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The map label for this gene is glnE

Identifier: 30064401

GI number: 30064401

Start: 3174589

End: 3177429

Strand: Reverse

Name: glnE

Synonym: S3299

Alternate gene names: 30064401

Gene position: 3177429-3174589 (Counterclockwise)

Preceding gene: 30064402

Following gene: 30064400

Centisome position: 69.08

GC content: 55.3

Gene sequence:

>2841_bases
ATGAAGCCGCTCTCTTCACCGTTACAGCAGTACTGGCAGACCGTTGTTGAGCGGCTGCCAGAGCTTTTAGCCGAGGAATC
ACTTAGTGCACAGGCGAAGTCAGTACTTACTTTCAGTGATTTTGTGCAGGACAGCGTGATTGCGCATCCAGAGTGGCTGA
CGGAACTGGAAAGCCAACCGCCACAGGCTGACGAATGGCAACATTACAGCGTGTGGTTGCAGGAGGCGCTCAGTAATGTG
AGCGACGAAGCCGGGTTAATGCGCGAGCTGCGGCTATTCCGGCGGCGCATTATGGTGCGCATCGCATGGGCGCAAACGCT
GGCACTGGTTACTGAAGAGAGCATATTGCAGCAGCTCAGTTATCTGGCGGAGACGCTGATTGTTGCGGCGCGTGACTGGC
TGTATGACGCCTGCTGCCGCGAGTGGGGAACGCCGTGCAATGCGCAGGGCGAAGCGCAACCGCTGCTGATCTTAGGCATG
GGTAAGCTGGGCGGCGGGGAACTGAATTTCTCCTCTGATATCGATCTGATTTTTGCCTGGCCGGAACATGGTTGTACGCA
GGGCGGACGCCGGGAACTGGATAACGCGCAGTTTTTTACCCGCATGGGGCAGCGGCTGATTAAAGTGCTGGATCAACCAA
CGCAGGATGGCTTCGTCTATCGCGTGGATATGCGACTGCGTCCGTTTGGCGAAAGTGGCCCGCTGGTGCTGAGCTTTGCC
GCGTTGGAGGATTATTACCAGGAGCAGGGACGCGACTGGGAGCGTTACGCGATGGTCAAAGCGCGGATTATGGGCGATAG
CGAAGGCGTCTATGCTAACGAGTTGCGTGCGATGCTGCGCCCGTTTGTTTTCCGTCGTTACATCGATTTCAGCGTGATTC
AGTCGCTGCGCAACATGAAAGGGATGATTGCCCGTGAAGTGCGTCGACGTGGTTTGACCGACAATATCAAACTCGGCGCA
GGCGGCATTCGCGAAATTGAATTTATCGTTCAGGTGTTCCAGCTCATTCGCGGTGGGCGCGAACCGTTGCTGCAGTCGCG
CTCTTTACTGCCAACGCTCAGCGCCATTGCCGCGCTGCATCTGCTTTCTGAAAACGATGCTGAACAATTGCGAGTGGCAT
ATCTGTTCCTGCGGCGTCTGGAAAACCTGCTGCAAAGCATTAACGACGAACAAACCCAAACGCTTCCTTCTGATGAGCTT
AACCGTGCGCGGCTGGCGTGGGCGATGGACTTTGCTGACTGGCCGCAACTGACCGGGGCGCTGACCGCACATATGACCAA
TGTGCGCCGGGTGTTTAATGAATTGATTGGCGACGACGAAAGTGAAACTCAGGAAGAGTCGCTGTCGGAACAGTGGCGTG
AGCTGTGGCAGGATGCGTTGCAGGAAGATGACACTACGCCAGTGCTGGCGCATCTTAGCGAGGATGATCGCAAACAGGTC
CTAACGATGATTGCTGATTTCCGCAAAGAGCTGGATAAGCGTACTATCGGGCCGCGAGGACGTCAGGTGCTCGACCATCT
GATGCCACATCTGCTAAGTGATGTCTGTGCGCGTGAAGACGCTGCTGTCACGCTGTCGCGCATTACCGCCTTGCTGGTGG
GGATTGTTACCCGCACAACCTATTTAGAATTGCTCAGTGAATTCCCTGCGGCGCTTAAACATTTGATTTCTCTGTGTGCC
GCATCGCCGATGATTGCCAGCCAGCTAGCGCGTTATCCATTATTGCTGGATGAATTGCTCGATCCAAATACCCTTTATCA
GCCGACGGCGACCGATGCCTACCGTGATGAACTGCGCCAGTATTTGCTGCGCGTGCCGGAAGATGACGAAGAACAACAGC
TTGAGGCGCTGCGTCAGTTCAAACAGGCGCAGCTGTTACGCATTGCGGCAGCGGATATCGCCGGTACGCTACCGGTAATG
AAAGTGAGCGATCACTTAACCTGGCTGGCAGAAGCTATGATAGATGCCGTCGTTCAGCAGGCGTGGGGTCAAATGGTGGC
CCGCTACGGTAAGCCAAATCACCTCAACGAACGTGAAGGGCGCGGTTTTGCGGTGGTCGGCTACGGCAAGCTGGGCGGCT
GGGAGTTAGGCTACAGTTCCGATCTTGACCTTATCTTCCTCCATGATTGCCCAATGGATGCGATGACTGACGGCGAGCGG
GAAATCGACGGGCGGCAGTTTTATCTGCGTCTGGCGCAACGCATTATGCATCTGTTCAGTACGCGTACCTCTTCCGGCAT
TTTGTATGAAGTGGATGCTCGACTGCGTCCGTCCGGGGCGGCGGGAATGCTGGTGACATCCGCAGAAGCATTTGCCGATT
ATCAGAAAAACGAGGCCTGGACGTGGGAACATCAGGCGCTGGTGCGTGCGCGTGTAGTGTACGGCGATCCGCAGCTCACT
GCGCACTTTGACGCGGTGCGTCGTGAGATTATGACACTGCCGCGAGAAGGTAAAACTCTGCAAACGGAAGTGCGGGAAAT
GCGCGAGAAAATGCGCGCTCATCTCGGCAATAAACATCGTAATCGCTTTGATATCAAAGCCGATGAAGGCGGAATTACCG
ATATCGAATTTATTACCCAATATCTGGTGTTGCGCTACGCTCATGAAAAACCGAAGTTAACGCGCTGGTCAGACAACGTG
CGTATTCTGGAGCTGCTGGCGCAAAACGACATTATGGAAGAGCAGGAAGCGATGGCGCTGACCCGTGCTTACACGACGCT
GCGCGATGAACTTCATCATCTGGCATTACAGGAATTGCCGGGCCATGTGTCGGAGGATTGCTTCACCGCAGAGCGTGATC
TGGTGCGGGCAAGCTGGCAGAAGTGGCTGGTGGAAGAATGA

Upstream 100 bases:

>100_bases
CAACGCATTGAAATTGAACATTTCCGTAATGAGGCAAACAATCAGGAACCGTTCTGGTTGCACAGCGGAAAACGTTAATC
AGGCAAGGATAAAATCGCTT

Downstream 100 bases:

>100_bases
AGTATGGTATTATCGCGCGCAAATTTTGAATCTCTCAGGAGACAGGAATGAAAGTAACGCTGCCAGAGTTTGAACGTGCA
GGAGTGATGGTGGTTGGTGA

Product: bifunctional glutamine-synthetase adenylyltransferase/deadenyltransferase

Products: NA

Alternate protein names: Glutamine-synthetase adenylyltransferase; ATase; [Glutamate--ammonia-ligase] adenylyltransferase [H]

Number of amino acids: Translated: 946; Mature: 946

Protein sequence:

>946_residues
MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQDSVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNV
SDEAGLMRELRLFRRRIMVRIAWAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGFVYRVDMRLRPFGESGPLVLSFA
ALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELRAMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGA
GGIREIEFIVQVFQLIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQTQTLPSDEL
NRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSEQWRELWQDALQEDDTTPVLAHLSEDDRKQV
LTMIADFRKELDKRTIGPRGRQVLDHLMPHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCA
ASPMIASQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQLLRIAAADIAGTLPVM
KVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGER
EIDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQLT
AHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIEFITQYLVLRYAHEKPKLTRWSDNV
RILELLAQNDIMEEQEAMALTRAYTTLRDELHHLALQELPGHVSEDCFTAERDLVRASWQKWLVEE

Sequences:

