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The map label for this gene is potD

Identifier: 30995379

GI number: 30995379

Start: 513770

End: 514822

Strand: Reverse

Name: potD

Synonym: HI0498

Alternate gene names: 30995379

Gene position: 514822-513770 (Counterclockwise)

Preceding gene: 16272452

Following gene: 16272435

Centisome position: 28.13

GC content: 36.85

Gene sequence:

>1053_bases
TTGAAAAAATTCTTTGCTCACTCACTCAAAAATCTTTTCCTTTCCACCACCGCACTTTTTGCGGCTTCCGCTTTTGCCAA
TAACAAACTTTACGTCTATAACTGGACAGACTATGTGCCTTCTGATTTAGTCGCACAGTTCTCTAAAGAAACTGGCATCG
AGGTAATTTATTCTACCTTTGAAAGCAACGAAGAAATGTATGCGAAACTTAAACTTACTCAGAATACTGGTTCTGGCTAT
GATTTGGTGTTTCCATCAAGTTACTACGTAAATAAAATGATTAAGGAAAAGATGCTTCAACCGATTGATCAAAGTAAATT
GACCAATATTCATCAAATCCCTAAACATTTATTAAATAAAGAATTCGACCCAGAAAATAAATATTCATTACCTTACGTTT
ACGGCTTAACTGGTATTGAAGTTAATGCGGATGAGATTGATCCTAAAACCATCACTAGCTGGGCGGATTTATGGAAACCT
GAATTTAAAGGCAAAGTTCTCATGACCAGTGATGCACGTGAAGTGTTCCACGTAGCATTATTACTGGATGGTAAATCGCC
AAATACCACCAATGAAGAAGACATCAAAACAGCTTATGAGCGTTTAGAAAAACTGTTACCAAATGTAGCGACTTTTAACT
CTGATTCTCCTGAAGTCCCTTACGTACAAGGCGAAGTTGCAATTGGTATGATTTGGAATGGTTCAGCTTATTTAGCGCAG
AAAGAAAATCAATCTCTTCAATTTGTGTATCCAAAAGAAGGTGCAATTTTCTGGATGGATAACTATGCGATTCCGACTAC
AGCAAAAAATGTTGAAGGTGCACATAAGTTCATTGACTTTTTACTTCGCCCTGAAAATGCAAAAATTGTTATCGAACGCA
TGGGCTTTTCTATGCCAAATAATGGGGCGAAAACATTGCTAAGCGCGGAAGTTGCTAATGATCCAAAATTATTCCCACCA
GCGGAAGAAGTAGAAAAAGGAATTATGCAAGGCGATGTAGGAGAAGCTGTTGATATTTATGAAAAATATTGGGGCAAATT
AAAAACTAATTAG

Upstream 100 bases:

>100_bases
AAAGGGAAATATGTCAAACCTTTTAGAAAAAAATGCTATACTGCGCGCGCGCAAAATTTGCGTGTTTTATGATGAACGAT
AACCCTCGGAGGTCAATAAA

Downstream 100 bases:

>100_bases
TTTCTCTATCCTGAAAACAAAAAAGTGCGGTAAAAATTTACCGCACTTTTATATGTAAAATTTTTATCTTTAAAGAATAA
AACGGCTCAAATCTTCATTT

Product: spermidine/putrescine ABC transporter periplasmic-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP

Number of amino acids: Translated: 350; Mature: 350

Protein sequence:

>350_residues
MKKFFAHSLKNLFLSTTALFAASAFANNKLYVYNWTDYVPSDLVAQFSKETGIEVIYSTFESNEEMYAKLKLTQNTGSGY
DLVFPSSYYVNKMIKEKMLQPIDQSKLTNIHQIPKHLLNKEFDPENKYSLPYVYGLTGIEVNADEIDPKTITSWADLWKP
EFKGKVLMTSDAREVFHVALLLDGKSPNTTNEEDIKTAYERLEKLLPNVATFNSDSPEVPYVQGEVAIGMIWNGSAYLAQ
KENQSLQFVYPKEGAIFWMDNYAIPTTAKNVEGAHKFIDFLLRPENAKIVIERMGFSMPNNGAKTLLSAEVANDPKLFPP
AEEVEKGIMQGDVGEAVDIYEKYWGKLKTN

Sequences:

