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The map label for this gene is grpE [H]

Identifier: 30995352

GI number: 30995352

Start: 78763

End: 79455

Strand: Direct

Name: grpE [H]

Synonym: HI0071

Alternate gene names: 30995352

Gene position: 78763-79455 (Clockwise)

Preceding gene: 16272043

Following gene: 16272049

Centisome position: 4.3

GC content: 38.96

Gene sequence:

>693_bases
ATGACCGCACTTGAATTTAACATTTTCGTCATTATATCGTTTTTTGCTACACTATGCCCAATTACGATTAATCAATTTTG
TTTTACGGAGAAAAAAATGTCAGAACAAGAACAAAAAGTTGAAATCCCAGAAGTAGAAAAGCAAGAAGAAGTCGTTGTGG
AAGAAACACAACAAGCAGAGCATTCTCAAGAATTTGATCCTTTAGAAGAAGCAATTGCTCGCGTGCAAGAGCTTGAAGAG
CAGCTAAAAACTCAAATAGAAGAAGCGGCAAACAAAGAACAGGATATTTTACTTCGCTCTCGTGCTGAAATTGAAAATCT
TCGTCGTCGTACTGAACAAGACGTAGAAAAAGCACATAAATTCGCCTTGGAAAAATTTTCAAAAGACATTTTGAATACTA
TCGATAACCTAGAGCGTGCCCTTGCCACTCCAGCAAATAAAGAAGATGAAAGCGTAAAAGCCTTATTTGACGGTGTAGAA
CTAACCTTAAAAGAATTAGTTTCAACCGTTGGGCGTTTTGGCGTGGAAGCTGTGGGAGTTGTCGGTGAAGCATTTAATCC
TGATTTACATCAAGCCATTTCAATGCAACCAGCTGAAGGCTTTGAAACCAATCAAATTAGCGTCGTATTGCAAAAAGGCT
ACACCTTAAATGGTCGAGTCATTCGCCCAGCGATGGTAATGGTGGCAGCTTAA

Upstream 100 bases:

>100_bases
ACCAATGAAATAAGTTTTTGTGCATTTGTAAGTTAATATCATTGCGAGGCTTACCTACAAGAGCAATGGTTTTAAAGGAA
CGATATAAATGATTCATAAA

Downstream 100 bases:

>100_bases
TTTACATTTTGTCTAAAGAGCGGTCGGTTTTTCGACCGTTTTTTCTTTTTCCTGAATAACTACATAAACTTTACGGATAA
TTTCCCTAAAATCTTCGCTC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTALEFNIFVIISFFATLCPITINQFCFTEKKMSEQEQKVEIPEVEKQEEVVVEETQQAEHSQEFDPLEEAIARVQELEE
QLKTQIEEAANKEQDILLRSRAEIENLRRRTEQDVEKAHKFALEKFSKDILNTIDNLERALATPANKEDESVKALFDGVE
LTLKELVSTVGRFGVEAVGVVGEAFNPDLHQAISMQPAEGFETNQISVVLQKGYTLNGRVIRPAMVMVAA

Sequences:

>Translated_230_residues
MTALEFNIFVIISFFATLCPITINQFCFTEKKMSEQEQKVEIPEVEKQEEVVVEETQQAEHSQEFDPLEEAIARVQELEE
QLKTQIEEAANKEQDILLRSRAEIENLRRRTEQDVEKAHKFALEKFSKDILNTIDNLERALATPANKEDESVKALFDGVE
LTLKELVSTVGRFGVEAVGVVGEAFNPDLHQAISMQPAEGFETNQISVVLQKGYTLNGRVIRPAMVMVAA
>Mature_229_residues
TALEFNIFVIISFFATLCPITINQFCFTEKKMSEQEQKVEIPEVEKQEEVVVEETQQAEHSQEFDPLEEAIARVQELEEQ
LKTQIEEAANKEQDILLRSRAEIENLRRRTEQDVEKAHKFALEKFSKDILNTIDNLERALATPANKEDESVKALFDGVEL
TLKELVSTVGRFGVEAVGVVGEAFNPDLHQAISMQPAEGFETNQISVVLQKGYTLNGRVIRPAMVMVAA

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Homo sapiens, GI24308295, Length=203, Percent_Identity=31.0344827586207, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI40255109, Length=169, Percent_Identity=31.9526627218935, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1788967, Length=203, Percent_Identity=42.8571428571429, Blast_Score=158, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17552458, Length=143, Percent_Identity=34.965034965035, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6324806, Length=180, Percent_Identity=36.6666666666667, Blast_Score=103, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653432, Length=151, Percent_Identity=33.7748344370861, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 26012; Mature: 25881

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALEFNIFVIISFFATLCPITINQFCFTEKKMSEQEQKVEIPEVEKQEEVVVEETQQAE
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
HSQEFDPLEEAIARVQELEEQLKTQIEEAANKEQDILLRSRAEIENLRRRTEQDVEKAHK
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FALEKFSKDILNTIDNLERALATPANKEDESVKALFDGVELTLKELVSTVGRFGVEAVGV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VGEAFNPDLHQAISMQPAEGFETNQISVVLQKGYTLNGRVIRPAMVMVAA
HHCCCCHHHHHHHCCCCCCCCCCCHHEEEEECCCCCCCEEEHHHHHHHCC
>Mature Secondary Structure 
TALEFNIFVIISFFATLCPITINQFCFTEKKMSEQEQKVEIPEVEKQEEVVVEETQQAE
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
HSQEFDPLEEAIARVQELEEQLKTQIEEAANKEQDILLRSRAEIENLRRRTEQDVEKAHK
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FALEKFSKDILNTIDNLERALATPANKEDESVKALFDGVELTLKELVSTVGRFGVEAVGV
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHH
VGEAFNPDLHQAISMQPAEGFETNQISVVLQKGYTLNGRVIRPAMVMVAA
HHCCCCHHHHHHHCCCCCCCCCCCHHEEEEECCCCCCCEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]