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The map label for this gene is colA [H]

Identifier: 30263213

GI number: 30263213

Start: 3035800

End: 3038682

Strand: Reverse

Name: colA [H]

Synonym: BA_3299

Alternate gene names: 30263213

Gene position: 3038682-3035800 (Counterclockwise)

Preceding gene: 30263214

Following gene: 30263212

Centisome position: 58.13

GC content: 33.26

Gene sequence:

>2883_bases
ATGAACAAGAACTTAAAATTTACTCAAATGATGATTGGGATTAGTACGATGGCATTATCATTTGGAAGTATTCAAACACA
CGTATCAGCAGAAGAAACAGCATCCTATAATATCTTACAGATGAAACCAATAGGGACAGAAACTTCAAAAGATGAAATTG
TACATGCTACAAAAGCGGACGAAACTTTGACTTTTGAAGAGCGTTTAAAAGTAGGAGATTTTTCACAACGTCCTACCCTG
GTTATGGAACGTGATGAAATTCAATTAAAGCAAAGCTACACTCTGGCAGAACTGAATAAAATGCCTAATAGCGAACTCAT
TGATACATTATCAAAAATTTCTTGGAATCAAATTACGGATTTATTTCAATTCAATCAAGATACGAAAGCATTTTATCAGA
ATAAAGAGCGCATGAACGTTATCATTAATGAATTAGGACAAAGAGGAAGGACGTTTACGAAAGAAAACTCAAAGGGCATT
GAAACATTTGTTGAAGTATTACGTTCTGCTTTTTATGTGGGATATTATAATAATGAATTAAGTTACTTAAAAGAGAGAAG
CTTCCATGAAAAATGTTTACCAGCATTGAAAGCGATTGCGAAAAATCCAAACTTTACATTAGGTACAGCTGAGCAAGATA
GAGTAGTAGCTGCGTATGGAAAATTAATTGGTAATGCCTCTAGTGATACTGAAACAGTACAGTATGCGGTAAATGTTTTA
AAACAATATAATGATAATCTTACTACGTATGTAAGCGATTATGCGAAAGGACAAGCTGTATATGAAATTGTAAAAGGAAT
TGATTATGATATACAGTCTTATTTGCAAGATACGAATAAGCAACCTAATGAAACAATGTGGTATGGAAAGATTGATAACT
TTATAAATGAGGTTAATAGAATTGCTCTCGTGGGGAATATAACAAATGAAAATAGTTGGCTAATTAATAATGGCATTTAT
TATGCAGGTCGTTTAGGGAAATTTCATAGTAATCCATACAAAGGATTGGAAGTTATTACACAAGCAATGAGCTTGTATCC
TCGTCTAAGTGGACCTTATTTTGTAGCAGTAGAACAAATTAAAACAAACTATGGTGGAAAAGATTATAGTGGAAAGGCAG
TAGATCTACAGAAAATACGTGAAGAAGGGAAACGACAATACTTACCTAAAACATATACATTTGATGACGGATCAATTGTC
TTCAAGACGGGAGATAAAGTAACAGAAGAAAAAATTAAGAGATTATATTGGGCAGCCAAAGAAGTAAAAGCGCAATATCA
CCGTGTAATTGGTAATGATAAAGCACTAGAACCGGGTAACGCTGATGATGTACTAACAATAGTAATTTATAATAATCCAG
ATGAATATCAATTAAATAGACAATTATATGGATATGAAACAAACAACGGTGGAATTTATATTGAAGAGAAGAGGACCTTC
TTTACATATGAGCGTACGCCAAAGCAGAGTATTTATAGTTTAGAAGAGTTATTCCGTCATGAATTCACTCATTATTTACA
AGGAAGGTATGAGGTTCCTGGTTTATTTGGAAGCGGAGAAATGTATCAAAATGAACGATTAACTTGGTTCCAAGAAGGGA
ATGCAGAATTTTTTGCAGGATCTACACGTACAAATAATGTTGTTCCACGTAAAAGTATGATAAGTGGCTTATCATCTGAT
CCAGCAAGCCGTTATACAGCAAAGCAAACTTTGTTCTCAAAATATGGATCATGGGACTTTTATAAGTATTCCTTTGCACT
ACAGTCATATTTGTATAATCATCAATTTGACACATTTGATAAGCTTCAAGATTTAATCCGTGTAAACGATGTGAAAAATT
ATGACTCATATCGTGAATCATTGAGCAACAATACACAATTGAATGCAGAATATCAAGCGTATATGCAGCAGTTGATTGAT
AATCAAGATAAATATAATGTACCGCAAGTAACAAATGATTATTTAATTCAACACGCACCAAAGCCGTTAGCTGAAGTGAA
AAACGAAATTGTGGATGTAGCAAATATAAAAGATGCAAAAATTACAAAATATGAGTCGCAATTCTTTAATACATTTACCG
TGGAAGGCAAGTACACAGGTGGTACATCAAAAGGTGAGTCTGAAGATTGGAAAACGATGAGTAAACAAGTAAATCGAACT
TTGGAGCAGTTATCCCAAAAAGGTTGGAGTGGTTATAAAACAGTTACAGCCTATTTTGTAAACTATCGTGTGAATGCAGC
TAACCAGTTTGAATATGATATTGTTTTTCATGGTGTTGCAACAGAGGAAAAGGAAAAAACAAATACTATAGTAAATATGA
ATGGACCATACAGCGGGATAGTAAATGAAGAGATTCAATTTCATAGCGATGGTACAAAAAGTGAAAATGGAAAAGTTACT
TCTTATCTATGGAACTTTGGAGATGGTACAACAAGTACAGAAGCAAATCCTACCCATGTATATGAAGAAAAAGGAACATA
CACTGTGGAACTAACTGTGAAAGATCGTAGAGGAAAAGAAAGCAAAGAACAAACAAAAGTTACTGTAAAACAAGATCCGC
AAACAGGTGAATTCCATGAAGAGGAGAAGGTACTCCTGTTTAATACGCTTGTAAAAGGAAATCTGGTTACTCCTGATCAA
ACAGATGTTTATACGTTTGATGTTACAGATACAAAAGAAGTAGATATTTCTGTGGTAAATGAACAAAATATTGGGATGAC
ATGGGTACTTTATCATGAATCAGACATGCAAAATTATGTAGCTTGTGGTGAAGATGAAGGAAATGTTATAAAGGGGAAAT
TCGAAGCGAAACCAGGCAAATATTATTTGAATGTTTATAAATTCGATGATAAAAATGGTGAATATTCATTATTGGTAAAA
TAA