>Translated_946_residues
MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQDSVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNV
SDEAGLMRELRLFRRRIMVRIAWAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGFVYRVDMRLRPFGESGPLVLSFA
ALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELRAMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGA
GGIREIEFIVQVFQLIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQTQTLPSDEL
NRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSEQWRELWQDALQEDDTTPVLAHLSEDDRKQV
LTMIADFRKELDKRTIGPRGRQVLDHLMPHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCA
ASPMIASQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQLLRIAAADIAGTLPVM
KVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGER
EIDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQLT
AHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIEFITQYLVLRYAHEKPKLTRWSDNV
RILELLAQNDIMEEQEAMALTRAYTTLRDELHHLALQELPGHVSEDCFTAERDLVRASWQKWLVEE
>Mature_946_residues
MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQDSVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNV
SDEAGLMRELRLFRRRIMVRIAWAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGFVYRVDMRLRPFGESGPLVLSFA
ALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELRAMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGA
GGIREIEFIVQVFQLIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQTQTLPSDEL
NRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSEQWRELWQDALQEDDTTPVLAHLSEDDRKQV
LTMIADFRKELDKRTIGPRGRQVLDHLMPHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCA
ASPMIASQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQLLRIAAADIAGTLPVM
KVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGER
EIDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQLT
AHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIEFITQYLVLRYAHEKPKLTRWSDNV
RILELLAQNDIMEEQEAMALTRAYTTLRDELHHLALQELPGHVSEDCFTAERDLVRASWQKWLVEE

Specific function: Adenylation and deadenylation of glutamate--ammonia ligase [H]

COG id: COG1391

COG function: function code OT; Glutamine synthetase adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glnE family [H]

Homologues:

Organism=Escherichia coli, GI1789433, Length=946, Percent_Identity=98.9429175475687, Blast_Score=1912, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023057
- InterPro:   IPR005190
- InterPro:   IPR013546 [H]

Pfam domain/function: PF08335 GlnD_UR_UTase; PF03710 GlnE [H]

EC number: =2.7.7.42 [H]

Molecular weight: Translated: 108393; Mature: 108393

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQDSVIAHPEWLTELESQP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
PQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIAWAQTLALVTEESILQQLS
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGMGKLGGGELNFSSDIDLIFAW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEC
PEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGFVYRVDMRLRPFGESGPLVLSFA
CCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCEEEEHH
ALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELRAMLRPFVFRRYIDFSVIQSLRNMK
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQLIRGGREPLLQSRSLLPTLSAIAALH
HHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LLSENDAEQLRVAYLFLRRLENLLQSINDEQTQTLPSDELNRARLAWAMDFADWPQLTGA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHH
LTAHMTNVRRVFNELIGDDESETQEESLSEQWRELWQDALQEDDTTPVLAHLSEDDRKQV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHH
LTMIADFRKELDKRTIGPRGRQVLDHLMPHLLSDVCAREDAAVTLSRITALLVGIVTRTT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YLELLSEFPAALKHLISLCAASPMIASQLARYPLLLDELLDPNTLYQPTATDAYRDELRQ
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
YLLRVPEDDEEQQLEALRQFKQAQLLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AWGQMVARYGKPNHLNEREGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGER
HHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHCCCCH
EIDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAW
HCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHEECHHHHHHHHCCCCC
TWEHQALVRARVVYGDPQLTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHR
CHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
NRFDIKADEGGITDIEFITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMAL
CCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TRAYTTLRDELHHLALQELPGHVSEDCFTAERDLVRASWQKWLVEE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQDSVIAHPEWLTELESQP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
PQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIAWAQTLALVTEESILQQLS
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGMGKLGGGELNFSSDIDLIFAW
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEC
PEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGFVYRVDMRLRPFGESGPLVLSFA
CCCCCCCCCHHCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCCEEEEHH
ALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELRAMLRPFVFRRYIDFSVIQSLRNMK
HHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQLIRGGREPLLQSRSLLPTLSAIAALH
HHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LLSENDAEQLRVAYLFLRRLENLLQSINDEQTQTLPSDELNRARLAWAMDFADWPQLTGA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHH
LTAHMTNVRRVFNELIGDDESETQEESLSEQWRELWQDALQEDDTTPVLAHLSEDDRKQV
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCHHHHHH
LTMIADFRKELDKRTIGPRGRQVLDHLMPHLLSDVCAREDAAVTLSRITALLVGIVTRTT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YLELLSEFPAALKHLISLCAASPMIASQLARYPLLLDELLDPNTLYQPTATDAYRDELRQ
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHH
YLLRVPEDDEEQQLEALRQFKQAQLLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQ
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AWGQMVARYGKPNHLNEREGRGFAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGER
HHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCHHHCCCCH
EIDGRQFYLRLAQRIMHLFSTRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAW
HCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHEECHHHHHHHHCCCCC
TWEHQALVRARVVYGDPQLTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHR
CHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCC
NRFDIKADEGGITDIEFITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMAL
CCEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TRAYTTLRDELHHLALQELPGHVSEDCFTAERDLVRASWQKWLVEE
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]