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The map label for this gene is yfhK

Identifier: 30063943

GI number: 30063943

Start: 2668129

End: 2669619

Strand: Reverse

Name: yfhK

Synonym: S2775

Alternate gene names: 30063943

Gene position: 2669619-2668129 (Counterclockwise)

Preceding gene: 30063944

Following gene: 30063942

Centisome position: 58.04

GC content: 52.38

Gene sequence:

>1491_bases
TTGTTGTATCTGAACTTCCCCTCGGTTAGCATCAGGCTATTCGCGTCTGACGAGAGTAACACCTTGAAACGCTGGCCCGT
TTTTCCCCGCTCATTACGACAACTGGTAATGCTGGCATTTTTGCTAATTCTACTACCCCTGTTGGTGCTGGCATGGCAAG
CCTGGCAAAGCCTGAATGCGCTTAGCGATCAGGCGGCGCTGGTTAACCGCACTACGCTTATTGATGCCCGGCGTAGTGAA
GCGATGACCAACGCGGCGCTGGAGATGGAGCGTAGCTACCGTCAGTATTGCGTGCTGGACGACCCTACGCTTGCGAAGGT
TTATCAAAGCCAGCGCAAGCGTTACAGCGAAATGCTCGATGCCCACGCAGGCGTACTGCCAGACGATAAACTTTACCAGG
CATTGCGTCAGGATTTGAACAATCTGGCCCAACTTCAGTGTAACAACAGTGGTCCCGATGCTGCCGCCGCCGCGCGTCTT
GAAGCCTTTGCCAGTGCCAATACCGAAATGGTGCAGGCCACGCGCACAGTGGTGTTCTCTCGTGGGCAGCAACTTCAGCG
TGAAATCGCCGAACGTGGGCAATATTTTGGTTGGCAATCGCTGGTGCTATTTCTGGTGAGTCTGGTGATGGTGCTACTTT
TCACGCGGATGATTATCGGGCCGGTGAAAAATATCGAGCGCATGATCAACCGTCTGGGGGAAGGGCGTTCTCTGGGCAAT
AGCGTTTCGTTCTGTGGACCGAGCGAGTTACGCTCGGTTGGGCAACGTATTCTTTGGTTAAGTGAGCGCCTGTCATGGCT
GGAATCCCAACGCCATCAATTTTTAAGACATTTATCTCATGAATTAAAAACGCCACTGGCGAGTATGCGCGAGGGCACTG
AATTACTGGCTGATCAGGTTGTCGGGCCGCTTACGCCAGAGCAAAAAGAGGTGGTGAGCATTCTTGATAGCAGCAGCTGC
AATTTGCAAAAACTGATCGAACAACTGCTTGATTACAACCGTAAACAGGCGGACAGTGCGGTGGAACTGGAGAATGTTGA
ATTAGCACCGCTGGTGGAGACAGTGGTTTCTGCTCATAGCCTGCCCGCACGGGCTAAAATGATGCATACCGACGTCGATC
TCAAAGCAACAGCTTGCCTGGCGGAGCCAATGCTGCTGATGAGCGTACTGGATAATCTTTACTCCAATGCGGTGCACTAC
GGGGCTGAATCCGGTAACATTTGCCTTCGCAGCAGTTTACATGGTGCGCGGGTTTATATTGATGTCATCAATACAGGCAC
GCCCATTCCGCAAGAGGAACGCGCCATGATCTTCGAACCCTTTTTTCAGGGAAGCCACCAGCGAAAAGGGGCGGTGAAGG
GCAGCGGTCTGGGATTAAGCATTGCCAGGGATTGTATTCGCCGTATGCAAGGGGAACTGTATCTGGTCGACGAGAGCGGG
CAAGACGTTTGTTTCCGCATTGAATTACCGTCGTCGAAAAACACGAAATAA

Upstream 100 bases:

