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The map label for this gene is prkB

Identifier: 30065359

GI number: 30065359

Start: 4318135

End: 4319004

Strand: Reverse

Name: prkB

Synonym: S4389

Alternate gene names: 30065359

Gene position: 4319004-4318135 (Counterclockwise)

Preceding gene: 30065360

Following gene: 30065357

Centisome position: 93.9

GC content: 52.87

Gene sequence:

>870_bases
ATGTCTGCCAAACATCCGGTCATTGCGGTAACAGGATCCAGCGGCGCGGGGACCACCACCACCAGCCTCGCGTTTCGTAA
AATATTCGCGCAGTTAAATCTGCATGCAGCTGAGGTGGAAGGCGACAGTTTTCACCGTTACACCCGCCCGGAAATGGACA
TGGCGATCCGCAAAGCGCGCGACGCCGGGCGGCATATCAGCTACTTCGGCCCCGAAGCGAACGACTTCGGCCTGCTGGAA
CAAACCTTCATTGAATACGGTCAGAGCGGCAAAGGGAAATCTCGCAAATATCTGCATACCTACGACGAAGCCGTACCCTG
GAATCAGGTACCGGGAACATTCACCCCCTGGCAACCTTTACCAGAACCCACTGATGTACTGTTTTATGAAGGTTTACACG
GCGGCGTAGTCACGCCACAGCATAACGTTGCGCAGCATGTGGACTTACTGGTCGGCGTAGTGCCTATCGTTAACCTTGAG
TGGATTCAAAAACTGATCCGCGACACCAGCGAGCGCGGGCACTCACGAGAAGCAGTGATGGACTCGGTAGTGCGTTCAAT
GGAAGACTATATCAACTACATCACACCGCAGTTTTCCCGCACCCATCTTAACTTCCAGCGCGTTCCCACCGTCGACACTT
CAAACCCGTTCGCGGCAAAAGGTATCCCGTCGCTGGATGAAAGCTTTGTGGTGATCCATTTTCGTAATCTGGAAGGGATC
GATTTCCCTTGGCTGCTGGCGATGTTGCAAGGCTCATTCATTTCCCACATCAATACGTTAGTGGTACCTGGCGGCAAAAT
GGGGCTGGCAATGGAATTAATTATGCTGCCGCTGGTGCAACGATTGATGGAAGGAAAGAAAATCGAGTAA

Upstream 100 bases:

>100_bases
TGCACGAAACGGTCAATATTATGCCGCGCAGTCAGTTTCGCGAATAACCTGCAACCGTCGGCGGACGAGATAATAGTGAT
AGCTAAGTCATGGAGTTACT

Downstream 100 bases:

>100_bases
CTTTGCTATTATGCCGGATAAAATACTATCCGGCTTCACAACGGGATAGTTAAGTCACGCGGCAACCACTTCATACGAGT
GAGTAATATTCACCGCTTTT

Product: phosphoribulokinase

Products: NA

Alternate protein names: PRK; PRKase; Phosphopentokinase

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSFHRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLE
QTFIEYGQSGKGKSRKYLHTYDEAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFAAKGIPSLDESFVVIHFRNLEGI
DFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIMLPLVQRLMEGKKIE

Sequences:

>Translated_289_residues
MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSFHRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLE
QTFIEYGQSGKGKSRKYLHTYDEAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFAAKGIPSLDESFVVIHFRNLEGI
DFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIMLPLVQRLMEGKKIE
>Mature_288_residues
SAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSFHRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQ
TFIEYGQSGKGKSRKYLHTYDEAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLEW
IQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFAAKGIPSLDESFVVIHFRNLEGID
FPWLLAMLQGSFISHINTLVVPGGKMGLAMELIMLPLVQRLMEGKKIE

Specific function: Unknown

COG id: COG3954

COG function: function code C; Phosphoribulokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoribulokinase family

Homologues:

Organism=Escherichia coli, GI2367214, Length=289, Percent_Identity=100, Blast_Score=599, Evalue=1e-173,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KPPR_ECOLI (P0AEX5)

Other databases:

- EMBL:   U18997
- EMBL:   U00096
- EMBL:   AP009048
- EMBL:   X61919
- PIR:   F65129
- RefSeq:   AP_004434.1
- RefSeq:   NP_417814.1
- ProteinModelPortal:   P0AEX5
- SMR:   P0AEX5
- MINT:   MINT-1286728
- STRING:   P0AEX5
- EnsemblBacteria:   EBESCT00000002765
- EnsemblBacteria:   EBESCT00000017999
- GeneID:   947862
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3318
- KEGG:   eco:b3355
- EchoBASE:   EB2268
- EcoGene:   EG12365
- eggNOG:   COG3954
- GeneTree:   EBGT00050000010763
- HOGENOM:   HBG294701
- OMA:   YIEGDAF
- ProtClustDB:   PRK15453
- BioCyc:   EcoCyc:EG12365-MONOMER
- Genevestigator:   P0AEX5
- InterPro:   IPR006082
- InterPro:   IPR006083
- PRINTS:   PR00478

Pfam domain/function: PF00485 PRK

EC number: =2.7.1.19

Molecular weight: Translated: 32344; Mature: 32213

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00567 PHOSPHORIBULOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSFHRYTRPEMDMAIRKAR
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHH
DAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYDEAVPWNQVPGTFTPWQPL
HCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCC
PEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLEWIQKLIRDTSERGHSREAVM
CCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHH
DSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFAAKGIPSLDESFVVIHFRNLEGI
HHHHHHHHHHHHHHCCCCHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
DFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIMLPLVQRLMEGKKIE
CHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSFHRYTRPEMDMAIRKAR
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHH
DAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYDEAVPWNQVPGTFTPWQPL
HCCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCC
PEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLEWIQKLIRDTSERGHSREAVM
CCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHH
DSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFAAKGIPSLDESFVVIHFRNLEGI
HHHHHHHHHHHHHHCCCCHHHCCCEEECCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCC
DFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIMLPLVQRLMEGKKIE
CHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 1756168; 7940673; 7984428; 7920643