Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 30065014

GI number: 30065014

Start: 3908664

End: 3910028

Strand: Direct

Name: trmE

Synonym: S4013

Alternate gene names: 30065014

Gene position: 3908664-3910028 (Clockwise)

Preceding gene: 30065013

Following gene: 30065017

Centisome position: 84.98

GC content: 56.63

Gene sequence:

>1365_bases
ATGAGCGATAATGACACTATCGTAGCCCAGGCCACGCCTCCGGGACGTGGCGGCGTTGGCATCCTGCGCATCTCCGGCTT
CAAAGCCCGTGAAGTTGCCGAAACCGTGCTGGGTAAACTGCCTAAGCCGCGCTACGCCGATTATCTTCCGTTTAAAGACG
CCGACGGCAGCGTGCTCGATCAGGGGATTGCGCTATGGTTCCCTGGCCCGAACTCGTTCACCGGCGAAGATGTGCTGGAA
CTGCAAGGTCATGGCGGTCCGGTGATCCTCGACCTGCTGTTAAAACGCATTCTGACCATTCCCGGCCTGCGGATTGCTCG
CCCTGGTGAGTTTTCCGAACGCGCGTTTCTTAACGATAAACTTGACTTAGCCCAGGCCGAGGCGATTGCCGATCTTATCG
ACGCCAGTTCGGAACAGGCGGCCCGTTCGGCACTTAACTCGCTGCAAGGCGCATTCTCCGCACGGGTTAATCATCTGGTA
GAAGCCCTCACCCACTTGCGCATTTACGTCGAAGCGGCAATTGATTTCCCTGATGAAGAGATCGATTTCCTCTCCGACGG
CAAAATTGAAGCCCAGCTTAATGACGTGATGGCCGATCTCGATGCAGTGCGTGCTGAAGCACGTCAGGGTAGTTTGTTGC
GCGAAGGGATGAAAGTAGTGATTGCCGGACGTCCTAATGCCGGTAAATCGAGCCTGTTAAACGCGCTGGCGGGCCGTGAA
GCGGCAATCGTAACCGATATCGCCGGAACCACGCGTGACGTGCTGCGTGAGCATATCCACATTGACGGAATGCCGCTGCA
TATCATCGACACTGCCGGGCTACGTGAGGCCAGTGACGAAGTGGAACGTATTGGTATCGAGCGCGCGTGGCAGGAAATTG
AACAGGCCGACCGCGTGCTGTTTATGGTCGATGGCACCACGACAGACGCCGTTGATCCGGCAGAGATCTGGCCGGAATTT
ATCGCCCGTCTGCCCGCGAAACTGCCGATCACCGTGGTACGCAATAAAGCCGATATCACCGGCGAAACGCTGGGAATGAG
TGAAGTGAACGGTCACGCGTTAATTCGTCTCTCGGCAAGGACTGGTGAAGGCGTGGACGTGCTGCGTAACCATCTCAAAC
AGAGCATGGGCTTTGACACCAATATGGAAGGCGGCTTCCTGGCGCGCCGTCGCCACTTACAGGCGCTGGAACAGGCAGCG
GAGCATTTGCAGCAAGGTAAAGCGCAACTGTTGGGAGCCTGGGCAGGTGAACTGCTGGCGGAAGAGTTGCGCCTGGCACA
GCAGAACTTAAGCGAAATCACCGGGGAATTTACTTCAGACGACCTGCTGGGGCGGATTTTCTCCAGCTTCTGTATTGGTA
AGTAA

Upstream 100 bases:

>100_bases
TGAGTTTTCGCTAAAATAAGGGCGGTCAGTTGACCGCCTTTTTTCTTTTCGTAGGGCGGATAAGCACCGCGCATCCGCCA
CACAAAGCAACAGGAACATC

Downstream 100 bases:

>100_bases
CCGCGCTTACGAAGCCGTATTCTGACTGTCAGATGCGGCTTCGCTTCATTGTTAGCACTCCTGTTATGCCTCAACCCTTT
TTTAAACATTAAAATTCTTA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 454; Mature: 453

Protein sequence:

>454_residues
MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPRYADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILTIPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEF
IARLPAKLPITVVRNKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALEQAA
EHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSFCIGK

Sequences:

>Translated_454_residues
MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPRYADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLE
LQGHGGPVILDLLLKRILTIPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEF
IARLPAKLPITVVRNKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALEQAA
EHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSFCIGK
>Mature_453_residues
SDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPRYADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLEL
QGHGGPVILDLLLKRILTIPGLRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVE
ALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGREA
AIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFI
ARLPAKLPITVVRNKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALEQAAE
HLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=467, Percent_Identity=36.4025695931477, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI193082988, Length=499, Percent_Identity=34.0681362725451, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI193082993, Length=461, Percent_Identity=35.5748373101952, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI2367268, Length=454, Percent_Identity=99.7797356828194, Blast_Score=907, Evalue=0.0,
Organism=Escherichia coli, GI87082120, Length=91, Percent_Identity=40.6593406593407, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17507259, Length=456, Percent_Identity=28.0701754385965, Blast_Score=168, Evalue=5e-42,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=30.4259634888438, Blast_Score=213, Evalue=7e-56,
Organism=Drosophila melanogaster, GI45550813, Length=485, Percent_Identity=32.9896907216495, Blast_Score=214, Evalue=8e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49250; Mature: 49118

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPRYADYLPFKDADGSVLD
CCCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPGLRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCH
LDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
IDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVRNKADITGETLGMSEVNGHALIRLSAR
EEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECC
TGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALEQAAEHLQQGKAQLLGAWAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQNLSEITGEFTSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPRYADYLPFKDADGSVLD
CCCCEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
QGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPGLRIARPGEFSERAFLNDK
CCEEEEECCCCCCCCCCHHEECCCCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHCCCH
LDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLVEALTHLRIYVEAAIDFPDEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCC
IDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMKVVIAGRPNAGKSSLLNALAGRE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHCCC
AAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLREASDEVERIGIERAWQEIEQADRVL
CEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
FMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVRNKADITGETLGMSEVNGHALIRLSAR
EEECCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEEECC
TGEGVDVLRNHLKQSMGFDTNMEGGFLARRRHLQALEQAAEHLQQGKAQLLGAWAGELLA
CCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EELRLAQQNLSEITGEFTSDDLLGRIFSSFCIGK
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA