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The map label for this gene is racE-2 [H]

Identifier: 30264552

GI number: 30264552

Start: 4290945

End: 4291754

Strand: Reverse

Name: racE-2 [H]

Synonym: BA_4717

Alternate gene names: 30264552

Gene position: 4291754-4290945 (Counterclockwise)

Preceding gene: 30264553

Following gene: 30264551

Centisome position: 82.1

GC content: 38.02

Gene sequence:

>810_bases
ATGAAGTTGAATAGAGCAATCGGTGTTATCGATTCAGGAGTTGGCGGCTTAACAGTAGCGAAGGAATTAATTCGTCAGTT
GCCGAAAGAGCGCATTATATATTTAGGGGATACAGCACGTTGCCCTTATGGTCCACGTTCTCGAGAAGAAGTGCGCCAAT
TCACGTGGGAAATGACGGAGCATTTACTAGATTTAAATATCAAAATGTTAGTTATTGCATGCAATACAGCAACTGCAGTT
GTATTAGAAGAGATGCAGAAACAATTACCAATTCCAGTGGTAGGAGTTATTCACCCAGGATCACGTACAGCTTTAAAAGT
GACAAACACATACCATGTTGGGATTATTGGAACGATTGGAACGGTGAAAAGTGGTGCATACGAAGAGGCGTTAAAGTCTA
TTAATAACCGTGTTATGGTAGAAAGTTTAGCGTGTCCGCCTTTCGTTGAACTTGTAGAGAGTGGCAATTTTGAAAGTGAA
ATGGCATATGAAGTTGTAAGAGAAACGTTGCAACCGCTGAAAAATACTGATATTGATACACTTATTTTAGGTTGTACACA
TTATCCGATTTTAGGTCCTGTCATTAAACAAGTAATGGGAGATAAAGTACAACTCATTAGTTCAGGTGATGAAACAGCGC
GTGAAGTAAGTACAATTTTATACCATAGTAAAATGTTGAATGAGGGAGAGGAGCAAAGCGACCATCTCTTCTTAACAACA
GGGAAAATAGGCTTATTTAAAGAAATTGCATCAAAATGGTTCGGTCAACCGATTGAAAATGTGAAACATATTCATTTAGA
AAAAGAATAA

Upstream 100 bases:

>100_bases
GGTACAAATGAAAATTTTCATACAGAAGCAGTTTAAGTGCGCGAACTTAAAGTGTGTAAAATATAATGATAAAATAAGAT
GATGAATGAGAAAACGGGTG

Downstream 100 bases:

>100_bases
TAGTAGAATCCATATAAGTGAACTCCTGAGAAATCAGGGGTTTTTTGTTTATAGGGAGCACAGCTTGTTCTAAAAAGAGA
AACAGCTCGTATACATAATA

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 269; Mature: 269

Protein sequence:

>269_residues
MKLNRAIGVIDSGVGGLTVAKELIRQLPKERIIYLGDTARCPYGPRSREEVRQFTWEMTEHLLDLNIKMLVIACNTATAV
VLEEMQKQLPIPVVGVIHPGSRTALKVTNTYHVGIIGTIGTVKSGAYEEALKSINNRVMVESLACPPFVELVESGNFESE
MAYEVVRETLQPLKNTDIDTLILGCTHYPILGPVIKQVMGDKVQLISSGDETAREVSTILYHSKMLNEGEEQSDHLFLTT
GKIGLFKEIASKWFGQPIENVKHIHLEKE

Sequences:

>Translated_269_residues
MKLNRAIGVIDSGVGGLTVAKELIRQLPKERIIYLGDTARCPYGPRSREEVRQFTWEMTEHLLDLNIKMLVIACNTATAV
VLEEMQKQLPIPVVGVIHPGSRTALKVTNTYHVGIIGTIGTVKSGAYEEALKSINNRVMVESLACPPFVELVESGNFESE
MAYEVVRETLQPLKNTDIDTLILGCTHYPILGPVIKQVMGDKVQLISSGDETAREVSTILYHSKMLNEGEEQSDHLFLTT
GKIGLFKEIASKWFGQPIENVKHIHLEKE
>Mature_269_residues
MKLNRAIGVIDSGVGGLTVAKELIRQLPKERIIYLGDTARCPYGPRSREEVRQFTWEMTEHLLDLNIKMLVIACNTATAV
VLEEMQKQLPIPVVGVIHPGSRTALKVTNTYHVGIIGTIGTVKSGAYEEALKSINNRVMVESLACPPFVELVESGNFESE
MAYEVVRETLQPLKNTDIDTLILGCTHYPILGPVIKQVMGDKVQLISSGDETAREVSTILYHSKMLNEGEEQSDHLFLTT
GKIGLFKEIASKWFGQPIENVKHIHLEKE

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis [H]

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family [H]

Homologues:

Organism=Escherichia coli, GI87082355, Length=220, Percent_Identity=33.1818181818182, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391 [H]

Pfam domain/function: PF01177 Asp_Glu_race [H]

EC number: =5.1.1.3 [H]

Molecular weight: Translated: 29909; Mature: 29909

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00923 ASP_GLU_RACEMASE_1 ; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNRAIGVIDSGVGGLTVAKELIRQLPKERIIYLGDTARCPYGPRSREEVRQFTWEMTE
CCCCHHHHHHCCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHH
HLLDLNIKMLVIACNTATAVVLEEMQKQLPIPVVGVIHPGSRTALKVTNTYHVGIIGTIG
HHHCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECEEEEEEEECCC
TVKSGAYEEALKSINNRVMVESLACPPFVELVESGNFESEMAYEVVRETLQPLKNTDIDT
HHCCCHHHHHHHHHCCCEEEHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHH
LILGCTHYPILGPVIKQVMGDKVQLISSGDETAREVSTILYHSKMLNEGEEQSDHLFLTT
EEECCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
GKIGLFKEIASKWFGQPIENVKHIHLEKE
CCHHHHHHHHHHHCCCCHHHHHHEEECCC
>Mature Secondary Structure
MKLNRAIGVIDSGVGGLTVAKELIRQLPKERIIYLGDTARCPYGPRSREEVRQFTWEMTE
CCCCHHHHHHCCCCCHHHHHHHHHHHCCHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHH
HLLDLNIKMLVIACNTATAVVLEEMQKQLPIPVVGVIHPGSRTALKVTNTYHVGIIGTIG
HHHCCCEEEEEEEECCHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECEEEEEEEECCC
TVKSGAYEEALKSINNRVMVESLACPPFVELVESGNFESEMAYEVVRETLQPLKNTDIDT
HHCCCHHHHHHHHHCCCEEEHHCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHH
LILGCTHYPILGPVIKQVMGDKVQLISSGDETAREVSTILYHSKMLNEGEEQSDHLFLTT
EEECCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
GKIGLFKEIASKWFGQPIENVKHIHLEKE
CCHHHHHHHHHHHCCCCHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9311114 [H]