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The map label for this gene is yjjW

Identifier: 30065617

GI number: 30065617

Start: 4573489

End: 4574352

Strand: Reverse

Name: yjjW

Synonym: S4682

Alternate gene names: 30065617

Gene position: 4574352-4573489 (Counterclockwise)

Preceding gene: 30065618

Following gene: 30065609

Centisome position: 99.46

GC content: 54.51

Gene sequence:

>864_bases
ATGAACAGCAGATGCGCTTTAGTCAGTAAGATTATCCCCTTCTCCTGCGTTGACGGGCCAGGCAGTCGTCTGGCTCTGTT
TTTGCAGGGCTGCAATCTGCGCTGCAAAAACTGTCACAATCCGTGGACGATGGGACGTTGCAATGACTGTGGGGAGTGCG
TGCCACAGTGTCCGCATCAGGCGTTGCAGATTGTTGACGGCAAAGTGGTGTGGAACGCTGTGGTCTGCGAGCAATGTGAT
ACCTGCCTGAAGATGTGTCCGCAACATGCCACACCAATGGCGCAATCCATGAGCGTGGACGAAGTGCTTAGCCATGTGCG
CAAAGCGGTGCTGTTTATTGAAGGGATAACGGTAAGCGGCGGCGAAGCCACGACCCAACTGCCGTTTGTAGTGGCGCTGT
TTACTGCTATCAAAAACGATCCGCAACTGCGCCATCTCACCTGTCTGGTGGACAGTAACGGTATGTTGAGCGAAACCGGC
TGGGAAAAATTACTCCCGGTGTGTGACGGCGCAATGCTCGATCTCAAAGCATGGGGTAGCGAATGTCATCAACAATTAAC
CGGACGCGATAATCAACAGATTAAGCGCAGCATCTATTTGCTGGCAGAGCGCGGCAAGCTGGCGGAACTGCGTTTGCTGA
TGATCCCAGGCCAGGTGGATTATTTGCAACACATCGAAGAACTGGCGGCGTTTATCAAGGGACTTGGTGATGTTCCGGTT
CGCCTGAACGCGTTTCATGCCCACGGCGTGTATGGCGAGGCGCAAAGCTGGGCGAGCGCCACGCCGGAAGACGTTGAGCC
GTTGGCTGATGCATTAAAGGTGCGCGGGGTGAGCCGGTTGATATTTCCGGCGCTCTATTTGTGA

Upstream 100 bases:

>100_bases
AAATATCGCGCCGAAGGTTCACGCACCAACACCACCTGGCTGGGCGAAGAAGCCGCACGCAACACTCGTATTCTGGAACG
CCAGCCGCGCGTGATAAGCC

Downstream 100 bases:

>100_bases
CATGCCGGATACGGCCTACGGTTCGGCACAGACTTGTAGGCCTGATAAGACGCGTCAAGCGTCGCATCAGGCATTGTGCG
CCAACTGCCGGATGCGGCGT

Product: putative activating enzyme

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCD
TCLKMCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQVDYLQHIEELAAFIKGLGDVPV
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL

Sequences:

>Translated_287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCD
TCLKMCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQVDYLQHIEELAAFIKGLGDVPV
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL
>Mature_287_residues
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCD
TCLKMCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQVDYLQHIEELAAFIKGLGDVPV
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL

Specific function: Unknown

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1790839, Length=287, Percent_Identity=99.3031358885017, Blast_Score=589, Evalue=1e-170,
Organism=Escherichia coli, GI1787130, Length=287, Percent_Identity=26.8292682926829, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1790389, Length=254, Percent_Identity=23.2283464566929, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 31498; Mature: 31498

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01087 RADICAL_ACTIVATING ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
8.4 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
8.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQ
CCCCHHHHHHHCCCEECCCCCHHEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHCHHH
ALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSG
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEECC
GEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETGWEKLLPVCDGAMLDLKAWGS
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
ECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQVDYLQHIEELAAFIKGLGDVPV
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCE
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL
EEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTMGRCNDCGECVPQCPHQ
CCCCHHHHHHHCCCEECCCCCHHEEEEEECCCCEECCCCCCCCCCCCCCHHHHHHHCHHH
ALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEVLSHVRKAVLFIEGITVSG
HHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHHHHHHHHHHHHCCEEECC
GEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETGWEKLLPVCDGAMLDLKAWGS
CCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
ECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQVDYLQHIEELAAFIKGLGDVPV
HHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCE
RLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRGVSRLIFPALYL
EEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]