Upstream 100 bases:

>100_bases
GGAAATTCATGTAATAAAATATAAAATGATACATATTATAATGATTTATTAAGATAAAGTGTTATATTTTTGGGTGGAAT
ATATAAGTGGAGGAAATGTA

Downstream 100 bases:

>100_bases
ATTCATTTCCTATATGTATACAAGGGTTGTATTTCAGGAGGAGTCTGTCACGATAGGCTGCTCCTATTTTTTCTCAAGTA
TTAGTTCTATAAATTGTTTA

Product: collagenase, putative

Products: NA

Alternate protein names: 120 kDa collagenase [H]

Number of amino acids: Translated: 960; Mature: 960

Protein sequence:

>960_residues
MNKNLKFTQMMIGISTMALSFGSIQTHVSAEETASYNILQMKPIGTETSKDEIVHATKADETLTFEERLKVGDFSQRPTL
VMERDEIQLKQSYTLAELNKMPNSELIDTLSKISWNQITDLFQFNQDTKAFYQNKERMNVIINELGQRGRTFTKENSKGI
ETFVEVLRSAFYVGYYNNELSYLKERSFHEKCLPALKAIAKNPNFTLGTAEQDRVVAAYGKLIGNASSDTETVQYAVNVL
KQYNDNLTTYVSDYAKGQAVYEIVKGIDYDIQSYLQDTNKQPNETMWYGKIDNFINEVNRIALVGNITNENSWLINNGIY
YAGRLGKFHSNPYKGLEVITQAMSLYPRLSGPYFVAVEQIKTNYGGKDYSGKAVDLQKIREEGKRQYLPKTYTFDDGSIV
FKTGDKVTEEKIKRLYWAAKEVKAQYHRVIGNDKALEPGNADDVLTIVIYNNPDEYQLNRQLYGYETNNGGIYIEEKRTF
FTYERTPKQSIYSLEELFRHEFTHYLQGRYEVPGLFGSGEMYQNERLTWFQEGNAEFFAGSTRTNNVVPRKSMISGLSSD
PASRYTAKQTLFSKYGSWDFYKYSFALQSYLYNHQFDTFDKLQDLIRVNDVKNYDSYRESLSNNTQLNAEYQAYMQQLID
NQDKYNVPQVTNDYLIQHAPKPLAEVKNEIVDVANIKDAKITKYESQFFNTFTVEGKYTGGTSKGESEDWKTMSKQVNRT
LEQLSQKGWSGYKTVTAYFVNYRVNAANQFEYDIVFHGVATEEKEKTNTIVNMNGPYSGIVNEEIQFHSDGTKSENGKVT
SYLWNFGDGTTSTEANPTHVYEEKGTYTVELTVKDRRGKESKEQTKVTVKQDPQTGEFHEEEKVLLFNTLVKGNLVTPDQ
TDVYTFDVTDTKEVDISVVNEQNIGMTWVLYHESDMQNYVACGEDEGNVIKGKFEAKPGKYYLNVYKFDDKNGEYSLLVK

