Click here to switch to the map view.

The map label for this gene is prfA

Identifier: 30265352

GI number: 30265352

Start: 5056436

End: 5057503

Strand: Reverse

Name: prfA

Synonym: BA_5572

Alternate gene names: 30265352

Gene position: 5057503-5056436 (Counterclockwise)

Preceding gene: 30265353

Following gene: 30265351

Centisome position: 96.75

GC content: 40.07

Gene sequence:

>1068_bases
GTGTTAGATCGTTTGCAAGCTGTAGAAAATCGTTATGAGAAGTTAAATGAATTGCTAAGCGACCCAGCAATTATTAGTGA
TTCAAATAAGCTTCGTGAATATTCAAAGGAACAGTCTGATATACAGGAAACGGTAGAGGTGTATCGTGAGTATAAGGATG
TTCGTGAGCAATTAAAAGATGCGAAAGCAATGTTAGAAGATAAGTTAGACGCAGAAATGCGTGAAATGGTAAAAGAAGAG
GTTTCTGAGCTAGAATCACAAGAAAAAACATTATCAGAGCGTCTGAAAATTTTACTTGTACCAAAAGATCCTAACGATGA
TAAGAACGTTATCGTTGAGGTTCGTGGAGCTGCCGGTGGTGACGAGGCTGCTTTATTTGCTGGTGATTTATACCGTATGT
ATAGCCGTTACGCTGAGGTACAAGGTTGGAAAACGGAGATTATCGAGGCTAGCTATACAGAGTTAGGTGGATATAAAGAG
ATTATCTTTATGATTAACGGTAAAGGTGCTTTCGCGAAGTTGAAATTTGAGAATGGCGCTCACCGTGTACAACGTGTTCC
TGAAACGGAATCTGGTGGACGTATTCATACATCTACAGCAACTGTAGCTGTATTACCAGAGGCAGAAGAAGTAGAAATTG
ATATTCATGAGAAAGATGTTCGTGTTGATACATTCGCTTCTAGTGGACCTGGTGGACAGAGCGTTAATACAACGATGTCA
GCGGTACGTTTAACGCATTTACCGACTGGTGTAGTTGTATCGTGTCAGGATGAGAAATCACAAATTAAGAATAAAGAAAA
AGCGATGAAAGTATTACGCGCACGTGTTTATGATAAGTTTAGACAAGAAGCACAAGCTGAGTATGATCAAAACCGTAAAC
AAGCTGTTGGTACGGGTGATCGTTCAGAGCGTATTCGTACGTATAACTTCCCGCAAAACCGTGTTACAGACCATCGAATC
GGTTTAACGATTCAAAAGCTAGATCAAATCTTACAAGGTAAGTTAGATGATTTCATCAATGCCTTAGTGATGGAAGATCA
GGCTCAAAGGATGGAGGCAGCTGAGTAA

Upstream 100 bases:

>100_bases
AGCTGAACGAGCCACCGCCACTTTTAAATATAATTTGCGTTTTTTTGAGTAAGTACCATATACTATTTGTATTAGATACT
AGGATGTAGAGGTGAATGAT

Downstream 100 bases:

>100_bases
TGCGTGTCTATGAAGCCCTGAAATGGGCTTCTTCTTTTTTACAGGAGAATGGGCGAGATGAAAATGCGGGAGAAATTGTC
CTTTGTCATGTATTAAAGAC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MLDRLQAVENRYEKLNELLSDPAIISDSNKLREYSKEQSDIQETVEVYREYKDVREQLKDAKAMLEDKLDAEMREMVKEE
VSELESQEKTLSERLKILLVPKDPNDDKNVIVEVRGAAGGDEAALFAGDLYRMYSRYAEVQGWKTEIIEASYTELGGYKE
IIFMINGKGAFAKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNTTMS
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVGTGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQILQGKLDDFINALVMEDQAQRMEAAE

Sequences:

