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The map label for this gene is mnhD [H]

Identifier: 308535258

GI number: 308535258

Start: 1937539

End: 1939347

Strand: Reverse

Name: mnhD [H]

Synonym: NA

Alternate gene names: 308535258

Gene position: 1939347-1937539 (Counterclockwise)

Preceding gene: 197118061

Following gene: 197118059

Centisome position: 42.02

GC content: 64.84

Gene sequence:

>1809_bases
GTGGAACTCGCCCCCTTTCCCGTTCTGGCTCCGCTTTTTTTGGCGGCGGTTTTGGCGGCGCTGGAGCATGCGACCAACCG
GCACCGGATCCTCGACCTCCTCTCTTTGGCCACCGTCGCGGCCGTGCTGGTCCTGGACCTGCTCCTTTTGAAACGCTCCC
TCTCCGGCACCGTAGTCTACTGGTTCGGCGCCTGGACTCCGATGCAAGGCTTTCCCGTAGGGATCGCCTTCGTCGTGGAT
CCGGTCGCCGCCCTCCTCGCCCTTTTCGCGGCTTTTCTCACCGCCATGGGATTTCTCTATTCCTGGCACCGTTTCAAGGC
GATCGGCACCTACTATCACACGCTCATGCTCCTTTTCCTCGCCTCCATGCAGGGCTTCTGCCTCACCGGCGACCTCTTCA
ACATGTTCGTCTTCTTCGAGATGATGGGGGTCACCGCCTATGCGCTCACCGGGTACAAGATCGAGGACAGCGGCCCCTTG
GAAGGAGCGCTCAACTTCGCCGTGATGAACAGCATCGGCGGCTTGATGGTCCTCTTCGGGATCGGCCTCCTTTACGGCAT
CACCGGCGGGCTCAACCTCGCCTGGGCCGGCGCGTTCCTTGAGGGAAAAGCGGGAGGGGCAACTGTCGCTGCGGCCTTCC
TACTCTTAAGCCTGGGGTTCCTGGTGAAGGGGGCCATCGTCCCCTTTCATTTCTGGCTTCCCGACGCCCATGCCGTCGCC
CCTACCCCCGCCTCCGTCCTTTTCTCCGGCATCATGGTGCAGCTGGGGCTCTACGCGGTGGCGCGCATCTACTGGGTCGT
CTTCAGCGACGCGCTCGACCCCGCCCCCTTGCGGCTCCTGTTGCTGTCTGCTGGTGCCGCTACGGCCGTTTTGGGAGCCG
TCATGGCACTCCTGCAACGGCACCTGAAACGGATGCTCGCCTTCTCCACGGTAAGCCACAGCGGGATGATGCTGACCGGG
ATCGCCCTCATGGACCCGTCCGCCCTCGCCGGGACCCTCCTCTACCTGGTCGGGCACGGGGCGGTCAAGGGGGCCCTCTT
CGTCTGCACCGGGATCATCCTCTATCACTACAATTCGGTGGACGAGGAGACCCTGCGCGGCAAGTGCCTCCCCCTGAAGG
CGACCGGCGCCCTCTTCTGCCTTTGCGGCGTAGCTCTCGCCGGGCTCCCCCCCTTCGGGACCTGGGCCGGAAAGGGAGTG
CTGGAGCAGAGCGCCGAGAGCCTCGGCTACGGGTGGCTCCCCCCCCTGTTCCTGATCTGCTCAGCCATCACGGGCGGGGT
GGTGCTGCGCGCCGGGGGAAGCCTTTTCTACGGCGTAGGCTCTGCCAACTTCCTCTCCAGTTCGGCCCCGTCCTCGGGTA
AAAAGGAGGAACCGGAGGCTTTCGAGGAGAAAGGGGCGACACCGAGGATGATGCTCCTTCCCCTGGTCCTATTGATTGTG
GTCGCAGTCGGGGCCGGGGCCTTGCCGCAGGTGCAGAGTTCCCTTGCAGAAGGAGCAGCGAGGCTCGCGGATGGGAAAGG
GTACCGGGCTACGGTGCTAAAGGGCGGGGCTTACGCTGCGGCAGCGCTGCCGCAACCCGACCGACCGGATTTGGCAATGG
GAGTGGCTGCGGCCCTCGCGGCGCTAGCCGTGGCGGGAATTTTTCTGGCCCCGCCGGACGCGGTGCGGCCGCTGAGGGGC
CTGCGGCGCGCGCTCGAAGGACCGGTAGCGCTCTTAAGATCCCTGCACAGCGGCTACATCGGGGACTACACCGCCTGGTT
TCTGGCAGGGGCCGCAGTCATGACGCTTTTCGCCATATTGTCGCAATGA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: monovalent cation/proton antiporter subunit MnhD

