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The map label for this gene is gntU

Identifier: 30065281

GI number: 30065281

Start: 4223019

End: 4224296

Strand: Direct

Name: gntU

Synonym: S4304

Alternate gene names: 30065281

Gene position: 4223019-4224296 (Clockwise)

Preceding gene: 161486383

Following gene: 30065283

Centisome position: 91.82

GC content: 55.87

Gene sequence:

>1278_bases
ATGAAGGCGCGTATGCACGCTTTCCTGGCTTTGATGGTGGTGTCCATGGGGGCTGGCCTTTTTTCCGGTATGCCGCTCGA
TAAAATCGCAGCGACGATGGAAAAAGGGATGGGAGGCACCCTCGGCTTCCTGGCGGTGGTTGTCGCCCTGGGAGCTATGT
TTGGCAAGATCTTATATGAAACCGGCGCAGTCGATCAGATTGCCGTCAAAATGCTCAAATCCTTCGGTCACAGCCGCGCG
CATTATGCCATCGGCCTTGCGGGGCTGGTCTGTGCGCTACCGCTGTTCTTTGAAGTGGCGATTGTTCTGCTGATTAGCGT
TGCTTTCTCAATGGCGCGCCACACCGGTACGAACCTGGTGAAGCTGGTAATCCCATTATTCGCAGGCGTGGCGGCCGCGG
CAGCGTTCCTGGTGCCTGGGCCAGCGCCAATGCTGCTGGCATCGCAGATGAATGCCGACTTTGGCTGGATGATCCTGATT
GGCCTGTGTGCGGCAATTCCGGGAATGATTATTGCCGGGCCGCTGTGGGGTAACTTCATCAGCCGTTACGTTGAGCTGCA
TATTCCTGACGACATCAGCGAACCGCATCTCGGCGAAGGTAAAATGCCATCTTTCGGATTCAGCCTGTCGCTGATCCTGT
TGCCGCTGGTGCTGGTGGGGCTGAAAACCATTGCCGCGCGTTTTGTGCCAGAAGGCTCTACCGCTTACGAATGGTTCGAG
TTTATTGGTCATCCGTTTACCGCGATTCTGGTTGCTTGTCTGGTAGCGATTTATGGCCTGGCAATGCGTCAGGGCATGCC
AAAAGACAAAGTGATGGAGATTTGCGGTCACGCGCTGCAACCGGCGGGGATCATTCTGCTGGTGATTGGTGCAGGCGGCG
TGTTCAAACAGGTGCTGGTTGACTCTGGCGTAGGTCCGGCACTGGGCGAAGCGTTAACCGGCATGGGCCTGCCGATTGCT
ATCACCTGCTTCGTGCTGGCTGCTGCAGTGCGCATCATTCAGGGGGCTGCCACCGTAGCCTGTTTAACGGCGGTGGGACT
GGTGATGCCAGTCATTGAACAACTGAACTACTCTGGTGCGCAAATGGCGGCACTGTCGATTTGTATCGCTGGTGGTTCGA
TTGTTGTCAGCCACGTTAACGACGCTGGTTTCTGGTTGTTCGGTAAATTTACCGGCGCGACCGAAGCCGAAACGCTGAAA
ACCTGGACCATGATGGAAACCATCCTCGGCACTGTTGGTGCCATCGTTGGGATGATTGCGTTCCAGCTGTTGAGTTAA

Upstream 100 bases:

>100_bases
AAGCACCATTGAGGTTATTAAAAAAGGCAAATAAGTAGTGACTACATTAACGCTTGTTTTAACAGCAGTAGGGTCTGTTT
TACTGCTGCTGTTTTTAGTC

Downstream 100 bases:

>100_bases
GTTTGTTCGCCCGGTAGTTGTGACGCTACCGGGCTCTTTTCGAAAAACTCTCCTCGTTACCCCTTCATCCACATTCGAAT
GCCGTCGAGGAACATCTGAG

Product: low affinity gluconate transporter

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: Gluconate permease; Gnt-I system [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRA
HYAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVPGPAPMLLASQMNADFGWMILI
GLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFE
FIGHPFTAILVACLVAIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALTGMGLPIA
ITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHVNDAGFWLFGKFTGATEAETLK
TWTMMETILGTVGAIVGMIAFQLLS

Sequences:

>Translated_425_residues
MKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRA
HYAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVPGPAPMLLASQMNADFGWMILI
GLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFE
FIGHPFTAILVACLVAIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALTGMGLPIA
ITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHVNDAGFWLFGKFTGATEAETLK
TWTMMETILGTVGAIVGMIAFQLLS
>Mature_425_residues
MKARMHAFLALMVVSMGAGLFSGMPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRA
HYAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVPGPAPMLLASQMNADFGWMILI
GLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHLGEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFE
FIGHPFTAILVACLVAIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALTGMGLPIA
ITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAGGSIVVSHVNDAGFWLFGKFTGATEAETLK
TWTMMETILGTVGAIVGMIAFQLLS

Specific function: Part of the gluconate utilization system Gnt-I; low- affinity intake of gluconate [H]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994939, Length=425, Percent_Identity=99.5294117647059, Blast_Score=835, Evalue=0.0,
Organism=Escherichia coli, GI1790716, Length=411, Percent_Identity=36.7396593673966, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI48994935, Length=416, Percent_Identity=35.5769230769231, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI2367370, Length=429, Percent_Identity=33.3333333333333, Blast_Score=236, Evalue=3e-63,
Organism=Escherichia coli, GI1788707, Length=420, Percent_Identity=35.4761904761905, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1789097, Length=432, Percent_Identity=31.4814814814815, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1790776, Length=425, Percent_Identity=30.5882352941176, Blast_Score=202, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 44227; Mature: 44227

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.6 %Met     (Translated Protein)
7.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCC
>Mature Secondary Structure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HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]