Sequences:

>Translated_960_residues
MNKNLKFTQMMIGISTMALSFGSIQTHVSAEETASYNILQMKPIGTETSKDEIVHATKADETLTFEERLKVGDFSQRPTL
VMERDEIQLKQSYTLAELNKMPNSELIDTLSKISWNQITDLFQFNQDTKAFYQNKERMNVIINELGQRGRTFTKENSKGI
ETFVEVLRSAFYVGYYNNELSYLKERSFHEKCLPALKAIAKNPNFTLGTAEQDRVVAAYGKLIGNASSDTETVQYAVNVL
KQYNDNLTTYVSDYAKGQAVYEIVKGIDYDIQSYLQDTNKQPNETMWYGKIDNFINEVNRIALVGNITNENSWLINNGIY
YAGRLGKFHSNPYKGLEVITQAMSLYPRLSGPYFVAVEQIKTNYGGKDYSGKAVDLQKIREEGKRQYLPKTYTFDDGSIV
FKTGDKVTEEKIKRLYWAAKEVKAQYHRVIGNDKALEPGNADDVLTIVIYNNPDEYQLNRQLYGYETNNGGIYIEEKRTF
FTYERTPKQSIYSLEELFRHEFTHYLQGRYEVPGLFGSGEMYQNERLTWFQEGNAEFFAGSTRTNNVVPRKSMISGLSSD
PASRYTAKQTLFSKYGSWDFYKYSFALQSYLYNHQFDTFDKLQDLIRVNDVKNYDSYRESLSNNTQLNAEYQAYMQQLID
NQDKYNVPQVTNDYLIQHAPKPLAEVKNEIVDVANIKDAKITKYESQFFNTFTVEGKYTGGTSKGESEDWKTMSKQVNRT
LEQLSQKGWSGYKTVTAYFVNYRVNAANQFEYDIVFHGVATEEKEKTNTIVNMNGPYSGIVNEEIQFHSDGTKSENGKVT
SYLWNFGDGTTSTEANPTHVYEEKGTYTVELTVKDRRGKESKEQTKVTVKQDPQTGEFHEEEKVLLFNTLVKGNLVTPDQ
TDVYTFDVTDTKEVDISVVNEQNIGMTWVLYHESDMQNYVACGEDEGNVIKGKFEAKPGKYYLNVYKFDDKNGEYSLLVK
>Mature_960_residues
MNKNLKFTQMMIGISTMALSFGSIQTHVSAEETASYNILQMKPIGTETSKDEIVHATKADETLTFEERLKVGDFSQRPTL
VMERDEIQLKQSYTLAELNKMPNSELIDTLSKISWNQITDLFQFNQDTKAFYQNKERMNVIINELGQRGRTFTKENSKGI
ETFVEVLRSAFYVGYYNNELSYLKERSFHEKCLPALKAIAKNPNFTLGTAEQDRVVAAYGKLIGNASSDTETVQYAVNVL
KQYNDNLTTYVSDYAKGQAVYEIVKGIDYDIQSYLQDTNKQPNETMWYGKIDNFINEVNRIALVGNITNENSWLINNGIY
YAGRLGKFHSNPYKGLEVITQAMSLYPRLSGPYFVAVEQIKTNYGGKDYSGKAVDLQKIREEGKRQYLPKTYTFDDGSIV
FKTGDKVTEEKIKRLYWAAKEVKAQYHRVIGNDKALEPGNADDVLTIVIYNNPDEYQLNRQLYGYETNNGGIYIEEKRTF
FTYERTPKQSIYSLEELFRHEFTHYLQGRYEVPGLFGSGEMYQNERLTWFQEGNAEFFAGSTRTNNVVPRKSMISGLSSD
PASRYTAKQTLFSKYGSWDFYKYSFALQSYLYNHQFDTFDKLQDLIRVNDVKNYDSYRESLSNNTQLNAEYQAYMQQLID
NQDKYNVPQVTNDYLIQHAPKPLAEVKNEIVDVANIKDAKITKYESQFFNTFTVEGKYTGGTSKGESEDWKTMSKQVNRT
LEQLSQKGWSGYKTVTAYFVNYRVNAANQFEYDIVFHGVATEEKEKTNTIVNMNGPYSGIVNEEIQFHSDGTKSENGKVT
SYLWNFGDGTTSTEANPTHVYEEKGTYTVELTVKDRRGKESKEQTKVTVKQDPQTGEFHEEEKVLLFNTLVKGNLVTPDQ
TDVYTFDVTDTKEVDISVVNEQNIGMTWVLYHESDMQNYVACGEDEGNVIKGKFEAKPGKYYLNVYKFDDKNGEYSLLVK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PKD domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013510
- InterPro:   IPR007280
- InterPro:   IPR002169
- InterPro:   IPR013661
- InterPro:   IPR022409
- InterPro:   IPR000601 [H]