>100_bases
CCGTCCAACTTCTGATATCAGCGTAGCTATATCAACCATCGGGCGAAACGTCGAGTTAGGCACCGCCTTATTCCATAACA
AAGCCGGGTAATTCCCGGCT

Downstream 100 bases:

>100_bases
TGCTAACAGCCTATCCTATCAGGCTATTTTATTTGTCAGTTCGGCCCCGGGTAGTGCTCGAAATCTTCACGTACGATATG
TACGCCCCGGTTTCTCCGCG

Product: putative 2-component sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MLYLNFPSVSIRLFASDESNTLKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSE
AMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARL
EAFASANTEMVQATRTVVFSRGQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGN
SVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSC
NLQKLIEQLLDYNRKQADSAVELENVELAPLVETVVSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHY
GAESGNICLRSSLHGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESG
QDVCFRIELPSSKNTK

Sequences:

>Translated_496_residues
MLYLNFPSVSIRLFASDESNTLKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSE
AMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARL
EAFASANTEMVQATRTVVFSRGQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGN
SVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSC
NLQKLIEQLLDYNRKQADSAVELENVELAPLVETVVSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHY
GAESGNICLRSSLHGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESG
QDVCFRIELPSSKNTK
>Mature_496_residues
MLYLNFPSVSIRLFASDESNTLKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQAALVNRTTLIDARRSE
AMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARL
EAFASANTEMVQATRTVVFSRGQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGN
SVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQVVGPLTPEQKEVVSILDSSSC
NLQKLIEQLLDYNRKQADSAVELENVELAPLVETVVSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHY
GAESGNICLRSSLHGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESG
QDVCFRIELPSSKNTK

Specific function: Probable member of a two-component regulatory system yfhA/yfhK. May activate yfhA by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI87082128, Length=475, Percent_Identity=99.1578947368421, Blast_Score=965, Evalue=0.0,
Organism=Escherichia coli, GI1790861, Length=293, Percent_Identity=28.6689419795222, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI145693157, Length=228, Percent_Identity=29.8245614035088, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI87081816, Length=408, Percent_Identity=25.9803921568627, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789808, Length=291, Percent_Identity=26.1168384879725, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788713, Length=234, Percent_Identity=29.4871794871795, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1789149, Length=354, Percent_Identity=24.2937853107345, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1786912, Length=231, Percent_Identity=28.5714285714286, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=24.1992882562278, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790346, Length=273, Percent_Identity=24.1758241758242, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI48994928, Length=226, Percent_Identity=26.9911504424779, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1790436, Length=219, Percent_Identity=24.6575342465753, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790300, Length=232, Percent_Identity=30.6034482758621, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788549, Length=261, Percent_Identity=25.6704980842912, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786600, Length=231, Percent_Identity=25.974025974026, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1786783, Length=296, Percent_Identity=24.3243243243243, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787894, Length=249, Percent_Identity=26.1044176706827, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788279, Length=246, Percent_Identity=26.4227642276423, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55640; Mature: 55640

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYLNFPSVSIRLFASDESNTLKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNA
CEEEECCCEEEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSDQAALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHH
AHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFS
HHCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
RGQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGN
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
VGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETVVSAHS
CCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCCHHHCCCCHHHHHHHHHHHHC
LPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSLHGARVYI
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEE
DVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESG
EEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCC
QDVCFRIELPSSKNTK
CCEEEEEECCCCCCCC
>Mature Secondary Structure
MLYLNFPSVSIRLFASDESNTLKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNA
CEEEECCCEEEEEEECCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSDQAALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCHHHHHHHHHHHHHHHHHH
AHAGVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFS
HHCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
RGQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLGN
CCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLADQV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
VGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETVVSAHS
CCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCHHHCCCCHHHCCCCHHHHHHHHHHHHC
LPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSLHGARVYI
CCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEE
DVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQGELYLVDESG
EEECCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCC
QDVCFRIELPSSKNTK
CCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]