Click here to switch to the map view.

The map label for this gene is gid

Identifier: 30263836

GI number: 30263836

Start: 3646948

End: 3648252

Strand: Reverse

Name: gid

Synonym: BA_3970

Alternate gene names: 30263836

Gene position: 3648252-3646948 (Counterclockwise)

Preceding gene: 30263837

Following gene: 30263835

Centisome position: 69.79

GC content: 39.92

Gene sequence:

>1305_bases
ATGACAACACAAGTAGTAAACGTCATTGGCGCAGGTCTTGCAGGAAGTGAAGCAGCTTACCAAATTGCAAAACGTGGTGT
CCAAGTAAGATTATACGAAATGAGACCGGTAAGGCAAACGCCAGCTCATCACACAGATAAATTTGCAGAATTAGTATGTA
GTAACTCACTTCGCGCAAACACATTAACAAATGCGGTTGGTGTTATTAAAGAAGAAATGCGTTTAATGGATTCTGTTATT
ATTCGTGCAGCTGATGAGTGCTCTGTACCAGCAGGAGGGGCTTTAGCGGTAGATCGTCATGAATTTGCGGCGAAAGTAAC
GGAATATGTAAAAAACCATCCGAACGTAACTGTGATGAATGAAGAAATCACTGAAATTCCAGAAGGTCCTACTATTATCG
CGACAGGTCCACTTACGTCACCGGATCTTTCTGCACAATTAAAAGAATTAACGGGTGAAGACTACTTTTATTTCTATGAT
GCTGCAGCACCGATTGTTGAGAAAGACAGCATTGATATGAATAAGGTGTATTTAAAATCTCGTTACGATAAAGGTGAAGC
GGCATATTTAAACTGCCCAATGACGGAAGAGGAATTTGACCGTTTTTACGAGGCTTTAATCGCTGCTGAAACAGTACCTT
TAAAAGAATTTGAAAAAGAAATTTTCTTTGAAGGTTGTATGCCAGTAGAAGTTATGGCAAGTAGAGGAAGACAAACACTA
GTATTTGGACCGATGAAACCTGTTGGATTAGAAGATCCGAAAACAGGGAAAACGCCATATGCTGTTGTTCAGTTACGTCA
AGATGATGCAGCGGGAACGTTATATAATATCGTAGGTTTCCAAACGCATTTAAAATGGGGACCACAAAAAGAAGTGTTAC
AGTTGATTCCAGGACTGGAAAACGCAGAGATTGTACGTTATGGTGTAATGCATCGTAATACGTTTATTAATTCACCTAAC
TTGCTTCGTCCAACATACCAATATAAACAACGTGATGATTTATTCTTCGCTGGTCAAATGACTGGAGTAGAGGGATATGT
TGAATCGGCAGCTTCAGGATTGTTAGCGGGTATCAACGCTGCTCGACTTGTAAAAGGTGAAGAGCCGGTTGTATTACCAC
CTGTAACTGCAATGGGAAGTATGGCGAATTATATTACTGCGACAAACGCGAAAAACTTCCAACCAATGAATGCGAACTTC
GGTCTGTTTGCACCGTTAGAGAAGAAAATTAAGAAGAAAGCAGAACGTAATGAAGCCTATGCAACACGCGCTTTAGAAAT
GATTCGAAATTTTGTAAATATTTAG

Upstream 100 bases:

>100_bases
TACAAGTACAATGTATTTCGTGTGATTATGAAGAAGAACAACAAATGTGAGCGTAACTGCTCACATTTTTTTCGGGAAGA
AAAGGAAAGGTGATAAATAT

Downstream 100 bases:

>100_bases
TTAAATTTCTTGCAAAGGCTACTAAGTTGTGATACGATTTAGTAGCCTTTATTGAGGTGAATTGTGTGAATGTGAAGAAA
TTGTTACAATTATTCGTTGG

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MTTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRANTLTNAVGVIKEEMRLMDSVI
IRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMNEEITEIPEGPTIIATGPLTSPDLSAQLKELTGEDYFYFYD
AAAPIVEKDSIDMNKVYLKSRYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTL
VFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLENAEIVRYGVMHRNTFINSPN
LLRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINAARLVKGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANF
GLFAPLEKKIKKKAERNEAYATRALEMIRNFVNI

Sequences:

>Translated_434_residues
MTTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRANTLTNAVGVIKEEMRLMDSVI
IRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMNEEITEIPEGPTIIATGPLTSPDLSAQLKELTGEDYFYFYD
AAAPIVEKDSIDMNKVYLKSRYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTL
VFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLENAEIVRYGVMHRNTFINSPN
LLRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINAARLVKGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANF
GLFAPLEKKIKKKAERNEAYATRALEMIRNFVNI
>Mature_433_residues
TTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRANTLTNAVGVIKEEMRLMDSVII
RAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMNEEITEIPEGPTIIATGPLTSPDLSAQLKELTGEDYFYFYDA
AAPIVEKDSIDMNKVYLKSRYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTLV
FGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLENAEIVRYGVMHRNTFINSPNL
LRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINAARLVKGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANFG
LFAPLEKKIKKKAERNEAYATRALEMIRNFVNI

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Escherichia coli, GI2367273, Length=90, Percent_Identity=45.5555555555556, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17534255, Length=131, Percent_Identity=34.3511450381679, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6321202, Length=87, Percent_Identity=43.6781609195402, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_BACAA (C3P5N4)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868070.1
- ProteinModelPortal:   C3P5N4
- SMR:   C3P5N4
- EnsemblBacteria:   EBBACT00000128604
- GeneID:   7849787
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_3993
- GeneTree:   EBGT00050000001476
- ProtClustDB:   PRK05335
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 48069; Mature: 47937

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRAN
CCCHHHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHH
TLTNAVGVIKEEMRLMDSVIIRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMN
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEC
EEITEIPEGPTIIATGPLTSPDLSAQLKELTGEDYFYFYDAAAPIVEKDSIDMNKVYLKS
CHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCEECCCCCHHHHHHHH
RYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTL
CCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEE
VFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLE
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCC
NAEIVRYGVMHRNTFINSPNLLRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINA
CCCEEEEEHHHCCEECCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCH
ARLVKGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANFGLFAPLEKKIKKKAERNEAY
HHEECCCCCEEECCHHHHHHHHHHEEECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHH
ATRALEMIRNFVNI
HHHHHHHHHHHHCC
>Mature Secondary Structure 
TTQVVNVIGAGLAGSEAAYQIAKRGVQVRLYEMRPVRQTPAHHTDKFAELVCSNSLRAN
CCHHHHHHHCCCCCCHHHHHHHHCCCEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCHH
TLTNAVGVIKEEMRLMDSVIIRAADECSVPAGGALAVDRHEFAAKVTEYVKNHPNVTVMN
HHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEC
EEITEIPEGPTIIATGPLTSPDLSAQLKELTGEDYFYFYDAAAPIVEKDSIDMNKVYLKS
CHHHHCCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEEEECCCCCEECCCCCHHHHHHHH
RYDKGEAAYLNCPMTEEEFDRFYEALIAAETVPLKEFEKEIFFEGCMPVEVMASRGRQTL
CCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHCCCEEE
VFGPMKPVGLEDPKTGKTPYAVVQLRQDDAAGTLYNIVGFQTHLKWGPQKEVLQLIPGLE
EECCCCCCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHCCCCC
NAEIVRYGVMHRNTFINSPNLLRPTYQYKQRDDLFFAGQMTGVEGYVESAASGLLAGINA
CCCEEEEEHHHCCEECCCCCCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCH
ARLVKGEEPVVLPPVTAMGSMANYITATNAKNFQPMNANFGLFAPLEKKIKKKAERNEAY
HHEECCCCCEEECCHHHHHHHHHHEEECCCCCCCCCCCCCCEECHHHHHHHHHHHHHHHH
ATRALEMIRNFVNI
HHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA