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The map label for this gene is glcD [H]

Identifier: 30263468

GI number: 30263468

Start: 3277901

End: 3279292

Strand: Reverse

Name: glcD [H]

Synonym: BA_3575

Alternate gene names: 30263468

Gene position: 3279292-3277901 (Counterclockwise)

Preceding gene: 30263469

Following gene: 30263467

Centisome position: 62.73

GC content: 38.43

Gene sequence:

>1392_bases
ATGGAAATAACAGTAGAGCGTTTAGTAAATGGATTAAAGGGTGTGCTTCCCGAAGATCGAGTAGTAATCAATACGACAGT
AAGAGAGTTACATAGTAAAGATGAATCTTATCATGCTAGTAGTTTACCTGATGTAGTAGTTTTTCCGAAAACGACAGAAG
AAGTTAGTACCATAATGAAAATAGCGAGTGAGTACGGAACACCTGTCGTTCCGTTTGGGGTAGGTTCTAGTTTAGAAGGA
CATGTAATCCCTTATGAAAAGGGAATTACAGTTGATTTTTCATTAATGAACAAAATTCTTGAAATAAGAGAGAAAGATTT
TCTGGTGAAAGTACAGCCAGGAGTGACTCGTTCTCAGCTCAATAAAGAATTGAAGAAATACGGTTTGTTTTTTAGTGTAG
ATCCAGGGGCAGATGCAACGTTAGGTGGAATGGCTGCTACAAATGCAAGTGGAACGACAGCGGTGAAGTATGGTGTTATG
CGTGATCAAGTTCGTGATTTAGAAGTCGTTCTTGCTGATGGAGAAGTAATACATACTGGGAATTTAGCTGCGAAGTCGTC
ATCAGGTTACCATTTAAATGGTGTTTTCGTAGGATCAGAAGGTACGCTTGGATGCTTTACAGAGTTAACATTAAAAGTAT
ATGGCATACCAGAGCATGTTATGGCCGCAAGAGCATCGTTTCCGGCTATAAATGATGCAGTTGAAGCTGTTATTAACATA
TTGCAAGCGGGTATTCCGATTGCGAGAATTGAACTCGTTGATGAACTATCTATGAAACAAGTCAATCATTATAGTGAAAC
AAGTTACAGAGAAGAACCTACATTGTTTTTAGAGTTTCACGGTAATGAAGCAGGGTTGAAGCAAGATGTTGAATTTACGA
AGGAAATTGTTTTTGATCATAAATGTAAAGAAGTTGCTTTTGAAACGGAAACGGCAGCGAGAAATAAATTATGGGATGCA
CGGCATAATCTAGCATATGCTTACGTTCATAGCTATCCTGGTAAAAAGCTTATGAGTACAGACGTATGTGTACCTATTTC
AGAATTAGCTGGAGCTATCCAACAAGCGAAAGAGACATTAGAAAAGAATGGTCTTGTTGGTGGTATTCTTGGTCATGTAG
GAGACGGGAATTTCCACGTGCTCTTAATGGTTGATCCAAACGATAAAGAAGAAGTGGAAAAAGCGGATGAAATAAATGAA
AGTATCGTTCTATATGCTCTTAAACGAGGAGGAACATGTACAGGGGAACATGGCGTTGGTATTGGAAAACGGAAGTATCA
GGAAGAAGAACATGGGGCGGCATTATTCGTAATGGAAAAAATAAAGAAAGCTCTTGATCCGCAAAATATTTTGAATCCGA
ATAAGGTGTTTCAATTAAAGGATAGGAGCTGA

Upstream 100 bases:

>100_bases
GTATATGTAAAACTTGAATTATTTGATTTTTCGGATTATTATGTATAAAACACTTAACATATTTTGTAATTCAAATTTAT
AGAGAGTAGGAGAGTGTACG

Downstream 100 bases:

>100_bases
TTGGGTGAAAGTACTAGAAGCGATAAGTAATATAGCTGGAAAGTATTTTGCATTTTGGGTTATTTGTATTGCAACTATTG
CGTATTTTATTCCCGCTCCA

Product: glycolate oxidase, subunit GlcD, putative

Products: pyruvate; ferrocytochrome c

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MEITVERLVNGLKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMKIASEYGTPVVPFGVGSSLEG
HVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLADGEVIHTGNLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPAINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDHKCKEVAFETETAARNKLWDA
RHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETLEKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINE
SIVLYALKRGGTCTGEHGVGIGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLKDRS

Sequences:

>Translated_463_residues
MEITVERLVNGLKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMKIASEYGTPVVPFGVGSSLEG
HVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLADGEVIHTGNLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPAINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDHKCKEVAFETETAARNKLWDA
RHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETLEKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINE
SIVLYALKRGGTCTGEHGVGIGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLKDRS
>Mature_463_residues
MEITVERLVNGLKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMKIASEYGTPVVPFGVGSSLEG
HVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQLNKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVM
RDQVRDLEVVLADGEVIHTGNLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPAINDAVEAVINI
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDHKCKEVAFETETAARNKLWDA
RHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETLEKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINE
SIVLYALKRGGTCTGEHGVGIGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLKDRS

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=454, Percent_Identity=47.7973568281938, Blast_Score=444, Evalue=1e-124,
Organism=Homo sapiens, GI37595754, Length=477, Percent_Identity=45.2830188679245, Blast_Score=434, Evalue=1e-121,
Organism=Homo sapiens, GI119964728, Length=433, Percent_Identity=26.7898383371824, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4501993, Length=219, Percent_Identity=30.5936073059361, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1789351, Length=465, Percent_Identity=29.8924731182796, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1787977, Length=494, Percent_Identity=27.3279352226721, Blast_Score=145, Evalue=8e-36,
Organism=Escherichia coli, GI48994907, Length=492, Percent_Identity=24.390243902439, Blast_Score=108, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71992373, Length=462, Percent_Identity=43.0735930735931, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71992381, Length=462, Percent_Identity=42.2077922077922, Blast_Score=369, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71992392, Length=361, Percent_Identity=47.0914127423823, Blast_Score=335, Evalue=2e-92,
Organism=Caenorhabditis elegans, GI17534361, Length=453, Percent_Identity=26.7108167770419, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17556096, Length=452, Percent_Identity=25.4424778761062, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6320027, Length=474, Percent_Identity=37.9746835443038, Blast_Score=312, Evalue=7e-86,
Organism=Saccharomyces cerevisiae, GI6320764, Length=470, Percent_Identity=25.3191489361702, Blast_Score=134, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6320023, Length=473, Percent_Identity=25.369978858351, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI18921117, Length=431, Percent_Identity=26.2180974477958, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24639277, Length=431, Percent_Identity=26.2180974477958, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24639275, Length=431, Percent_Identity=26.2180974477958, Blast_Score=138, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24653753, Length=219, Percent_Identity=30.5936073059361, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.2.4

Molecular weight: Translated: 50760; Mature: 50760

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEITVERLVNGLKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMK
CCEEHHHHHHHHHCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHH
IASEYGTPVVPFGVGSSLEGHVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQL
HHHHHCCCEEEECCCCCCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCHHHH
NKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVMRDQVRDLEVVLADGEVIHTG
HHHHHHHCCEEEECCCCCCCCCCEEECCCCCCHHEEHHHHHHHHCCEEEEEECCCEEECC
NLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPAINDAVEAVINI
CCEECCCCCEEEEEEEECCCCCEEEEEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHH
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDH
HHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KCKEVAFETETAARNKLWDARHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETL
HHHHHHHCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
EKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINESIVLYALKRGGTCTGEHGVG
HHCCCEEEEHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCC
IGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLKDRS
CCHHHCCHHHCCCCHHHHHHHHHHCCHHHCCCCCCEEEECCCC
>Mature Secondary Structure
MEITVERLVNGLKGVLPEDRVVINTTVRELHSKDESYHASSLPDVVVFPKTTEEVSTIMK
CCEEHHHHHHHHHCCCCCCCEEEEHHHHHHHCCCCCCCCCCCCCEEEECCCHHHHHHHHH
IASEYGTPVVPFGVGSSLEGHVIPYEKGITVDFSLMNKILEIREKDFLVKVQPGVTRSQL
HHHHHCCCEEEECCCCCCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEECCCCCHHHH
NKELKKYGLFFSVDPGADATLGGMAATNASGTTAVKYGVMRDQVRDLEVVLADGEVIHTG
HHHHHHHCCEEEECCCCCCCCCCEEECCCCCCHHEEHHHHHHHHCCEEEEEECCCEEECC
NLAAKSSSGYHLNGVFVGSEGTLGCFTELTLKVYGIPEHVMAARASFPAINDAVEAVINI
CCEECCCCCEEEEEEEECCCCCEEEEEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHH
LQAGIPIARIELVDELSMKQVNHYSETSYREEPTLFLEFHGNEAGLKQDVEFTKEIVFDH
HHCCCCEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCC
KCKEVAFETETAARNKLWDARHNLAYAYVHSYPGKKLMSTDVCVPISELAGAIQQAKETL
HHHHHHHCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHH
EKNGLVGGILGHVGDGNFHVLLMVDPNDKEEVEKADEINESIVLYALKRGGTCTGEHGVG
HHCCCEEEEHHCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCC
IGKRKYQEEEHGAALFVMEKIKKALDPQNILNPNKVFQLKDRS
CCHHHCCHHHCCCCHHHHHHHHHHCCHHHCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; ferricytochrome c

Specific reaction: (R)-lactate + 2 ferricytochrome c = pyruvate + 2 ferrocytochrome c

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]