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The map label for this gene is yihU

Identifier: 30064829

GI number: 30064829

Start: 3687003

End: 3687899

Strand: Direct

Name: yihU

Synonym: S3792

Alternate gene names: 30064829

Gene position: 3687003-3687899 (Clockwise)

Preceding gene: 30064822

Following gene: 30064830

Centisome position: 80.16

GC content: 55.3

Gene sequence:

>897_bases
ATGGCAGCAATCGCGTTTATCGGTTTAGGACAAATGGGTTCGCCAATGGCGAGCAATTTATTGCAGCAAGGGCATCAACT
TCGCGTCTTTGATGTGAACGCCGAAGCTGTGCGGCATCTGGTAGACAAAGGTGCGACTCCCGCCGCCAACCCGGCGCAGG
CCGCTAAAGATGCCGAATTTATCATTACCATGTTGCCGAATGGCGATCTGGTGCGCAACGTGTTGTTCGGTGAAAACGGC
GTTTGCGAAGGCTTATCTACCGATGCGCTGGTCATTGATATGTCCACCATCCATCCGCTGCAAACCGATAAATTGATTGC
CGATATGCAAGCCAAAGGCTTCAGCATGATGGATGTTCCGGTAGGCCGTACTTCTGCAAATGCCATTACCGGTACTCTGT
TACTGCTGGCTGGCGGCACCGCTGAACAAGTTGAACGTGCCACACCGATCCTGATGGCGATGGGCAGTGAGTTGATCAAC
GCTGGCGGTCCGGGCATGGGGATCCGCGTTAAGCTCATCAACAACTACATGAGCATCGCGCTCAATGCGCTTTCGGCAGA
AGCCGCCGTTTTGTGCGAAGCCCTGAATCTTCCCTTCGATGTTGCCGTCAAAGTGATGAGCGGTACCGCCGCCGGTAAAG
GTCACTTCACCACTTCCTGGCCAAACAAAGTCCTCAGCGGAGATCTTTCTCCCGCCTTCATGATCGATCTTGCCCATAAG
GATCTTGGCATCGCCCTTGATGTCGCCAACCAGCTGCATGTGCCAATGCCGCTGGGGGCCGCCTCACGGGAGGTTTATAG
CCAGGCGCGCGCAGCGGGTCGCGGTCGCCAGGACTGGTCCGCCATTCTGGAACAGGTCCGTGTCAGTGCCGGGATGACTG
CCAAAGTAAAAATGTAA

Upstream 100 bases:

>100_bases
GTCAATTTTTTGACGATAAATTACAAATTCGATCAAAAACAGACAAGCAAAGCATGTCATAAATGACAAAAAGTGACATC
ACTGTATTCAGGAGAGGGTT

Downstream 100 bases:

>100_bases
CGACTGGATAAAGGAATAAATGAATGAATAAGTACACCATCAACGACATTACGCGCGCATCGGGCGGTTTTGCCATGCTG
GCGGTCGATCAGCGCGAAGC

Product: putative dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKGATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENG
VCEGLSTDALVIDMSTIHPLQTDKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHK
DLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM

Sequences:

>Translated_298_residues
MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKGATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENG
VCEGLSTDALVIDMSTIHPLQTDKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHK
DLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM
>Mature_297_residues
AAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKGATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGV
CEGLSTDALVIDMSTIHPLQTDKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELINA
GGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHKD
LGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM

Specific function: Unknown

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI23308751, Length=291, Percent_Identity=31.6151202749141, Blast_Score=136, Evalue=2e-32,
Organism=Homo sapiens, GI40556376, Length=280, Percent_Identity=25.7142857142857, Blast_Score=105, Evalue=4e-23,
Organism=Escherichia coli, GI1790315, Length=298, Percent_Identity=100, Blast_Score=603, Evalue=1e-174,
Organism=Escherichia coli, GI145693186, Length=280, Percent_Identity=36.7857142857143, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1786719, Length=289, Percent_Identity=31.1418685121107, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789092, Length=292, Percent_Identity=29.7945205479452, Blast_Score=115, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI17557316, Length=283, Percent_Identity=29.6819787985866, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24655230, Length=292, Percent_Identity=33.9041095890411, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI19922568, Length=292, Percent_Identity=33.9041095890411, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI28574115, Length=287, Percent_Identity=22.9965156794425, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24655240, Length=64, Percent_Identity=53.125, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): YIHU_ECOLI (P0A9V8)

Other databases:

- EMBL:   L19201
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   S40826
- RefSeq:   AP_003926.1
- RefSeq:   NP_418318.1
- ProteinModelPortal:   P0A9V8
- SMR:   P0A9V8
- DIP:   DIP-48142N
- MINT:   MINT-1289833
- STRING:   P0A9V8
- EnsemblBacteria:   EBESCT00000000519
- EnsemblBacteria:   EBESCT00000015016
- GeneID:   948372
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW3853
- KEGG:   eco:b3882
- EchoBASE:   EB1793
- EcoGene:   EG11847
- eggNOG:   COG2084
- GeneTree:   EBGT00050000009837
- HOGENOM:   HBG729179
- OMA:   RGFSMMD
- ProtClustDB:   PRK15461
- Genevestigator:   P0A9V8
- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR22981

Pfam domain/function: PF03446 NAD_binding_2; SSF48179 6DGDH_C_like

EC number: 1.1.-.- [C]

Molecular weight: Translated: 31159; Mature: 31027

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: ACT_SITE 171-171 BINDING 96-96 BINDING 240-240

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKGATPAANPAQAAKDAEF
CCEEEEEECCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHCCCCEE
IITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQTDKLIADMQAKGFSMMDVP
EEEECCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEECC
VGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELINAGGPGMGIRVKLINNYMSIA
CCCCCCHHHHEEEEEEECCCHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHH
LNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHK
HHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCCCCEECCCCCHHHCCCCCCEEEEEECCC
DLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEC
>Mature Secondary Structure 
AAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKGATPAANPAQAAKDAEF
CEEEEEECCCCCCHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCCCCCHHHHCCCCEE
IITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQTDKLIADMQAKGFSMMDVP
EEEECCCCHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEECC
VGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELINAGGPGMGIRVKLINNYMSIA
CCCCCCHHHHEEEEEEECCCHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEEEHHHHHHHH
LNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTTSWPNKVLSGDLSPAFMIDLAHK
HHHHHHHHHHHHHHHCCCHHHHEEEECCCCCCCCCEECCCCCHHHCCCCCCEEEEEECCC
DLGIALDVANQLHVPMPLGAASREVYSQARAAGRGRQDWSAILEQVRVSAGMTAKVKM
CCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8346018; 9278503