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The map label for this gene is rpoE [C]

Identifier: 30995451

GI number: 30995451

Start: 1542231

End: 1542776

Strand: Reverse

Name: rpoE [C]

Synonym: HI1459

Alternate gene names: 30995451

Gene position: 1542776-1542231 (Counterclockwise)

Preceding gene: 30995452

Following gene: 16273363

Centisome position: 84.3

GC content: 33.15

Gene sequence:

>546_bases
ATGAATGTTATTTCTGATCTTGAGCTTCAGCAAATTCGCACTCAAATGCTGACATTTGCACAATTGCAAGTAAATCAAGC
CGATCTTGCTGAAGATTTAGTACAAGAAGCTTTTTTAAGTGCATTCAAAAATCTCGCTAATTTTAAGCGTCAATCTGCAT
TTAAGACTTGGATTTTTGCGATTCTTAAAAATAAGATTATTGATTATTTACGTCAGAAAGGGCGGTTTGTTTTAGAAAGT
GAACTTGAAGATGAAAATACTAATAATTCATTTTTTGATGAAAAGGGGCATTGGAAACCAGAATATCACCCGAGTGAATT
ACAAGGCGAAGAAGAAACGGTTTATTCCGATGAATTTTGGCTGATTTTTGAAACTTGTTTGAATTGTTTACCTGCTAAAC
AGGCAAAAATTTTTATGATGCGAGAGTTTTTAGAGCTTTCATCTGAAGAAATTTGTCAAGAAACACATTTAACTTCGTCT
AATTTACATACCACCCTTTATCGGGCTCGTTTACAGTTGCAAAACTGTCTTTCAAAAAAACTTTAG

Upstream 100 bases:

>100_bases
TTTTTCATTTTAAATTTCCTTTTTTGAGTGTAAAAATAGCTACCTTAGTTTGTTAAAAGAAGTGAGTTTCATTTCTTACA
TAAGTTTTAAAGGAATTAAA

Downstream 100 bases:

>100_bases
GAGAAAATTATGCGTTGTCACCAAGCCACTCGAATGATTTCCGAATCGCACGAACGTTCATTAGATATACAAGAAAAAGT
TGGGTTAAGAGTTCATTTAG

Product: RNA polymerase sigma factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MNVISDLELQQIRTQMLTFAQLQVNQADLAEDLVQEAFLSAFKNLANFKRQSAFKTWIFAILKNKIIDYLRQKGRFVLES
ELEDENTNNSFFDEKGHWKPEYHPSELQGEEETVYSDEFWLIFETCLNCLPAKQAKIFMMREFLELSSEEICQETHLTSS
NLHTTLYRARLQLQNCLSKKL

Sequences:

>Translated_181_residues
MNVISDLELQQIRTQMLTFAQLQVNQADLAEDLVQEAFLSAFKNLANFKRQSAFKTWIFAILKNKIIDYLRQKGRFVLES
ELEDENTNNSFFDEKGHWKPEYHPSELQGEEETVYSDEFWLIFETCLNCLPAKQAKIFMMREFLELSSEEICQETHLTSS
NLHTTLYRARLQLQNCLSKKL
>Mature_181_residues
MNVISDLELQQIRTQMLTFAQLQVNQADLAEDLVQEAFLSAFKNLANFKRQSAFKTWIFAILKNKIIDYLRQKGRFVLES
ELEDENTNNSFFDEKGHWKPEYHPSELQGEEETVYSDEFWLIFETCLNCLPAKQAKIFMMREFLELSSEEICQETHLTSS
NLHTTLYRARLQLQNCLSKKL

Specific function: Sigma Factors Are Initiation Factors That Promote The Attachment Of RNA Polymerase To Specific Initiation Sites And Are Then Released. This Sigma Factor Is Involved In Heat Shock And Oxidative Stress Response; It Is Believed To Control Protein Processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily

Homologues:

Organism=Escherichia coli, GI1788926, Length=144, Percent_Identity=29.8611111111111, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): Y1459_HAEIN (P45215)

Other databases:

- EMBL:   L42023
- PIR:   G64124
- RefSeq:   NP_439610.2
- ProteinModelPortal:   P45215
- SMR:   P45215
- GeneID:   950350
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI1459
- NMPDR:   fig|71421.1.peg.1391
- TIGR:   HI_1459
- HOGENOM:   HBG296729
- OMA:   WIFAILK
- ProtClustDB:   PRK12530
- BioCyc:   HINF71421:HI_1459-MONOMER
- InterPro:   IPR014289
- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- TIGRFAMs:   TIGR02943
- TIGRFAMs:   TIGR02937

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2; SSF88946 Sigma_r2; SSF88659 Sigma_r3_r4

EC number: NA

Molecular weight: Translated: 21341; Mature: 21341

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVISDLELQQIRTQMLTFAQLQVNQADLAEDLVQEAFLSAFKNLANFKRQSAFKTWIFA
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKNKIIDYLRQKGRFVLESELEDENTNNSFFDEKGHWKPEYHPSELQGEEETVYSDEFW
HHHHHHHHHHHHCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHEECCHHH
LIFETCLNCLPAKQAKIFMMREFLELSSEEICQETHLTSSNLHTTLYRARLQLQNCLSKK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
L
C
>Mature Secondary Structure
MNVISDLELQQIRTQMLTFAQLQVNQADLAEDLVQEAFLSAFKNLANFKRQSAFKTWIFA
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ILKNKIIDYLRQKGRFVLESELEDENTNNSFFDEKGHWKPEYHPSELQGEEETVYSDEFW
HHHHHHHHHHHHCCCEEEHHHCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCHHEECCHHH
LIFETCLNCLPAKQAKIFMMREFLELSSEEICQETHLTSSNLHTTLYRARLQLQNCLSKK
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800