>Translated_355_residues
MLDRLQAVENRYEKLNELLSDPAIISDSNKLREYSKEQSDIQETVEVYREYKDVREQLKDAKAMLEDKLDAEMREMVKEE
VSELESQEKTLSERLKILLVPKDPNDDKNVIVEVRGAAGGDEAALFAGDLYRMYSRYAEVQGWKTEIIEASYTELGGYKE
IIFMINGKGAFAKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNTTMS
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVGTGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQILQGKLDDFINALVMEDQAQRMEAAE
>Mature_355_residues
MLDRLQAVENRYEKLNELLSDPAIISDSNKLREYSKEQSDIQETVEVYREYKDVREQLKDAKAMLEDKLDAEMREMVKEE
VSELESQEKTLSERLKILLVPKDPNDDKNVIVEVRGAAGGDEAALFAGDLYRMYSRYAEVQGWKTEIIEASYTELGGYKE
IIFMINGKGAFAKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNTTMS
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVGTGDRSERIRTYNFPQNRVTDHRI
GLTIQKLDQILQGKLDDFINALVMEDQAQRMEAAE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=288, Percent_Identity=45.1388888888889, Blast_Score=265, Evalue=7e-71,
Organism=Homo sapiens, GI34577120, Length=342, Percent_Identity=37.1345029239766, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI166795305, Length=207, Percent_Identity=40.5797101449275, Blast_Score=171, Evalue=1e-42,
Organism=Escherichia coli, GI1787462, Length=357, Percent_Identity=51.2605042016807, Blast_Score=364, Evalue=1e-102,
Organism=Escherichia coli, GI2367172, Length=338, Percent_Identity=36.094674556213, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17542784, Length=281, Percent_Identity=38.7900355871886, Blast_Score=191, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6321295, Length=334, Percent_Identity=43.4131736526946, Blast_Score=243, Evalue=3e-65,
Organism=Drosophila melanogaster, GI19921226, Length=302, Percent_Identity=42.7152317880795, Blast_Score=235, Evalue=4e-62,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_BACAN (Q81JX1)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_847729.1
- RefSeq:   YP_022240.1
- RefSeq:   YP_031417.1
- ProteinModelPortal:   Q81JX1
- SMR:   Q81JX1
- EnsemblBacteria:   EBBACT00000012375
- EnsemblBacteria:   EBBACT00000014683
- EnsemblBacteria:   EBBACT00000022198
- GeneID:   1085262
- GeneID:   2819896
- GeneID:   2852042
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_5572
- KEGG:   bar:GBAA_5572
- KEGG:   bat:BAS5178
- TIGR:   BA_5572
- TIGR:   GBAA_5572
- GeneTree:   EBGT00050000000673
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- ProtClustDB:   PRK00591
- BioCyc:   BANT260799:BAS5178-MONOMER
- BioCyc:   BANT261594:GBAA5572-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40357; Mature: 40357

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRLQAVENRYEKLNELLSDPAIISDSNKLREYSKEQSDIQETVEVYREYKDVREQLKD
CCHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAMLEDKLDAEMREMVKEEVSELESQEKTLSERLKILLVPKDPNDDKNVIVEVRGAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCC
DEAALFAGDLYRMYSRYAEVQGWKTEIIEASYTELGGYKEIIFMINGKGAFAKLKFENGA
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCH
HRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEECCCCCEEEEEEECCCCEEEECCCCCCCCCCHHHHHH
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVGTGD
HHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTIQKLDQILQGKLDDFINALVMEDQAQRMEAAE
CCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDRLQAVENRYEKLNELLSDPAIISDSNKLREYSKEQSDIQETVEVYREYKDVREQLKD
CCHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AKAMLEDKLDAEMREMVKEEVSELESQEKTLSERLKILLVPKDPNDDKNVIVEVRGAAGG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCEEEEEEECCCCC
DEAALFAGDLYRMYSRYAEVQGWKTEIIEASYTELGGYKEIIFMINGKGAFAKLKFENGA
CCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEEECCCCEEEEEECCCH
HRVQRVPETESGGRIHTSTATVAVLPEAEEVEIDIHEKDVRVDTFASSGPGGQSVNTTMS
HHHHCCCCCCCCCEEEECCEEEEECCCCCEEEEEEECCCCEEEECCCCCCCCCCHHHHHH
AVRLTHLPTGVVVSCQDEKSQIKNKEKAMKVLRARVYDKFRQEAQAEYDQNRKQAVGTGD
HHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
RSERIRTYNFPQNRVTDHRIGLTIQKLDQILQGKLDDFINALVMEDQAQRMEAAE
CCCCCEECCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12721629