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The map label for this gene is hupE [H]

Identifier: 30263225

GI number: 30263225

Start: 3051197

End: 3052225

Strand: Reverse

Name: hupE [H]

Synonym: BA_3314

Alternate gene names: 30263225

Gene position: 3052225-3051197 (Counterclockwise)

Preceding gene: 30263226

Following gene: 30263224

Centisome position: 58.39

GC content: 33.82

Gene sequence:

>1029_bases
ATGAAATTAACTAAAGATTTAGAAAATATAATTTTAGATCATGGAACAGGAGGACTATTGAGTCAGGATTTAATTAGTTC
AATTATTACTGCAAAACTAGAAGATGTTCACCTTGGAAAAATGGAGGATAGTGCCATTCTTGAGGTAAGTAGTAGAAGAT
TGGCGATGACAACAGATTCTTTTGTTATTGATCCTATCTTTTTTGGTGAGGGGAATATAGGTAAAGTAGCAGTTTGTGGA
ACGGTTAATGATTTAGCGGTTAGTGGTGCAAAACCACTTTATCTATCATTGGCATTAGTACTAGAAGAGGGTTTTCCAAT
TAAAGATTTGGAAGAAATATTAGATTCTATAAGAGAGACCGCGAAAGAAGCTGGAGTGTATATTGTTGCTGGTGATACAA
AAGTTGTTAAAAAAGGTGAAGTAGACAAAATCTTTATTAATACAACTGGAATAGGAGTTTTTGAAGGAGATGTAGCACCG
TTTTCGGTAAACTCTATTCAAGAAGGTGACGATATCATTATAACAGGACAACTAGGAAACCATAGTATACATATCCTTTC
TATCAGAGAAGGATTAGGGTTTGAACAGAGAATAAATAGTGATTGTGCACCATTAAATCATATGATTACAGAGTTAAAAA
ATCATTTTGGTGATTCTATACATTGTATGCGAGATATTACAAGAGGCGGGTTAGGTACAGTTTTAAATGAAGTCTCAGAA
ACAATTAATACTGGAATAAAAATACAAGAAAAAGATATTCCTATGTTAGCAGAAACTATTATGGCTGCCGACATGTTAGG
TGTTAATCCAATGTATCTAGCTAATGAGGGCAATGTTTGTATGTTTGTGTCTCCAGAGGTAAGTGAGGAAGTCGTGAGGG
TATTAAAAAATACTAAATATGGTAAAGAAGCTGCGGTAATTGGTAAAGTTACTCAAACAAAAGAAAGACAAGTACTCATG
GAAGCAAAATCAGGTGAATTGAAACTCATTGAGTTATTATATGGGGCAGAATTACCTCGATTATGTTAG

Upstream 100 bases:

>100_bases
GGGGTTGCAATTCCTTAAGTAAATACGTTGTAATATCACTTGTTCTATGTAGCAAGTGATATTATTTTTCAAAAATAAAA
ATGAAAAGGAGTTTTAACAA

Downstream 100 bases:

>100_bases
GAGACAATTTATGAAAAAAGTAATAAATATGTTTATAAAAAAGTATCAAGTTAAAGGCATTGTGCAAGGTGTTGGTTTTA
GACCTTTCGTATATAGGATA

Product: hydrogenase expression/formation protein HypE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MKLTKDLENIILDHGTGGLLSQDLISSIITAKLEDVHLGKMEDSAILEVSSRRLAMTTDSFVIDPIFFGEGNIGKVAVCG
TVNDLAVSGAKPLYLSLALVLEEGFPIKDLEEILDSIRETAKEAGVYIVAGDTKVVKKGEVDKIFINTTGIGVFEGDVAP
FSVNSIQEGDDIIITGQLGNHSIHILSIREGLGFEQRINSDCAPLNHMITELKNHFGDSIHCMRDITRGGLGTVLNEVSE
TINTGIKIQEKDIPMLAETIMAADMLGVNPMYLANEGNVCMFVSPEVSEEVVRVLKNTKYGKEAAVIGKVTQTKERQVLM
EAKSGELKLIELLYGAELPRLC

Sequences:

