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The map label for this gene is pepB

Identifier: 30063913

GI number: 30063913

Start: 2633816

End: 2635099

Strand: Reverse

Name: pepB

Synonym: S2742

Alternate gene names: 30063913

Gene position: 2635099-2633816 (Counterclockwise)

Preceding gene: 30063914

Following gene: 30063912

Centisome position: 57.29

GC content: 56.07

Gene sequence:

>1284_bases
ATGACAGAAGCGATGAAGATTACCCTCTCTACCCAACCTGCCGACGCGCGCTGGGGAGAAAAAGCAACTTACAGCATTAA
TAATGACGGCATTACCCTGCATTTGAACGGGGCAGACGATCTGGGGCTGATCCAGCGTGCGGCCCGCAAGATTGACGGTC
TGGGCATCAAGCATGTTCAGTTAAGCGGTGAAGGCTGGGATGCGGATCGCTGCTGGGCATTCTGGCAAGGTTATAAAGGC
CCGAAAGGCACGCGTAAAGTGGAGTGGCCGGATCTGGACGATGCCCAGCGCCAAGAACTGGATAACCGCCTGATGATCAT
CGACTGGGTGCGTGACACCATCAACGCACCGGCAGAAGAATTGGGACCATCGCAACTGGCACAGCGTGCTGTTGATCTGA
TCAGCAACGTCGCGGGCGATCGTGTGACTTATCGGATCACCAAAGGCGAAGATCTGCGTGAGCAAGGTTATATGGGGCTG
CACACTGTCGGGCGCGGTTCAGAACGTTCTCCGGTATTGCTGGCGCTGGATTACAACCCAACTGGCGATAAAGAAGCACC
GGTGTACGCGTGCCTGGTAGGTAAAGGTATCACTTTTGACTCCGGCGGCTACAGCATCAAACAGACTGCGTTTATGGACT
CGATGAAGTCGGACATGGGCGGCGCGGCAACGGTTACCGGGGCGCTGGCATTTGCCATTATGCGCGGACTGAACAAGCGC
GTGAAGCTGTTCCTCTGCTGTGCGGATAACCTGATTAGCGGCAATGCGTTCAAGCTGGGCGATATCATCACCTATCGCAA
CGGTAAAAAAGTTGAAGTGATGAACACTGATGCCGAAGGGCGTCTGGTGCTTGCCGATGGTCTGATTGATGCCAGTGCGC
AGAAACCGGAAATGATCATTGATGCGGCGACCCTCACCGGGGCGGCGAAAACTGCGCTGGGTAATGATTATCACGCGCTG
TTCAGTTTTGACGATGCGCTTGCCGGTCGTTTACTGGCGAGTGCCTCACAAGAGAACGAACCATTCTGGCGTCTGCCGCT
GGCGGAATTCCACCGCAGCCAGCTGCCGTCTAACTTTGCCGAACTGAACAATACCGGAAGCGCGGCGTATCCGGCAGGCG
CGAGCACGGCGGCGGGCTTCCTGTCGCACTTTGTTGAGAACTATCAGCAAGGCTGGCTGCATATCGACTGCTCGGCGACT
TACCGTAAAGCGCCGGTTGAACAGTGGTCTGCGGGCGCTACGGGACTTGGTGTGCGCACGATAGCTAATCTGTTAACGGC
GTAA

Upstream 100 bases:

>100_bases
CTCGAAGCGATTTTGTTAGTCTGGCTGGACGAAGCCGAGTGAATGAACGGGCTGCCTGGGGGTAGCCCGTTTTGCTGTTA
AACGAATAAGGATAACTAAA

Downstream 100 bases:

>100_bases
ACTAATATGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACAAATTCTGCAAATTCAACAAATTGCGAATCCCTTGTAG
GCCTGATAAGCGTAGCGCAT

Product: aminopeptidase B

Products: NA

Alternate protein names: Aminopeptidase B [H]

Number of amino acids: Translated: 427; Mature: 426

Protein sequence:

>427_residues
MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKG
PKGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAIMRGLNKR
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHAL
FSFDDALAGRLLASASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSAT
YRKAPVEQWSAGATGLGVRTIANLLTA

Sequences:

>Translated_427_residues
MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKG
PKGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAIMRGLNKR
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHAL
FSFDDALAGRLLASASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSAT
YRKAPVEQWSAGATGLGVRTIANLLTA
>Mature_426_residues
TEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGP
KGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGLH
TVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAIMRGLNKRV
KLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALF
SFDDALAGRLLASASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSATY
RKAPVEQWSAGATGLGVRTIANLLTA

Specific function: Probably plays an important role in intracellular peptide degradation [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=317, Percent_Identity=36.5930599369085, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI47155554, Length=286, Percent_Identity=35.6643356643357, Blast_Score=137, Evalue=3e-32,
Organism=Escherichia coli, GI87082123, Length=427, Percent_Identity=99.0632318501171, Blast_Score=872, Evalue=0.0,
Organism=Escherichia coli, GI1790710, Length=315, Percent_Identity=38.4126984126984, Blast_Score=187, Evalue=8e-49,
Organism=Caenorhabditis elegans, GI17556903, Length=327, Percent_Identity=32.7217125382263, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17565172, Length=207, Percent_Identity=28.5024154589372, Blast_Score=78, Evalue=8e-15,
Organism=Drosophila melanogaster, GI21357381, Length=344, Percent_Identity=32.2674418604651, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI221379063, Length=344, Percent_Identity=32.2674418604651, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI221379062, Length=344, Percent_Identity=32.2674418604651, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI21355725, Length=291, Percent_Identity=31.6151202749141, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24661038, Length=322, Percent_Identity=30.1242236024845, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24662227, Length=322, Percent_Identity=28.5714285714286, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161077148, Length=344, Percent_Identity=28.4883720930233, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI20130057, Length=344, Percent_Identity=28.4883720930233, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI20129969, Length=294, Percent_Identity=29.2517006802721, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355645, Length=292, Percent_Identity=27.3972602739726, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24662223, Length=292, Percent_Identity=27.3972602739726, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20129963, Length=316, Percent_Identity=27.5316455696203, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI19922386, Length=311, Percent_Identity=27.0096463022508, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24646701, Length=228, Percent_Identity=29.3859649122807, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646703, Length=228, Percent_Identity=29.3859649122807, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21358201, Length=228, Percent_Identity=29.3859649122807, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008330
- InterPro:   IPR011356
- InterPro:   IPR000819 [H]

Pfam domain/function: PF00883 Peptidase_M17 [H]

EC number: =3.4.11.23 [H]

Molecular weight: Translated: 46243; Mature: 46111

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQ
CCCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEE
LSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEE
ECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEHHHHHCCCHHH
LGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGLHTVGRGSERSPVLLALDYNP
CCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHCCCCEEEECCCCCCCCCEEEEEECCC
TGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAIMRGLNKR
CCCCCCCEEEEEECCCEEECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPEMII
EEEEEEECCCCCCCCCEEECCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCCEEE
DAATLTGAAKTALGNDYHALFSFDDALAGRLLASASQENEPFWRLPLAEFHRSQLPSNFA
EEHHHCCHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCHHHH
ELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSATYRKAPVEQWSAGATGLGVRT
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCHHHHCCCCCCCHHHH
IANLLTA
HHHHHCC
>Mature Secondary Structure 
TEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLIQRAARKIDGLGIKHVQ
CCEEEEEEECCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEE
LSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDNRLMIIDWVRDTINAPAEE
ECCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEHHHHHCCCHHH
LGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGLHTVGRGSERSPVLLALDYNP
CCHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHCCCCEEEECCCCCCCCCEEEEEECCC
TGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSDMGGAATVTGALAFAIMRGLNKR
CCCCCCCEEEEEECCCEEECCCCCEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCE
VKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNTDAEGRLVLADGLIDASAQKPEMII
EEEEEEECCCCCCCCCEEECCEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCCEEE
DAATLTGAAKTALGNDYHALFSFDDALAGRLLASASQENEPFWRLPLAEFHRSQLPSNFA
EEHHHCCHHHHHCCCCEEEEEECCHHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCHHHH
ELNNTGSAAYPAGASTAAGFLSHFVENYQQGWLHIDCSATYRKAPVEQWSAGATGLGVRT
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCHHCCCHHHHCCCCCCCHHHH
IANLLTA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]