>Translated_350_residues
MKKFFAHSLKNLFLSTTALFAASAFANNKLYVYNWTDYVPSDLVAQFSKETGIEVIYSTFESNEEMYAKLKLTQNTGSGY
DLVFPSSYYVNKMIKEKMLQPIDQSKLTNIHQIPKHLLNKEFDPENKYSLPYVYGLTGIEVNADEIDPKTITSWADLWKP
EFKGKVLMTSDAREVFHVALLLDGKSPNTTNEEDIKTAYERLEKLLPNVATFNSDSPEVPYVQGEVAIGMIWNGSAYLAQ
KENQSLQFVYPKEGAIFWMDNYAIPTTAKNVEGAHKFIDFLLRPENAKIVIERMGFSMPNNGAKTLLSAEVANDPKLFPP
AEEVEKGIMQGDVGEAVDIYEKYWGKLKTN
>Mature_350_residues
MKKFFAHSLKNLFLSTTALFAASAFANNKLYVYNWTDYVPSDLVAQFSKETGIEVIYSTFESNEEMYAKLKLTQNTGSGY
DLVFPSSYYVNKMIKEKMLQPIDQSKLTNIHQIPKHLLNKEFDPENKYSLPYVYGLTGIEVNADEIDPKTITSWADLWKP
EFKGKVLMTSDAREVFHVALLLDGKSPNTTNEEDIKTAYERLEKLLPNVATFNSDSPEVPYVQGEVAIGMIWNGSAYLAQ
KENQSLQFVYPKEGAIFWMDNYAIPTTAKNVEGAHKFIDFLLRPENAKIVIERMGFSMPNNGAKTLLSAEVANDPKLFPP
AEEVEKGIMQGDVGEAVDIYEKYWGKLKTN

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family

Homologues:

Organism=Escherichia coli, GI1787367, Length=348, Percent_Identity=57.183908045977, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1787078, Length=360, Percent_Identity=33.0555555555556, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): POTD2_HAEIN (P44731)

Other databases:

- EMBL:   L42023
- PIR:   E64072
- RefSeq:   NP_438656.2
- ProteinModelPortal:   P44731
- SMR:   P44731
- GeneID:   950055
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0498
- NMPDR:   fig|71421.1.peg.469
- TIGR:   HI_0498
- HOGENOM:   HBG638705
- OMA:   AYPLLPK
- ProtClustDB:   CLSK870474
- BioCyc:   HINF71421:HI_0498-MONOMER
- InterPro:   IPR006059
- InterPro:   IPR001188
- PRINTS:   PR00909

Pfam domain/function: PF01547 SBP_bac_1

EC number: NA

Molecular weight: Translated: 39647; Mature: 39647

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKFFAHSLKNLFLSTTALFAASAFANNKLYVYNWTDYVPSDLVAQFSKETGIEVIYSTF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEHHH
ESNEEMYAKLKLTQNTGSGYDLVFPSSYYVNKMIKEKMLQPIDQSKLTNIHQIPKHLLNK
HCCCEEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
EFDPENKYSLPYVYGLTGIEVNADEIDPKTITSWADLWKPEFKGKVLMTSDAREVFHVAL
CCCCCCCCCCCEEEECEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHEEEEEE
LLDGKSPNTTNEEDIKTAYERLEKLLPNVATFNSDSPEVPYVQGEVAIGMIWNGSAYLAQ
EEECCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEECCEEEEEEECCCEEEEE
KENQSLQFVYPKEGAIFWMDNYAIPTTAKNVEGAHKFIDFLLRPENAKIVIERMGFSMPN
CCCCCEEEEECCCCEEEEECCEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCC
NGAKTLLSAEVANDPKLFPPAEEVEKGIMQGDVGEAVDIYEKYWGKLKTN
CCHHHEEEHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKFFAHSLKNLFLSTTALFAASAFANNKLYVYNWTDYVPSDLVAQFSKETGIEVIYSTF
CCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEHHH
ESNEEMYAKLKLTQNTGSGYDLVFPSSYYVNKMIKEKMLQPIDQSKLTNIHQIPKHLLNK
HCCCEEEEEEEEEECCCCCEEEECCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
EFDPENKYSLPYVYGLTGIEVNADEIDPKTITSWADLWKPEFKGKVLMTSDAREVFHVAL
CCCCCCCCCCCEEEECEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCHHHEEEEEE
LLDGKSPNTTNEEDIKTAYERLEKLLPNVATFNSDSPEVPYVQGEVAIGMIWNGSAYLAQ
EEECCCCCCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCEEECCEEEEEEECCCEEEEE
KENQSLQFVYPKEGAIFWMDNYAIPTTAKNVEGAHKFIDFLLRPENAKIVIERMGFSMPN
CCCCCEEEEECCCCEEEEECCEECCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCC
NGAKTLLSAEVANDPKLFPPAEEVEKGIMQGDVGEAVDIYEKYWGKLKTN
CCHHHEEEHHHCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800