Products: H2; oxidized ferredoxin [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 602; Mature: 602

Protein sequence:
NA

Sequences:

>Translated_602_residues
MELAPFPVLAPLFLAAVLAALEHATNRHRILDLLSLATVAAVLVLDLLLLKRSLSGTVVYWFGAWTPMQGFPVGIAFVVD
PVAALLALFAAFLTAMGFLYSWHRFKAIGTYYHTLMLLFLASMQGFCLTGDLFNMFVFFEMMGVTAYALTGYKIEDSGPL
EGALNFAVMNSIGGLMVLFGIGLLYGITGGLNLAWAGAFLEGKAGGATVAAAFLLLSLGFLVKGAIVPFHFWLPDAHAVA
PTPASVLFSGIMVQLGLYAVARIYWVVFSDALDPAPLRLLLLSAGAATAVLGAVMALLQRHLKRMLAFSTVSHSGMMLTG
IALMDPSALAGTLLYLVGHGAVKGALFVCTGIILYHYNSVDEETLRGKCLPLKATGALFCLCGVALAGLPPFGTWAGKGV
LEQSAESLGYGWLPPLFLICSAITGGVVLRAGGSLFYGVGSANFLSSSAPSSGKKEEPEAFEEKGATPRMMLLPLVLLIV
VAVGAGALPQVQSSLAEGAARLADGKGYRATVLKGGAYAAAALPQPDRPDLAMGVAAALAALAVAGIFLAPPDAVRPLRG
LRRALEGPVALLRSLHSGYIGDYTAWFLAGAAVMTLFAILSQ
>Mature_602_residues
MELAPFPVLAPLFLAAVLAALEHATNRHRILDLLSLATVAAVLVLDLLLLKRSLSGTVVYWFGAWTPMQGFPVGIAFVVD
PVAALLALFAAFLTAMGFLYSWHRFKAIGTYYHTLMLLFLASMQGFCLTGDLFNMFVFFEMMGVTAYALTGYKIEDSGPL
EGALNFAVMNSIGGLMVLFGIGLLYGITGGLNLAWAGAFLEGKAGGATVAAAFLLLSLGFLVKGAIVPFHFWLPDAHAVA
PTPASVLFSGIMVQLGLYAVARIYWVVFSDALDPAPLRLLLLSAGAATAVLGAVMALLQRHLKRMLAFSTVSHSGMMLTG
IALMDPSALAGTLLYLVGHGAVKGALFVCTGIILYHYNSVDEETLRGKCLPLKATGALFCLCGVALAGLPPFGTWAGKGV
LEQSAESLGYGWLPPLFLICSAITGGVVLRAGGSLFYGVGSANFLSSSAPSSGKKEEPEAFEEKGATPRMMLLPLVLLIV
VAVGAGALPQVQSSLAEGAARLADGKGYRATVLKGGAYAAAALPQPDRPDLAMGVAAALAALAVAGIFLAPPDAVRPLRG
LRRALEGPVALLRSLHSGYIGDYTAWFLAGAAVMTLFAILSQ

Specific function: Mrp complex is a Na(+)/H(+) antiporter that is considered to be the major Na(+) excretion system in B.subtilis. Has a major role in Na(+) resistance and a minor role in Na(+)- and K(+)-dependent pH homeostasis as compared to TetB. MrpA may be the actual N

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=387, Percent_Identity=27.1317829457364, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI251831116, Length=221, Percent_Identity=23.0769230769231, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788827, Length=388, Percent_Identity=28.0927835051546, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1788613, Length=342, Percent_Identity=28.6549707602339, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788831, Length=289, Percent_Identity=29.4117647058824, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1788829, Length=336, Percent_Identity=30.3571428571429, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693160, Length=384, Percent_Identity=23.6979166666667, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1788614, Length=456, Percent_Identity=26.9736842105263, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI2367154, Length=407, Percent_Identity=29.4840294840295, Blast_Score=79, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 62876; Mature: 62876

