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The map label for this gene is tnaB

Identifier: 30065019

GI number: 30065019

Start: 3912847

End: 3914094

Strand: Direct

Name: tnaB

Synonym: S4018

Alternate gene names: 30065019

Gene position: 3912847-3914094 (Clockwise)

Preceding gene: 30065018

Following gene: 30065020

Centisome position: 85.07

GC content: 46.63

Gene sequence:

>1248_bases
ATGACTGATCAAGCTGAAAAAAAGCACTCTGCATTTTGGGGTGTTATGGTTATAGCAGGTACAGTAATTGGTGGAGGTAT
GTTTGCTTTACCTGTTGATCTTGCCGGTGCCTGGTTTTTCTGGGGTGCCTTTATCCTTATCATTGCCTGGTTTTCAATGC
TTCATTCCGGGTTATTGTTATTAGAAGCAAATTTAAATTATCCTGTCGGCTCCAGTTTTAACACCATCACCAAAGATTTA
ATAGGTAACACCTGGAACATTATCAGCGGTATTACCGTTGCCTTCGTTCTCTATATCCTCACTTATGCCTATATCTCTGC
TAATGGTGCGATCATTAGTGAAACGATATCAATGAATTTGGGCTATCACGCTAATCCACGTATTGTCGGGATCTGCACAG
CCATTTTCGTTGCCAGCGTATTGTGGATAAGTTCGTTAGCCGCCAGTCGCATTACCTCATTGTTCCTCGGGCTGAAGATT
ATCTCCTTTGTGATCGTGTTTGGTTCTTTCTTCTTCCAGGTCGATTACTCCATCCTGCGCGATGCCACCAGCACCACTGC
GGGAACGTCTTACTTCCCGTATATCTTTATGGCTTTGCCGGTGTGTCTGGCGTCATTTGGTTTCCACGGCAATATTCCCA
GCCTGATTATTTGCTATGGCAAACGCAAAGATAAGTTAATCAAAAGCGTGGTATTTGGTTCGCTGCTGGCGCTGGTGATT
TATCTCTTCTGGCTCTATTGCACGATGGGGAATATTCCGCGCGAAAGCTTTAAGGCGATAATCTCCTCAGGCGGCAACAT
TGATTCGCTGGTGAAATCGTTCCTCGGCACCAAACAGCACGGCATTATCGAGTTTTGCCTGCTGGTGTTCTCTAACTTAG
CTGTTGCCAGTTCGTTCTTTGGTGTCACGCTGGGGTTGTTCGATTATCTGGCGGACCTGTTTAAGATTGATAACTCCCAC
GGCGGGCGTTTCAAAACCGTGCTGTTAACCTTCCTGCCACCTGCGTTGTTGTATCTGATCTTCCCGAACGGCTTTATTTA
CGGGATCGGCGGTGCCGGGCTGTGCGCCACCATCTGGGCGGTCATTATTCCCGCAGTGCTTGCAATCAAAGCTCGCAAGA
AGTTTCCCAATCAGATGTTCACGGTCTGGGGCGGCAATCTTATTCCGGCGATTGTCATTCTCTTTGGTATAACCGTGATT
TTGTGCTGGTTCGGCAACGTCTTTAACGTGTTACCTAAATTTGGCTAA

Upstream 100 bases:

>100_bases
AGAAGTTTAATTAATACTACAGAGTGGCTATAAGGATGTTAGCCACTCTCTTACCCTACATCCTCAATAACAAAAATAGC
CTTCCTCTAAAGGTGGCATC

Downstream 100 bases:

>100_bases
ATCCTTCAAGAAGCCAGCCATTCGCTGGCTTCTTGCCTCTCAGGAAATCACTTATGTCCAAATGGCAACTCGCCTTATCC
TCCTTCACCACGTATGCTTT

Product: tryptophan permease TnaB

Products: L-tryptophan [Cytoplasm]; Proton [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDL
IGNTWNIISGITVAFVLYILTYAYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKRKDKLIKSVVFGSLLALVI
YLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSH
GGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVI
LCWFGNVFNVLPKFG

Sequences:

>Translated_415_residues
MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDL
IGNTWNIISGITVAFVLYILTYAYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKRKDKLIKSVVFGSLLALVI
YLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSH
GGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVI
LCWFGNVFNVLPKFG
>Mature_414_residues
TDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLI
GNTWNIISGITVAFVLYILTYAYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKII
SFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKRKDKLIKSVVFGSLLALVIY
LFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHG
GRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVIL
CWFGNVFNVLPKFG

Specific function: Involved in tryptophan transport across the cytoplasmic membrane. Plays a role in transporting tryptophan which is to be used catabolically [H]

COG id: COG0814

COG function: function code E; Amino acid permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid/polyamine transporter 2 family. Mtr/TnaB/TyrP permease subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790145, Length=415, Percent_Identity=99.2771084337349, Blast_Score=818, Evalue=0.0,
Organism=Escherichia coli, GI1789552, Length=388, Percent_Identity=53.0927835051546, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1788218, Length=411, Percent_Identity=33.0900243309002, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002091
- InterPro:   IPR013061
- InterPro:   IPR013059
- InterPro:   IPR018227 [H]

Pfam domain/function: PF03222 Trp_Tyr_perm [H]

EC number: NA

Molecular weight: Translated: 45239; Mature: 45108

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00594 AROMATIC_AA_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLL
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTYAYISANGAIISETISMNL
EEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCC
GYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKIISFVIVFGSFFFQVDYSILR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKRKDKLIKSVVFGSLLALVI
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
YLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFF
HHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHH
VIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
TDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAFILIIAWFSMLHSGLLL
CCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTYAYISANGAIISETISMNL
EEECCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHCCC
GYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKIISFVIVFGSFFFQVDYSILR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIICYGKRKDKLIKSVVFGSLLALVI
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHH
YLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLGTKQHGIIEFCLLVFSNLAVASSFF
HHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPPALLYLIFPNGFIYGIGGAGLCATIWA
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHH
VIIPAVLAIKARKKFPNQMFTVWGGNLIPAIVILFGITVILCWFGNVFNVLPKFG
HHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-tryptophan [Periplasm]; Proton [Periplasm] [C]

Specific reaction: L-tryptophan [Periplasm] + Proton [Periplasm] = L-tryptophan [Cytoplasm] + Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]