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The map label for this gene is nikC

Identifier: 30065245

GI number: 30065245

Start: 4186464

End: 4187297

Strand: Reverse

Name: nikC

Synonym: S4267

Alternate gene names: 30065245

Gene position: 4187297-4186464 (Counterclockwise)

Preceding gene: 30065246

Following gene: 30065244

Centisome position: 91.04

GC content: 60.31

Gene sequence:

>834_bases
GTGAACTTTTTCCTCTCTTCCCGCTGGTCGGTACGCCTGGCGCTGATCATTATCGCCCTGCTGGCGCTGATTGCGCTCAC
CAGCCAGTGGTGGCAGCCGTACGATCCACAGGCAATTGATTTGCCGTCGTGCCTGCTTTCGCCGGATGCGCAGCACTGGC
TGGGCACCGATCACTTAGGTCGCGATATTTTCTCGCGGCTGATGGCAGCGACCCGCGTGTCGCTCGGTTCGGTAATGGCC
TGCCTGCTGCTGGTGCTGACATTAGGGCTGGTTATTGGCGGCAGCGCCGGGTTGATTGGCGGGCACGTTGATCAGGCCAC
CATGCGCGTCGCCGATATGTTTATGACCTTCCCGACCTCGATTCTGTCGTTCTTTATGGTTGGCGTGCTCGGCACCGGGC
TGACCAACGTAATTATCGCCATCGCCCTGTCGCACTGGGCGTGGTATGCACGCATGGTGCGCAGCTTGGTGATTTCACTA
CGCCAACGCGAGTTTGTGCTGGCGTCACGGCTTTCCGGTGCGGGCCATGTGCGGGTGTTTGTCGATCATCTGGCAGGCGC
GGTGATCCCTTCGCTGCTGGTGCTGGCAACGCTGGATATCGGCCATATGATGCTGCACGTCGCGGGGATGTCTTTCCTTG
GCCTCGGTGTGACCGCGCCGACCGCCGAATGGGGCGTGATGATTAACGACGCGCGCCAGTATATCTGGACCCAGCCGCTG
CAAATGTTCTGGCCGGGGCTGGCGCTGTTTATCAGCGTGATGGCCTTTAACCTGGTGGGTGACGCACTGCGCGATCATCT
GGATCCTCATCTGGTGACGGAGCACGCACACTAA

Upstream 100 bases:

>100_bases
CGCTGATGATGGTGGTGGTTTTTGTGGTCTGTAATTTGATTGTCGATTTGCTCAACGCCGCGCTGGACCCGCGCATTCGT
CGTCATGAAGGAGCGCACGC

Downstream 100 bases:

>100_bases
TGCCGCAACAGATTGAACTACGTAATATCACGCTACAGGCCGCGCAGCCGCTGGTACACGGTGTATCGTTAACCCTGCAA
CGCGGGCGCGTGCTGGCGTT

Product: nickel transporter permease NikC

Products: Ni2; [Cytoplasm]; ADP; phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSCLLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMA
CLLLVLTLGLVIGGSAGLIGGHVDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVTAPTAEWGVMINDARQYIWTQPL
QMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHAH

Sequences:

>Translated_277_residues
MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSCLLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMA
CLLLVLTLGLVIGGSAGLIGGHVDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVTAPTAEWGVMINDARQYIWTQPL
QMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHAH
>Mature_277_residues
MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSCLLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMA
CLLLVLTLGLVIGGSAGLIGGHVDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVTAPTAEWGVMINDARQYIWTQPL
QMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHAH

Specific function: Involved in a nickel transport system, probably translocates nickel through the bacterial inner membrane [H]

COG id: COG1173

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789889, Length=277, Percent_Identity=98.9169675090253, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1787054, Length=259, Percent_Identity=38.2239382239382, Blast_Score=187, Evalue=6e-49,
Organism=Escherichia coli, GI1787760, Length=255, Percent_Identity=36.078431372549, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789964, Length=257, Percent_Identity=37.7431906614786, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1787498, Length=269, Percent_Identity=29.7397769516729, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1787549, Length=264, Percent_Identity=29.9242424242424, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788505, Length=227, Percent_Identity=23.3480176211454, Blast_Score=70, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR014157 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30305; Mature: 30305

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Ni2; [Periplasm]; ATP; H2O [C]

Specific reaction: Ni2+ [Periplasm] + ATP + H2O = Ni2+ [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]