>Translated_342_residues
MKLTKDLENIILDHGTGGLLSQDLISSIITAKLEDVHLGKMEDSAILEVSSRRLAMTTDSFVIDPIFFGEGNIGKVAVCG
TVNDLAVSGAKPLYLSLALVLEEGFPIKDLEEILDSIRETAKEAGVYIVAGDTKVVKKGEVDKIFINTTGIGVFEGDVAP
FSVNSIQEGDDIIITGQLGNHSIHILSIREGLGFEQRINSDCAPLNHMITELKNHFGDSIHCMRDITRGGLGTVLNEVSE
TINTGIKIQEKDIPMLAETIMAADMLGVNPMYLANEGNVCMFVSPEVSEEVVRVLKNTKYGKEAAVIGKVTQTKERQVLM
EAKSGELKLIELLYGAELPRLC
>Mature_342_residues
MKLTKDLENIILDHGTGGLLSQDLISSIITAKLEDVHLGKMEDSAILEVSSRRLAMTTDSFVIDPIFFGEGNIGKVAVCG
TVNDLAVSGAKPLYLSLALVLEEGFPIKDLEEILDSIRETAKEAGVYIVAGDTKVVKKGEVDKIFINTTGIGVFEGDVAP
FSVNSIQEGDDIIITGQLGNHSIHILSIREGLGFEQRINSDCAPLNHMITELKNHFGDSIHCMRDITRGGLGTVLNEVSE
TINTGIKIQEKDIPMLAETIMAADMLGVNPMYLANEGNVCMFVSPEVSEEVVRVLKNTKYGKEAAVIGKVTQTKERQVLM
EAKSGELKLIELLYGAELPRLC

Specific function: May be involved in the maturation of the nifE hydrogenase [H]

COG id: COG0309

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypE family [H]

Homologues:

Organism=Escherichia coli, GI226510967, Length=341, Percent_Identity=39.5894428152493, Blast_Score=254, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR011854
- InterPro:   IPR016188 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: NA

Molecular weight: Translated: 37101; Mature: 37101

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTKDLENIILDHGTGGLLSQDLISSIITAKLEDVHLGKMEDSAILEVSSRRLAMTTDS
CCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCCEEEEECCC
FVIDPIFFGEGNIGKVAVCGTVNDLAVSGAKPLYLSLALVLEEGFPIKDLEEILDSIRET
EEEEEEEECCCCCCCEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AKEAGVYIVAGDTKVVKKGEVDKIFINTTGIGVFEGDVAPFSVNSIQEGDDIIITGQLGN
HHHCCEEEEECCCEEEECCCCCEEEEEECCCEEEECCCCCEECCCCCCCCCEEEEEECCC
HSIHILSIREGLGFEQRINSDCAPLNHMITELKNHFGDSIHCMRDITRGGLGTVLNEVSE
CEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHH
TINTGIKIQEKDIPMLAETIMAADMLGVNPMYLANEGNVCMFVSPEVSEEVVRVLKNTKY
HHHCCCEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCHHHHHHHHHHCCCC
GKEAAVIGKVTQTKERQVLMEAKSGELKLIELLYGAELPRLC
CCHHHEEEHHHHHHHHHHHEECCCCCEEEEEEHHCCCCCCCC
>Mature Secondary Structure
MKLTKDLENIILDHGTGGLLSQDLISSIITAKLEDVHLGKMEDSAILEVSSRRLAMTTDS
CCCHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHEECCCCCCCCEEEECCCEEEEECCC
FVIDPIFFGEGNIGKVAVCGTVNDLAVSGAKPLYLSLALVLEEGFPIKDLEEILDSIRET
EEEEEEEECCCCCCCEEEECCCHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
AKEAGVYIVAGDTKVVKKGEVDKIFINTTGIGVFEGDVAPFSVNSIQEGDDIIITGQLGN
HHHCCEEEEECCCEEEECCCCCEEEEEECCCEEEECCCCCEECCCCCCCCCEEEEEECCC
HSIHILSIREGLGFEQRINSDCAPLNHMITELKNHFGDSIHCMRDITRGGLGTVLNEVSE
CEEEEEEECCCCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHH
TINTGIKIQEKDIPMLAETIMAADMLGVNPMYLANEGNVCMFVSPEVSEEVVRVLKNTKY
HHHCCCEEEECCCHHHHHHHHHHHHCCCCCEEEECCCCEEEEECCCHHHHHHHHHHCCCC
GKEAAVIGKVTQTKERQVLMEAKSGELKLIELLYGAELPRLC
CCHHHEEEHHHHHHHHHHHEECCCCCEEEEEEHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1526470 [H]