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The map label for this gene is pepT-2 [H]

Identifier: 30264221

GI number: 30264221

Start: 3985568

End: 3986686

Strand: Reverse

Name: pepT-2 [H]

Synonym: BA_4368

Alternate gene names: 30264221

Gene position: 3986686-3985568 (Counterclockwise)

Preceding gene: 30264222

Following gene: 30264219

Centisome position: 76.27

GC content: 38.43

Gene sequence:

>1119_bases
ATGATTAATCAAGAACGTTTAGTAAATGAATTCATGGAATTAGTACAAGTAGATTCTGAAACGAAATTTGAAGCAGAAAT
TTGCAAAGTATTAACAAAGAAATTTACAGATTTAGGTGTAGAAGTATTTGAAGATGACACAATGGCTGTTACTGGGCATG
GTGCAGGTAACTTAATTTGTACATTACCAGCAACAAAAGATGGTGTTGATACAATTTACTTTACTTCTCATATGGATACA
GTAGTTCCTGGTAATGGAATTAAGCCTTCTATTAAAGATGGATATATCGTATCAGATGGTACTACGATTTTAGGTGCGGA
TGATAAAGCGGGATTAGCATCAATGTTTGAAGCAATCCGTGTTTTAAAAGAGAAAAATATCCCTCACGGCACAATTGAAT
TTATTATTACAGTTGGAGAAGAATCTGGTCTTGTTGGTGCAAAAGCATTAGATCGTGAGCGCATTACAGCGAAATATGGT
TACGCGTTAGATAGCGATGGGAAAGTTGGCGAAATCGTTGTTGCAGCTCCAACACAAGCGAAAGTGAACGCGATTATTCG
CGGGAAAACAGCTCATGCAGGTGTAGCACCGGAAAAAGGCGTATCTGCAATTACGATCGCAGCGAAAGCAATTGCGAAGA
TGCCACTTGGTCGTATTGATTCTGAAACAACTGCAAATATTGGACGTTTTGAAGGTGGTACACAAACGAATATCGTTTGC
GATCATGTACAAATCTTTGCAGAAGCGCGTTCTTTAATCAATGAAAAAATGGAAGTACAAGTTGCGAAAATGAAAGAAGC
ATTTGAAACAACTGCAAAAGAAATGGGCGGCCAAGCAGATGTTGAAGTAAAGGTTATGTACCCAGGATTTAAATTTGCTG
ATGGGGATCACGTTGTAGAAGTTGCAAAACGCGCAGCTGAAAAAATTGGTCGTACACCTTCTCTTCACCAAAGTGGTGGC
GGAAGTGATGCAAACGTAATTGCTGGACACGGAATTCCAACAGTTAACTTAGCAGTTGGTTATGAAGAAATTCATACAAC
AAACGAAAAGATTCCTGTTGAAGAATTAGCGAAAACAGCAGAATTAGTTGTTGCAATCATAGAGGAAGTAGCGAAATAA

Upstream 100 bases:

>100_bases
TAAACCGTCTGTTTTTTGTCTTGTATTTCGAAACTTTTGCGAAATTCTGTTATAATTAATAGTGTTACATACATAATGGT
AGTGCAGGAGGCGCAGTCTT

Downstream 100 bases:

>100_bases
TATAACAAAAAAGCGTGTAAATCAATTGTGATTTACACGCTTTTTACTTTGATTCGATTGCTTTTACAAGGCGGATAAGC
CAATACAAGAAAATCCATGG

Product: peptidase T

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MINQERLVNEFMELVQVDSETKFEAEICKVLTKKFTDLGVEVFEDDTMAVTGHGAGNLICTLPATKDGVDTIYFTSHMDT
VVPGNGIKPSIKDGYIVSDGTTILGADDKAGLASMFEAIRVLKEKNIPHGTIEFIITVGEESGLVGAKALDRERITAKYG
YALDSDGKVGEIVVAAPTQAKVNAIIRGKTAHAGVAPEKGVSAITIAAKAIAKMPLGRIDSETTANIGRFEGGTQTNIVC
DHVQIFAEARSLINEKMEVQVAKMKEAFETTAKEMGGQADVEVKVMYPGFKFADGDHVVEVAKRAAEKIGRTPSLHQSGG
GSDANVIAGHGIPTVNLAVGYEEIHTTNEKIPVEELAKTAELVVAIIEEVAK

Sequences:

>Translated_372_residues
MINQERLVNEFMELVQVDSETKFEAEICKVLTKKFTDLGVEVFEDDTMAVTGHGAGNLICTLPATKDGVDTIYFTSHMDT
VVPGNGIKPSIKDGYIVSDGTTILGADDKAGLASMFEAIRVLKEKNIPHGTIEFIITVGEESGLVGAKALDRERITAKYG
YALDSDGKVGEIVVAAPTQAKVNAIIRGKTAHAGVAPEKGVSAITIAAKAIAKMPLGRIDSETTANIGRFEGGTQTNIVC
DHVQIFAEARSLINEKMEVQVAKMKEAFETTAKEMGGQADVEVKVMYPGFKFADGDHVVEVAKRAAEKIGRTPSLHQSGG
GSDANVIAGHGIPTVNLAVGYEEIHTTNEKIPVEELAKTAELVVAIIEEVAK
>Mature_372_residues
MINQERLVNEFMELVQVDSETKFEAEICKVLTKKFTDLGVEVFEDDTMAVTGHGAGNLICTLPATKDGVDTIYFTSHMDT
VVPGNGIKPSIKDGYIVSDGTTILGADDKAGLASMFEAIRVLKEKNIPHGTIEFIITVGEESGLVGAKALDRERITAKYG
YALDSDGKVGEIVVAAPTQAKVNAIIRGKTAHAGVAPEKGVSAITIAAKAIAKMPLGRIDSETTANIGRFEGGTQTNIVC
DHVQIFAEARSLINEKMEVQVAKMKEAFETTAKEMGGQADVEVKVMYPGFKFADGDHVVEVAKRAAEKIGRTPSLHQSGG
GSDANVIAGHGIPTVNLAVGYEEIHTTNEKIPVEELAKTAELVVAIIEEVAK

Specific function: Could be a peptidase [H]

COG id: COG2195

COG function: function code E; Di- and tripeptidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI1787372, Length=415, Percent_Identity=26.2650602409639, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR010162
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.4.11.4 [C]

Molecular weight: Translated: 39611; Mature: 39611

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00758 ARGE_DAPE_CPG2_1 ; PS00759 ARGE_DAPE_CPG2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINQERLVNEFMELVQVDSETKFEAEICKVLTKKFTDLGVEVFEDDTMAVTGHGAGNLIC
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCEEE
TLPATKDGVDTIYFTSHMDTVVPGNGIKPSIKDGYIVSDGTTILGADDKAGLASMFEAIR
ECCCCCCCCCEEEEECCCCEECCCCCCCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHH
VLKEKNIPHGTIEFIITVGEESGLVGAKALDRERITAKYGYALDSDGKVGEIVVAAPTQA
HHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCEEECCCCCEEEEEEECCCCC
KVNAIIRGKTAHAGVAPEKGVSAITIAAKAIAKMPLGRIDSETTANIGRFEGGTQTNIVC
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCEEH
DHVQIFAEARSLINEKMEVQVAKMKEAFETTAKEMGGQADVEVKVMYPGFKFADGDHVVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEECCCHHHHH
VAKRAAEKIGRTPSLHQSGGGSDANVIAGHGIPTVNLAVGYEEIHTTNEKIPVEELAKTA
HHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEEECHHHHHCCCCCCCHHHHHHHH
ELVVAIIEEVAK
HHHHHHHHHHCC
>Mature Secondary Structure
MINQERLVNEFMELVQVDSETKFEAEICKVLTKKFTDLGVEVFEDDTMAVTGHGAGNLIC
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEECCCEEEEEECCCCCEEE
TLPATKDGVDTIYFTSHMDTVVPGNGIKPSIKDGYIVSDGTTILGADDKAGLASMFEAIR
ECCCCCCCCCEEEEECCCCEECCCCCCCCCCCCCEEEECCCEEEECCCCCHHHHHHHHHH
VLKEKNIPHGTIEFIITVGEESGLVGAKALDRERITAKYGYALDSDGKVGEIVVAAPTQA
HHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHCCEEECCCCCEEEEEEECCCCC
KVNAIIRGKTAHAGVAPEKGVSAITIAAKAIAKMPLGRIDSETTANIGRFEGGTQTNIVC
EEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCCCCCCCEEH
DHVQIFAEARSLINEKMEVQVAKMKEAFETTAKEMGGQADVEVKVMYPGFKFADGDHVVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCEEECCCHHHHH
VAKRAAEKIGRTPSLHQSGGGSDANVIAGHGIPTVNLAVGYEEIHTTNEKIPVEELAKTA
HHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEEEEECHHHHHCCCCCCCHHHHHHHH
ELVVAIIEEVAK
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Aminopeptidases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]