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The map label for this gene is fadA

Identifier: 30064860

GI number: 30064860

Start: 3731589

End: 3732752

Strand: Direct

Name: fadA

Synonym: S3831

Alternate gene names: 30064860

Gene position: 3731589-3732752 (Clockwise)

Preceding gene: 30064859

Following gene: 30064863

Centisome position: 81.13

GC content: 58.25

Gene sequence:

>1164_bases
ATGGAACAGGTTGTCATTGTCGATGCAATTCGCACCCCGATGGGCCGTTCGAAGGGCGGTGCTTTTCGTAACGTGCGTGC
GGAAGATCTCTCCGCTCATTTAATGCGTAGCCTGCTGGCGCGTAACCCGGCGCTGGAAGCGGCGGCCCTCGACGATATTT
ACTGGGGTTGTGTGCAACAGACGCTGGAGCAGGGTTTTAACATCGCCCGTAACGCGGCGCTGCTGGCAGAAGTGCCGCAC
TCTGTCCCGGCGGTTACCGTCAATCGCTTGTGTGGTTCATCCATGCAGGCACTGCATGACGCAGCACGAATGATCATGAC
TGGCGATGCGCAGGCATGTCTGGTTGGCGGCGTGGAGCATATAGGCCATGTGCCGATGAGTCACGGCGTCGATTTTCACC
CCGGCCTGAGCCGCAATGTCGCCAAAGCGGCGGGCATGATGGGCTTAACGGCAGAAATGCTGGCGCGTATGCACGGTATC
AGCCGTGAAATGCAGGATGCCTTTGCCGCGCGGTCACACGCCCGCGCCTGGGCCGCCACGCAGTCGGCCGCATTTAAAAA
TGAAATCATCCCAACTGGTGGTCACGATGCCGACGGCGTCCTGAAGCAGTTTAATTACGACGAAGTGATTCGCCCGGAAA
CCACCGTGGAAGCCCTCGCCACGCTGCGTCCGGCGTTTGATCCAGTAAACGGTACGGTAACGGCGGGCACATCTTCTGCA
CTTTCCGATGGCGCAGCTGCCATGCTGGTGATGAGTGAAAGCCGCGCCCATGAATTAGGTCTTAAGCCGCGCGCTCGTGT
GCGTTCGATGGCGGTCGTTGGTTGTGACCCATCGATTATGGGTTACGGCCCGGTTCCGGCCTCGAAGCTGGCGCTGAAAA
AAGCGGGGCTTTCTGCCAGCGATATCGGCGTGTTTGAGATGAACGAAGCCTTTGCCGCGCAGATCCTGCCGTGCATTAAA
GATCTCGGGCTAATGGAGCAGATTGACGAGAAGATCAACCTCAATGGTGGTGCGATCGCGCTGGGTCATCCGCTGGGTTG
TTCCGGTGCGCGTATCAGCACCACGCTGCTGAATCTGATGGAACGCAAAGACGTTCAGTTTGGTCTGGCGACGATGTGTA
TCGGTCTGGGTCAGGGTATTGCGACGGTGTTTGAGCGGGTTTAA

Upstream 100 bases:

>100_bases
GGAAGGTCTGCGTAATAAAGCGCGTCATAACGAACCGTACTATCCTCCGGTTGAGCCAGCCCGTCCGGTTGGCGACCTGA
AAACGGCTTAAGGAGTCACA

Downstream 100 bases:

>100_bases
CCACTTGCCGGATGCGGCGTGAACGCCTTATCCGGTCTACCGATCCGGCACCAATGTAGGCCTGATAAGACGCGCCAGCG
TCGCATCAGGCATCGTGCAC

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 387; Mature: 387

Protein sequence:

>387_residues
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVNGTVTAGTSSA
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIK
DLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV

Sequences:

>Translated_387_residues
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVNGTVTAGTSSA
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIK
DLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV
>Mature_387_residues
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPH
SVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVNGTVTAGTSSA
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIK
DLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=396, Percent_Identity=42.9292929292929, Blast_Score=257, Evalue=2e-68,
Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=38.2278481012658, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI148539872, Length=403, Percent_Identity=38.4615384615385, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI4504327, Length=429, Percent_Identity=33.7995337995338, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI4557237, Length=402, Percent_Identity=34.3283582089552, Blast_Score=188, Evalue=9e-48,
Organism=Homo sapiens, GI194353979, Length=393, Percent_Identity=31.5521628498728, Blast_Score=147, Evalue=2e-35,
Organism=Escherichia coli, GI48994986, Length=387, Percent_Identity=99.2248062015504, Blast_Score=785, Evalue=0.0,
Organism=Escherichia coli, GI1787663, Length=405, Percent_Identity=45.1851851851852, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI1788554, Length=405, Percent_Identity=42.962962962963, Blast_Score=265, Evalue=4e-72,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=40.6483790523691, Blast_Score=260, Evalue=1e-70,
Organism=Escherichia coli, GI1788683, Length=418, Percent_Identity=34.9282296650718, Blast_Score=191, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI133906874, Length=401, Percent_Identity=41.1471321695761, Blast_Score=262, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=33.7209302325581, Blast_Score=190, Evalue=9e-49,
Organism=Caenorhabditis elegans, GI17535921, Length=403, Percent_Identity=33.4987593052109, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI25147385, Length=395, Percent_Identity=31.1392405063291, Blast_Score=158, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17535917, Length=388, Percent_Identity=27.0618556701031, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6322031, Length=391, Percent_Identity=39.386189258312, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6325229, Length=408, Percent_Identity=34.5588235294118, Blast_Score=186, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24655093, Length=402, Percent_Identity=40.0497512437811, Blast_Score=254, Evalue=9e-68,
Organism=Drosophila melanogaster, GI17648125, Length=402, Percent_Identity=39.3034825870647, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI17137578, Length=429, Percent_Identity=33.7995337995338, Blast_Score=198, Evalue=4e-51,
Organism=Drosophila melanogaster, GI24640423, Length=406, Percent_Identity=31.5270935960591, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012805
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 40847; Mature: 40847

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQ
CCCEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEH
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHCHHH
IGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAAT
HCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVNGTVTAGTSSA
HHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHH
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSAS
HCCCCEEEEEEECCHHHHCCCCCHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
DIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLM
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
ERKDVQFGLATMCIGLGQGIATVFERV
HHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPALEAAALDDIYWGCVQQ
CCCEEEEEHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAARMIMTGDAQACLVGGVEH
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHCHHH
IGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGISREMQDAFAARSHARAWAAT
HCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEALATLRPAFDPVNGTVTAGTSSA
HHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCHH
LSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCDPSIMGYGPVPASKLALKKAGLSAS
HCCCCEEEEEEECCHHHHCCCCCHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHCCCCCC
DIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINLNGGAIALGHPLGCSGARISTTLLNLM
CCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHH
ERKDVQFGLATMCIGLGQGIATVFERV
HHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA