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The map label for this gene is estA [H]

Identifier: 30265134

GI number: 30265134

Start: 4838148

End: 4838888

Strand: Reverse

Name: estA [H]

Synonym: BA_5335

Alternate gene names: 30265134

Gene position: 4838888-4838148 (Counterclockwise)

Preceding gene: 30265135

Following gene: 30265133

Centisome position: 92.57

GC content: 38.6

Gene sequence:

>741_bases
ATGAAATTAGCATCTCCGAAACCATTTACATTTGAGGGTGGAGACCGCGCTGTTTTATTACTACATGGATTCACAGGAAA
CTCAGCTGATGTACGTATGTTAGGGCGTTTCTTAGAAAAGAAAGGCTACACTTGTCATGCGCCAATTTATAAAGGGCACG
GTGTCCCACCAGAAGAGCTTGTTCATACAGGTCCTGAAGATTGGTGGCAAGACGTAACAGAGGCATATCAGCTTTTAAAA
GATAAAGGTTTTGAGAAAATTGCTGTCGTTGGATTGTCACTTGGCGGAGTATTTTCTCTAAAATTAGGTTATACAGTACC
GGTTTTAGGTGTAGTACCAATGTGTGCACCGATGTATATTAAGAGTGAAGAAACGATGTACCAAGGTATATTGGCATATG
CCCGCGAATATAAAAAGCGAGAGCAAAAATCACCAGAGCAAATCGAACAAGAAATGTTGGAATTCCAAAAGACACCGATG
AATACATTAAAAGCATTACAACAATTAATTGCTGACGTACGTAACAATGTGGACATGATTTATGTACCAACATTTGTTGT
ACAAGCGCGTCATGATGAAATGATTAATACAGATAGTGCGAACATTATTTATAACGGTGTAGAATCAACGTTAAAAGACA
TTAAATGGTATGAAGACTCTACGCATGTCATTACACTTGATAAGCAGCGTGACGAGCTACATGAGGATGTATATAACTTC
TTGGAGCAACTAGATTGGTAA

Upstream 100 bases:

>100_bases
TGAAAGATGTTTCTAATTAGATTATAGAAATACAAATATGGTACACTAGATATAGAAAGAATACGACTAGATTACAGAAG
AGAAAGAGGAGAAGTACATG

Downstream 100 bases:

>100_bases
GATCTAGTTGCCTCTTTTTTCATAAGGAATTTTTTGTGAAAACTGTAAAAATCATAAGGGGAGTATTGAAGTTTCGCTTT
ATACATCCCCTATCTTTCGG

Product: carboxylesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MKLASPKPFTFEGGDRAVLLLHGFTGNSADVRMLGRFLEKKGYTCHAPIYKGHGVPPEELVHTGPEDWWQDVTEAYQLLK
DKGFEKIAVVGLSLGGVFSLKLGYTVPVLGVVPMCAPMYIKSEETMYQGILAYAREYKKREQKSPEQIEQEMLEFQKTPM
NTLKALQQLIADVRNNVDMIYVPTFVVQARHDEMINTDSANIIYNGVESTLKDIKWYEDSTHVITLDKQRDELHEDVYNF
LEQLDW

Sequences:

>Translated_246_residues
MKLASPKPFTFEGGDRAVLLLHGFTGNSADVRMLGRFLEKKGYTCHAPIYKGHGVPPEELVHTGPEDWWQDVTEAYQLLK
DKGFEKIAVVGLSLGGVFSLKLGYTVPVLGVVPMCAPMYIKSEETMYQGILAYAREYKKREQKSPEQIEQEMLEFQKTPM
NTLKALQQLIADVRNNVDMIYVPTFVVQARHDEMINTDSANIIYNGVESTLKDIKWYEDSTHVITLDKQRDELHEDVYNF
LEQLDW
>Mature_246_residues
MKLASPKPFTFEGGDRAVLLLHGFTGNSADVRMLGRFLEKKGYTCHAPIYKGHGVPPEELVHTGPEDWWQDVTEAYQLLK
DKGFEKIAVVGLSLGGVFSLKLGYTVPVLGVVPMCAPMYIKSEETMYQGILAYAREYKKREQKSPEQIEQEMLEFQKTPM
NTLKALQQLIADVRNNVDMIYVPTFVVQARHDEMINTDSANIIYNGVESTLKDIKWYEDSTHVITLDKQRDELHEDVYNF
LEQLDW

Specific function: Involved in the detoxification of xenobiotics. Shows maximal activity with C6 substrates, with gradually decreasing activity from C8 to C12 substrates. No activity for higher chain length substrates acids rather than long-chain ones [H]

COG id: COG1647

COG function: function code R; Esterase/lipase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipase/esterase LIP3/BchO family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012354
- InterPro:   IPR001375 [H]

Pfam domain/function: PF00326 Peptidase_S9 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 28153; Mature: 28153

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLASPKPFTFEGGDRAVLLLHGFTGNSADVRMLGRFLEKKGYTCHAPIYKGHGVPPEEL
CCCCCCCCEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHH
VHTGPEDWWQDVTEAYQLLKDKGFEKIAVVGLSLGGVFSLKLGYTVPVLGVVPMCAPMYI
HHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHCCCEE
KSEETMYQGILAYAREYKKREQKSPEQIEQEMLEFQKTPMNTLKALQQLIADVRNNVDMI
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEE
YVPTFVVQARHDEMINTDSANIIYNGVESTLKDIKWYEDSTHVITLDKQRDELHEDVYNF
EECHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHH
LEQLDW
HHHHCC
>Mature Secondary Structure
MKLASPKPFTFEGGDRAVLLLHGFTGNSADVRMLGRFLEKKGYTCHAPIYKGHGVPPEEL
CCCCCCCCEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHH
VHTGPEDWWQDVTEAYQLLKDKGFEKIAVVGLSLGGVFSLKLGYTVPVLGVVPMCAPMYI
HHCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEECCCHHHHHHHHHHHCCCEE
KSEETMYQGILAYAREYKKREQKSPEQIEQEMLEFQKTPMNTLKALQQLIADVRNNVDMI
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEE
YVPTFVVQARHDEMINTDSANIIYNGVESTLKDIKWYEDSTHVITLDKQRDELHEDVYNF
EECHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHH
LEQLDW
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1369099 [H]