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELAPFPVLAPLFLAAVLAALEHATNRHRILDLLSLATVAAVLVLDLLLLKRSLSGTVVY
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
WFGAWTPMQGFPVGIAFVVDPVAALLALFAAFLTAMGFLYSWHRFKAIGTYYHTLMLLFL
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASMQGFCLTGDLFNMFVFFEMMGVTAYALTGYKIEDSGPLEGALNFAVMNSIGGLMVLFG
HHCCCCEEEHHHHHHHHHHHHHHHHHHHEECEEECCCCCCHHHHHHHHHHHHHHHHHHHH
IGLLYGITGGLNLAWAGAFLEGKAGGATVAAAFLLLSLGFLVKGAIVPFHFWLPDAHAVA
HHHHHHHCCCCCHHEECHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCC
PTPASVLFSGIMVQLGLYAVARIYWVVFSDALDPAPLRLLLLSAGAATAVLGAVMALLQR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
HLKRMLAFSTVSHSGMMLTGIALMDPSALAGTLLYLVGHGAVKGALFVCTGIILYHYNSV
HHHHHHHHHHHCCCCEEEEEEHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC
DEETLRGKCLPLKATGALFCLCGVALAGLPPFGTWAGKGVLEQSAESLGYGWLPPLFLIC
CHHHHCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
SAITGGVVLRAGGSLFYGVGSANFLSSSAPSSGKKEEPEAFEEKGATPRMMLLPLVLLIV
HHHHCCEEEEECCCEEEECCCCHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHH
VAVGAGALPQVQSSLAEGAARLADGKGYRATVLKGGAYAAAALPQPDRPDLAMGVAAALA
HHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEECCCCCCCCCHHHHHHHHHHH
ALAVAGIFLAPPDAVRPLRGLRRALEGPVALLRSLHSGYIGDYTAWFLAGAAVMTLFAIL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SQ
CC
>Mature Secondary Structure
MELAPFPVLAPLFLAAVLAALEHATNRHRILDLLSLATVAAVLVLDLLLLKRSLSGTVVY
CCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
WFGAWTPMQGFPVGIAFVVDPVAALLALFAAFLTAMGFLYSWHRFKAIGTYYHTLMLLFL
EEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASMQGFCLTGDLFNMFVFFEMMGVTAYALTGYKIEDSGPLEGALNFAVMNSIGGLMVLFG
HHCCCCEEEHHHHHHHHHHHHHHHHHHHEECEEECCCCCCHHHHHHHHHHHHHHHHHHHH
IGLLYGITGGLNLAWAGAFLEGKAGGATVAAAFLLLSLGFLVKGAIVPFHFWLPDAHAVA
HHHHHHHCCCCCHHEECHHHCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEECCCCCCCC
PTPASVLFSGIMVQLGLYAVARIYWVVFSDALDPAPLRLLLLSAGAATAVLGAVMALLQR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHH
HLKRMLAFSTVSHSGMMLTGIALMDPSALAGTLLYLVGHGAVKGALFVCTGIILYHYNSV
HHHHHHHHHHHCCCCEEEEEEHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCC
DEETLRGKCLPLKATGALFCLCGVALAGLPPFGTWAGKGVLEQSAESLGYGWLPPLFLIC
CHHHHCCCCEEECHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHH
SAITGGVVLRAGGSLFYGVGSANFLSSSAPSSGKKEEPEAFEEKGATPRMMLLPLVLLIV
HHHHCCEEEEECCCEEEECCCCHHHCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHH
VAVGAGALPQVQSSLAEGAARLADGKGYRATVLKGGAYAAAALPQPDRPDLAMGVAAALA
HHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEECCCCCCCCCHHHHHHHHHHH
ALAVAGIFLAPPDAVRPLRGLRRALEGPVALLRSLHSGYIGDYTAWFLAGAAVMTLFAIL
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
SQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 10568751; 10198001; 11356194 [H]