDLRTNKNLGEVSHARFNCVLCHTPQADVAPAIANTFSPAFRTQEGKKSSNLLD
ECCCCEEECCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHCCHHHHCCCCCCHHHHHH
VLNEGVN
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite; acceptor

Specific reaction: nitrite + acceptor = nitrate + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 8039676 [H]