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The map label for this gene is yjbN

Identifier: 30064638

GI number: 30064638

Start: 3443535

End: 3444554

Strand: Direct

Name: yjbN

Synonym: S3574

Alternate gene names: 30064638

Gene position: 3443535-3444554 (Clockwise)

Preceding gene: 30064637

Following gene: 161486419

Centisome position: 74.87

GC content: 53.24

Gene sequence:

>1020_bases
ATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGTGTTGCACCAAT
GCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTTTATACCGAAATGGTGA
CCACAGGGGCGATAATTCACGGTAAAGGTGATTATCTGGCGTACAGTGAAGAAGAACATCCGGTAGCGTTGCAACTGGGC
GGTAGCGATCCGGCGGCGCTGGCGCAGTGTGCAAAGCTGGCAGAAGCGCGCGGATATGATGAGATCAACCTGAATGTCGG
CTGCCCGTCTGACCGGGTGCAGAACGGCATGTTTGGTGCGTGTCTGATGGGTAATGCGCAGCTGGTTGCCGACTGCGTGA
AAGCGATGCGCGATGTGGTGTCGATTCCGGTGACGGTGAAAACGCGTATTGGCATCGACGACCAGGACAGCTATGAATTT
CTCTGCGATTTCATCAATACCGTTTCCGGCAAAGGCGAGTGTGAGATGTTCATCATCCACGCACGTAAAGCCTGGCTTTC
GGGGTTAAGCCCGAAAGAAAACCGTGAAATCCCGCCGCTCGATTATCCGCGTGTGTATCAACTGAAGCGTGACTTCCCGC
ATCTGACAATGTCGATTAACGGTGGTATCAAGTCGCTGGAAGAAGCTAAAGCGCATTTGCAACATATGGATGGCGTGATG
GTCGGGCGCGAGGCGTATCAGAATCCGGGTATTCTGGCGGCGGTAGACCGGGAGATCTTTGGTTCCTCGGATACCGATGC
CGATCCGGTGGCAGTAGTGCGTGCCATGTATCCGTACATTGAGCATGAACTCAGCCAGGGGACGTATCTCGGCCATATTA
TCCGGCATATGCTGGGTTTGTTCCAGGGTATTCCTGGCGCGCGGCAGTGGCGGCGTTATTTAAGTGAAAATGCCCATAAA
GCGGGTGCTGACATTAACGTGCTGGAACACGCGCTCAAACTGGTGGCGGATAAGCGTTAA

Upstream 100 bases:

>100_bases
CCCCGCTATTGAGCCTCCTGAACAGTGAAGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGA
AAATGCACGGTAATTCTGAA

Downstream 100 bases:

>100_bases
CTTTTCACCAAAAAGTAGTCAAATTCACCACGCCCTGCGCACCGTCGCGGGGCGTTTTGCTGTTAAATCAATAGATTATT
TTTGGCATGATTCTTGTAAT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLG
GSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEF
LCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQHMDGVM
VGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHK
AGADINVLEHALKLVADKR

Sequences:

>Translated_339_residues
MQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLG
GSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEF
LCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQHMDGVM
VGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHK
AGADINVLEHALKLVADKR
>Mature_339_residues
MQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLG
GSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEF
LCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQHMDGVM
VGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHK
AGADINVLEHALKLVADKR

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI31742496, Length=308, Percent_Identity=24.025974025974, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI145693211, Length=330, Percent_Identity=99.3939393939394, Blast_Score=687, Evalue=0.0,
Organism=Escherichia coli, GI1788462, Length=251, Percent_Identity=25.8964143426295, Blast_Score=63, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323560, Length=245, Percent_Identity=26.9387755102041, Blast_Score=71, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 37806; Mature: 37806

Theoretical pI: Translated: 6.57; Mature: 6.57

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIH
CCCCCCCCCCCCCCCCEECCCEEECCCCCCCHHHHHHHHHHHHCCCEEEEHHHHHCEEEE
GKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGA
CCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEE
CLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGECEMFIIH
EEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEE
ARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQHMDGVM
EHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCHH
VGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEHELSQGTYLGHIIRHMLGL
CCHHHHCCCCEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR
HHCCCCHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYFLRLLSRNTLLYTEMVTTGAIIH
CCCCCCCCCCCCCCCCEECCCEEECCCCCCCHHHHHHHHHHHHCCCEEEEHHHHHCEEEE
GKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEARGYDEINLNVGCPSDRVQNGMFGA
CCCCEEEECCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHCCCCEEE
CLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDDQDSYEFLCDFINTVSGKGECEMFIIH
EEECCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEEE
ARKAWLSGLSPKENREIPPLDYPRVYQLKRDFPHLTMSINGGIKSLEEAKAHLQHMDGVM
EHHHHHCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCHH
VGREAYQNPGILAAVDREIFGSSDTDADPVAVVRAMYPYIEHELSQGTYLGHIIRHMLGL
CCHHHHCCCCEEEEECHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
FQGIPGARQWRRYLSENAHKAGADINVLEHALKLVADKR
HHCCCCHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]