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The map label for this gene is ywqM [H]

Identifier: 30263442

GI number: 30263442

Start: 3248656

End: 3249549

Strand: Reverse

Name: ywqM [H]

Synonym: BA_3543

Alternate gene names: 30263442

Gene position: 3249549-3248656 (Counterclockwise)

Preceding gene: 30263444

Following gene: 30263441

Centisome position: 62.17

GC content: 35.91

Gene sequence:

>894_bases
ATGGAGATAAAACAATTAATTACCTTTAAAATAGCCGCGGATACTTTGAACTTTACACAAACTGCGAAGAAATTAAACTT
TGCTCAATCGAGCGTAACGGCGCAAATTAAAACGTTAGAAGCTGAGCTAGGTACGCCGTTATTTGAAAGATTAGGAAAAC
GTCTTTTCTTAACTGAAGCAGGGAGGAAGTTTCAATTATATGCTGATAAGATGATTGGACTTAGTAACGAAGCGAAAATG
GCTGTGAAAGATGATGAAGAAATAGCTGGTACGTTAACGATTGGTGCACAAGAAAGTCAGTGTACATATAGGCTTCCATC
TATATTAAAGAGATTTAAAGCACAATTTCCTCAAATAAAACTTATATTTAAACCAGCACATTCTAATAAAGATGCGAAGG
AACAATTAATGGAGGGAAAAGTCGATCTTGCATTTATTTTGGATGAATGTAAAACAGAAGATGCTTTACATGTGGAGCCG
CTTATGAAAGAAGAATTAAAAGTAGTAGCTGCTCCAGGGCATCGTTTACTGGAGCAAACTTCCGTTTCTATAAAAGATTT
AGAGAAGGAAACACTTTTATTAACAGAACTAGGGTGCTCATATCGGACTTTATTTGAGGAGTTATTTCGTGCGAAAGACA
TATATCCAGCAAATAAGATTGAGTTTGTTAGTGTCGAGGCAATTAAACAATGCATCATTGCAGATTTAGGTGTTGCTGTG
TTACCTGCTATAGTAGTAGAAAAAGATATGCGAGAAGGGACGATAAAGGAGTTAGTTTTAAAAGAAACAATTCCTCCGAT
TTATACGCAAATCGCTTGGCATAAAGACAAATGGATGACAGTGCCACTGCAGCAATTTATCGATGTAACAAGAGAGTTTT
TTACAACGGATTAA

Upstream 100 bases:

>100_bases
TTTGTCGATGCTCTTTATCATACAATATAAATATCAATAGACAAAGAGGATTATTTTGATAAGATCAATCGAAAATTTCG
ATTGAAAAGAGGGATGATAA

Downstream 100 bases:

>100_bases
GTGGAGAAATATTTGTTTTCTATAAAAGAAAAAATAGCAATTTTGTATAAAGAACCGATGCTTGCATGTAACAAGTATCG
GTTCTTTTATGTTTGAAATA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MEIKQLITFKIAADTLNFTQTAKKLNFAQSSVTAQIKTLEAELGTPLFERLGKRLFLTEAGRKFQLYADKMIGLSNEAKM
AVKDDEEIAGTLTIGAQESQCTYRLPSILKRFKAQFPQIKLIFKPAHSNKDAKEQLMEGKVDLAFILDECKTEDALHVEP
LMKEELKVVAAPGHRLLEQTSVSIKDLEKETLLLTELGCSYRTLFEELFRAKDIYPANKIEFVSVEAIKQCIIADLGVAV
LPAIVVEKDMREGTIKELVLKETIPPIYTQIAWHKDKWMTVPLQQFIDVTREFFTTD

Sequences:

