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The map label for this gene is miaA [H]

Identifier: 30263717

GI number: 30263717

Start: 3516365

End: 3517309

Strand: Reverse

Name: miaA [H]

Synonym: BA_3843

Alternate gene names: 30263717

Gene position: 3517309-3516365 (Counterclockwise)

Preceding gene: 30263719

Following gene: 30263716

Centisome position: 67.29

GC content: 37.14

Gene sequence:

>945_bases
GTGCAACGTGAAAAAGTTGCTGTCATCATTGGACCAACTGCTGTTGGGAAGACGAAGTTAAGTATTGATCTTGCTAAAGC
GTTGAACGGTGAAATTATAAGTGGAGATTCCATGCAGATTTATCGTACGATGGATATTGGAACTGCAAAGGTGACGAAAG
AAGAGATGGATGGAATTCCACATTATATGGTCGATATAAAAAATCCGGAAGAATCATTTTCAGTTGCCGAGTTTCAAGAA
CGTGTTCGTAAGCATATTCGAGAGATTACAGAGCGTGGTAAATTACCAATTATCGTTGGTGGAACTGGTCTGTATATACA
GTCTGTTTTATTCGATTATCAGTTTACGGATGATGCTGGTGATGCTATATACCGAGAACAAATGGAAAAGTTAGCATTAG
AACGTGGTGTGGAATATGTACATAAGAAATTACAAGAAGTAGACCCAGAAAGCGCTGAGCGTATTCATGCAAATAATGTG
CGTCGTGTCATTAGAGCGTTAGAAATTTTTCACACGTCGGGTGAAAAAATGAGTGATCAACTTGAAAAACAAGAAAACGA
GTTATTATACGATGTTTCCTTAATTGGCTTGACAATGGATCGTGAAATGCTATACGATCGCATTAACTTACGAGTTGATA
TAATGATGGACCAAGGTTTATTAGAAGAAGTAGAAGGGTTGTATAATAGAGGGATACGAGATTGTCAATCTATTCAAGCG
ATTGGGTATAAAGAGATATATGATTATTTTGAGGATCGTGTCTCTTTAGAAGAAGCGGTATCACAATTAAAGACGAATTC
ACGTCGTTATGCAAAACGTCAATTAACGTGGTTCCGTAATAAGATGGATGTCACATGGTTTGATGTTACAGACGGTGAAA
AAACGTCAGAAATTTTACGATACATAGAAGGAAAGCTACAACTAAAGTCGAATAATAGTAAGTAG

Upstream 100 bases:

>100_bases
TAATGAAAGAACTTGCAAACATGCCGTTAAAATTGGTAAGATTGGATATAATCAATAGATGGAGTACATTACATAAAGAG
AGTTGAATGATATGGGAGAA

Downstream 100 bases:

>100_bases
AGAAAAAGAGGAGGATTCGACATGAAGCAATCAATCAATATTCAAGATCAGTTTTTAAATCAACTCCGTAAAGAGAATAC
GTTTGTTACGCTGTACTTAT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIPHYMVDIKNPEESFSVAEFQE
RVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNV
RRVIRALEIFHTSGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQSIQA
IGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQLKSNNSK

Sequences:

>Translated_314_residues
MQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIPHYMVDIKNPEESFSVAEFQE
RVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNV
RRVIRALEIFHTSGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQSIQA
IGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQLKSNNSK
>Mature_314_residues
MQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIPHYMVDIKNPEESFSVAEFQE
RVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAGDAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNV
RRVIRALEIFHTSGEKMSDQLEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQSIQA
IGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILRYIEGKLQLKSNNSK

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=314, Percent_Identity=31.8471337579618, Blast_Score=155, Evalue=4e-38,
Organism=Escherichia coli, GI1790613, Length=302, Percent_Identity=38.4105960264901, Blast_Score=216, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI25144712, Length=306, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6324848, Length=307, Percent_Identity=34.2019543973941, Blast_Score=179, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24649830, Length=334, Percent_Identity=32.0359281437126, Blast_Score=167, Evalue=8e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 36367; Mature: 36367

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIP
CCCCEEEEEECCCCCCCEEHHEEHHHHCCCCEECCCCEEEEEEECCCCHHCCHHHHCCCC
HYMVDIKNPEESFSVAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAG
EEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCEECCCCC
DAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNVRRVIRALEIFHTSGEKMSDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQSIQA
HHHHHHHEEEEEEEHEEEHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHH
IGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
YIEGKLQLKSNNSK
HHCCCEEEECCCCC
>Mature Secondary Structure
MQREKVAVIIGPTAVGKTKLSIDLAKALNGEIISGDSMQIYRTMDIGTAKVTKEEMDGIP
CCCCEEEEEECCCCCCCEEHHEEHHHHCCCCEECCCCEEEEEEECCCCHHCCHHHHCCCC
HYMVDIKNPEESFSVAEFQERVRKHIREITERGKLPIIVGGTGLYIQSVLFDYQFTDDAG
EEEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHCEECCCCC
DAIYREQMEKLALERGVEYVHKKLQEVDPESAERIHANNVRRVIRALEIFHTSGEKMSDQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LEKQENELLYDVSLIGLTMDREMLYDRINLRVDIMMDQGLLEEVEGLYNRGIRDCQSIQA
HHHHHHHEEEEEEEHEEEHHHHHHHHHHCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHH
IGYKEIYDYFEDRVSLEEAVSQLKTNSRRYAKRQLTWFRNKMDVTWFDVTDGEKTSEILR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHH
YIEGKLQLKSNNSK
HHCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA