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The map label for this gene is hyfH

Identifier: 30063864

GI number: 30063864

Start: 2576407

End: 2576952

Strand: Direct

Name: hyfH

Synonym: S2681

Alternate gene names: 30063864

Gene position: 2576407-2576952 (Clockwise)

Preceding gene: 30063863

Following gene: 30063865

Centisome position: 56.02

GC content: 55.31

Gene sequence:

>546_bases
ATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCC
AGGCTTTCGCGGGAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATG
CGCTGACTATCCAGACCGACGACCAGCAAAATTCGCGCACCTGGCAGCTCTATCTGGGGCGTTGTATTTACTGCGGACGT
TGTGAAGAAGTGTGCCCGACCAGAGCCATCCAGCTTACCAATAACTTTGAACTGACCGTCACCCATAAAGCCGATCTCTA
TACCCACGCGACGTTCCATCTACAACGTTGCAGCCGTTGCGAACGCCCGTTTGCCCCGCAAAAAACCGTCGCACTGGCTG
CTGAATTGTTGGCACAGCAACAAAATGCTCCACAAAACCGCGAAATGTTATGGGCGCAAGCGAGCGTCTGCCCGGAGTGC
AAACAACGCGCAACGCTGATCAACGACGATACAGATGTACCGCTGGTGGCTAAGGAGCAGCTATGA

Upstream 100 bases:

>100_bases
GGTAGATGTGCGCAAGCGCCAGTCAAAAACCGTGCTGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGC
CGCTGAAGTAAGGACAGAAG

Downstream 100 bases:

>100_bases
GTCCAGTGCTTACGCAATATGTCAGCCAGCCCATCACGCTGGACGAGCAAACGCAAAAGATGAAGCGGCATTTGCTACAG
GATATCCGTCGCTCGGCTTA

Product: hydrogenase 4 subunit H

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGR
CEEVCPTRAIQLTNNFELTVTHKADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
KQRATLINDDTDVPLVAKEQL

Sequences:

>Translated_181_residues
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGR
CEEVCPTRAIQLTNNFELTVTHKADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
KQRATLINDDTDVPLVAKEQL
>Mature_181_residues
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGR
CEEVCPTRAIQLTNNFELTVTHKADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
KQRATLINDDTDVPLVAKEQL

Specific function: Probable electron transfer protein for hydrogenase 4 [H]

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=105, Percent_Identity=41.9047619047619, Blast_Score=74, Evalue=6e-14,
Organism=Escherichia coli, GI1788833, Length=181, Percent_Identity=97.7900552486188, Blast_Score=329, Evalue=5e-92,
Organism=Escherichia coli, GI1789075, Length=170, Percent_Identity=44.7058823529412, Blast_Score=156, Evalue=8e-40,
Organism=Escherichia coli, GI1788617, Length=106, Percent_Identity=37.7358490566038, Blast_Score=74, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17555194, Length=105, Percent_Identity=40.9523809523809, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI17864306, Length=105, Percent_Identity=41.9047619047619, Blast_Score=75, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: NA

Molecular weight: Translated: 20133; Mature: 20133

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

7.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
9.4 %Cys+Met (Translated Protein)
7.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
9.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACACPANALTIQTD
CHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHCCHHHCCCCCEEEEEEC
DQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTHKADLYTHATFHLQRCSRC
CCCCCEEEEEEECCEEECCCCHHHCCCCEEEEECCEEEEEEECCCEEHHHHHHHHHHHHC
ERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQRATLINDDTDVPLVAKEQ
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHCCEECCCCCCCCEEECCC
L
C
>Mature Secondary Structure
MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACGACACACPANALTIQTD
CHHHHHHHHHCCCCEEECCCCCEEECCCCCCCCCCCHHHHHHHCCHHHCCCCCEEEEEEC
DQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTHKADLYTHATFHLQRCSRC
CCCCCEEEEEEECCEEECCCCHHHCCCCEEEEECCEEEEEEECCCEEHHHHHHHHHHHHC
ERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQRATLINDDTDVPLVAKEQ
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHCCEECCCCCCCCEEECCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]