>Translated_297_residues
MEIKQLITFKIAADTLNFTQTAKKLNFAQSSVTAQIKTLEAELGTPLFERLGKRLFLTEAGRKFQLYADKMIGLSNEAKM
AVKDDEEIAGTLTIGAQESQCTYRLPSILKRFKAQFPQIKLIFKPAHSNKDAKEQLMEGKVDLAFILDECKTEDALHVEP
LMKEELKVVAAPGHRLLEQTSVSIKDLEKETLLLTELGCSYRTLFEELFRAKDIYPANKIEFVSVEAIKQCIIADLGVAV
LPAIVVEKDMREGTIKELVLKETIPPIYTQIAWHKDKWMTVPLQQFIDVTREFFTTD
>Mature_297_residues
MEIKQLITFKIAADTLNFTQTAKKLNFAQSSVTAQIKTLEAELGTPLFERLGKRLFLTEAGRKFQLYADKMIGLSNEAKM
AVKDDEEIAGTLTIGAQESQCTYRLPSILKRFKAQFPQIKLIFKPAHSNKDAKEQLMEGKVDLAFILDECKTEDALHVEP
LMKEELKVVAAPGHRLLEQTSVSIKDLEKETLLLTELGCSYRTLFEELFRAKDIYPANKIEFVSVEAIKQCIIADLGVAV
LPAIVVEKDMREGTIKELVLKETIPPIYTQIAWHKDKWMTVPLQQFIDVTREFFTTD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082132, Length=296, Percent_Identity=28.3783783783784, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787806, Length=289, Percent_Identity=29.4117647058824, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1788481, Length=290, Percent_Identity=27.5862068965517, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790262, Length=267, Percent_Identity=26.9662921348315, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI157672245, Length=293, Percent_Identity=24.9146757679181, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790208, Length=256, Percent_Identity=27.734375, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787530, Length=256, Percent_Identity=25.390625, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788296, Length=244, Percent_Identity=25, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790399, Length=260, Percent_Identity=26.9230769230769, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1787879, Length=250, Percent_Identity=26, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788887, Length=295, Percent_Identity=25.0847457627119, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1788297, Length=250, Percent_Identity=26, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788748, Length=295, Percent_Identity=21.0169491525424, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786401, Length=303, Percent_Identity=24.7524752475248, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788706, Length=257, Percent_Identity=24.9027237354086, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=23.6947791164659, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788626, Length=253, Percent_Identity=25.296442687747, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1789173, Length=253, Percent_Identity=26.8774703557312, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33701; Mature: 33701

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKQLITFKIAADTLNFTQTAKKLNFAQSSVTAQIKTLEAELGTPLFERLGKRLFLTEA
CCHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEECC
GRKFQLYADKMIGLSNEAKMAVKDDEEIAGTLTIGAQESQCTYRLPSILKRFKAQFPQIK
CCEEEEEHHHHHCCCCCCCEEECCCCHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEE
LIFKPAHSNKDAKEQLMEGKVDLAFILDECKTEDALHVEPLMKEELKVVAAPGHRLLEQT
EEEECCCCCCHHHHHHHCCCCHHEEHHHHHCCCCCEECCHHHHHHHEEEECCCHHHHHHH
SVSIKDLEKETLLLTELGCSYRTLFEELFRAKDIYPANKIEFVSVEAIKQCIIADLGVAV
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
LPAIVVEKDMREGTIKELVLKETIPPIYTQIAWHKDKWMTVPLQQFIDVTREFFTTD
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHEECCCCEEECCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEIKQLITFKIAADTLNFTQTAKKLNFAQSSVTAQIKTLEAELGTPLFERLGKRLFLTEA
CCHHHHEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCEEEEECC
GRKFQLYADKMIGLSNEAKMAVKDDEEIAGTLTIGAQESQCTYRLPSILKRFKAQFPQIK
CCEEEEEHHHHHCCCCCCCEEECCCCHHEEEEEECCCCCCCHHHHHHHHHHHHHCCCCEE
LIFKPAHSNKDAKEQLMEGKVDLAFILDECKTEDALHVEPLMKEELKVVAAPGHRLLEQT
EEEECCCCCCHHHHHHHCCCCHHEEHHHHHCCCCCEECCHHHHHHHEEEECCCHHHHHHH
SVSIKDLEKETLLLTELGCSYRTLFEELFRAKDIYPANKIEFVSVEAIKQCIIADLGVAV
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHHH
LPAIVVEKDMREGTIKELVLKETIPPIYTQIAWHKDKWMTVPLQQFIDVTREFFTTD
HHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHEECCCCEEECCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]