Pfam domain/function: PF01752 Peptidase_M9; PF08453 Peptidase_M9_N; PF00801 PKD; PF04151 PPC [H]

EC number: =3.4.24.3 [H]

Molecular weight: Translated: 110366; Mature: 110366

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS50093 PKD ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNLKFTQMMIGISTMALSFGSIQTHVSAEETASYNILQMKPIGTETSKDEIVHATKAD
CCCCCCHHHHHHHHHHHHHHHCCCEEECCHHHHCCEEEEEEECCCCCCCCCCEEEECCCC
ETLTFEERLKVGDFSQRPTLVMERDEIQLKQSYTLAELNKMPNSELIDTLSKISWNQITD
CEEEHHHHHCCCCCCCCCCEEEECCHHHHHHCCCHHHHHCCCCHHHHHHHHHCCHHHHHH
LFQFNQDTKAFYQNKERMNVIINELGQRGRTFTKENSKGIETFVEVLRSAFYVGYYNNEL
HHHCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCHH
SYLKERSFHEKCLPALKAIAKNPNFTLGTAEQDRVVAAYGKLIGNASSDTETVQYAVNVL
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
KQYNDNLTTYVSDYAKGQAVYEIVKGIDYDIQSYLQDTNKQPNETMWYGKIDNFINEVNR
HHHCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCE
IALVGNITNENSWLINNGIYYAGRLGKFHSNPYKGLEVITQAMSLYPRLSGPYFVAVEQI
EEEEEEECCCCCEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEH
KTNYGGKDYSGKAVDLQKIREEGKRQYLPKTYTFDDGSIVFKTGDKVTEEKIKRLYWAAK
HHCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHH
EVKAQYHRVIGNDKALEPGNADDVLTIVIYNNPDEYQLNRQLYGYETNNGGIYIEEKRTF
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEECCEEEEEEECCCCEEEEECCEE
FTYERTPKQSIYSLEELFRHEFTHYLQGRYEVPGLFGSGEMYQNERLTWFQEGNAEFFAG
EEECCCCHHHHHHHHHHHHHHHHHHHCCCEECCCEECCCCCCCCCCEEEEECCCCEEEEC
STRTNNVVPRKSMISGLSSDPASRYTAKQTLFSKYGSWDFYKYSFALQSYLYNHQFDTFD
CCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCHHH
KLQDLIRVNDVKNYDSYRESLSNNTQLNAEYQAYMQQLIDNQDKYNVPQVTNDYLIQHAP
HHHHHHHHHCCCCHHHHHHHHCCCCEECHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCC
KPLAEVKNEIVDVANIKDAKITKYESQFFNTFTVEGKYTGGTSKGESEDWKTMSKQVNRT
CCHHHHHHHHEECCCCCCCCEEHHHHHHCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
LEQLSQKGWSGYKTVTAYFVNYRVNAANQFEYDIVFHGVATEEKEKTNTIVNMNGPYSGI
HHHHHHCCCCCCCEEEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCCCC
VNEEIQFHSDGTKSENGKVTSYLWNFGDGTTSTEANPTHVYEEKGTYTVELTVKDRRGKE
CCCCEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCC
SKEQTKVTVKQDPQTGEFHEEEKVLLFNTLVKGNLVTPDQTDVYTFDVTDTKEVDISVVN
CHHHCEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEEC
EQNIGMTWVLYHESDMQNYVACGEDEGNVIKGKFEAKPGKYYLNVYKFDDKNGEYSLLVK
CCCCCEEEEEEECCCCCHHEECCCCCCCEEEEEEECCCCEEEEEEEEEECCCCCEEEEEC
>Mature Secondary Structure
MNKNLKFTQMMIGISTMALSFGSIQTHVSAEETASYNILQMKPIGTETSKDEIVHATKAD
CCCCCCHHHHHHHHHHHHHHHCCCEEECCHHHHCCEEEEEEECCCCCCCCCCEEEECCCC
ETLTFEERLKVGDFSQRPTLVMERDEIQLKQSYTLAELNKMPNSELIDTLSKISWNQITD
CEEEHHHHHCCCCCCCCCCEEEECCHHHHHHCCCHHHHHCCCCHHHHHHHHHCCHHHHHH
LFQFNQDTKAFYQNKERMNVIINELGQRGRTFTKENSKGIETFVEVLRSAFYVGYYNNEL
HHHCCCCHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHEEEEEECCHH
SYLKERSFHEKCLPALKAIAKNPNFTLGTAEQDRVVAAYGKLIGNASSDTETVQYAVNVL
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHH
KQYNDNLTTYVSDYAKGQAVYEIVKGIDYDIQSYLQDTNKQPNETMWYGKIDNFINEVNR
HHHCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHCE
IALVGNITNENSWLINNGIYYAGRLGKFHSNPYKGLEVITQAMSLYPRLSGPYFVAVEQI
EEEEEEECCCCCEEEECCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEH
KTNYGGKDYSGKAVDLQKIREEGKRQYLPKTYTFDDGSIVFKTGDKVTEEKIKRLYWAAK
HHCCCCCCCCCCEEHHHHHHHHHHHHCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHH
EVKAQYHRVIGNDKALEPGNADDVLTIVIYNNPDEYQLNRQLYGYETNNGGIYIEEKRTF
HHHHHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCEEECCEEEEEEECCCCEEEEECCEE
FTYERTPKQSIYSLEELFRHEFTHYLQGRYEVPGLFGSGEMYQNERLTWFQEGNAEFFAG
EEECCCCHHHHHHHHHHHHHHHHHHHCCCEECCCEECCCCCCCCCCEEEEECCCCEEEEC
STRTNNVVPRKSMISGLSSDPASRYTAKQTLFSKYGSWDFYKYSFALQSYLYNHQFDTFD
CCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHCCCCCHHH
KLQDLIRVNDVKNYDSYRESLSNNTQLNAEYQAYMQQLIDNQDKYNVPQVTNDYLIQHAP
HHHHHHHHHCCCCHHHHHHHHCCCCEECHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCC
KPLAEVKNEIVDVANIKDAKITKYESQFFNTFTVEGKYTGGTSKGESEDWKTMSKQVNRT
CCHHHHHHHHEECCCCCCCCEEHHHHHHCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHH
LEQLSQKGWSGYKTVTAYFVNYRVNAANQFEYDIVFHGVATEEKEKTNTIVNMNGPYSGI
HHHHHHCCCCCCCEEEEEEEEEEECCCCCEEEEEEEECCCCCCCCCCCEEEECCCCCCCC
VNEEIQFHSDGTKSENGKVTSYLWNFGDGTTSTEANPTHVYEEKGTYTVELTVKDRRGKE
CCCCEEECCCCCCCCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCEEEEEEEECCCCCC
SKEQTKVTVKQDPQTGEFHEEEKVLLFNTLVKGNLVTPDQTDVYTFDVTDTKEVDISVVN
CHHHCEEEEEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEEEEECCCCCCEEEEEEC
EQNIGMTWVLYHESDMQNYVACGEDEGNVIKGKFEAKPGKYYLNVYKFDDKNGEYSLLVK
CCCCCEEEEEEECCCCCHHEECCCCCCCEEEEEEECCCCEEEEEEEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8282691; 11792842 [H]