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The map label for this gene is ypfI

Identifier: 30063853

GI number: 30063853

Start: 2560035

End: 2562050

Strand: Reverse

Name: ypfI

Synonym: S2667

Alternate gene names: 30063853

Gene position: 2562050-2560035 (Counterclockwise)

Preceding gene: 30063854

Following gene: 30063852

Centisome position: 55.7

GC content: 55.16

Gene sequence:

>2016_bases
ATGGCTGAACTGACTGCGCTTTACACATTAACAGCGCAAATGAAACGTGAAGGGATCTGCCGCTTGCTGGTGTTGAGCGG
GGAAGAGGGTTGGTGTTTTGATCATGCTCTTAAGTTGCGTGATGCCTTACCTGGCGACTGGCTGTGGATTTCGCCGCAGC
TAGATGCTGAAAACCACTGTTCACCCTCGGCGCTACAAACTTTACTTGGGCGCGAGTTCCGGCATGCGGTATTCGACGCC
CGCCACGGCTTTGATGCCGCTGCCTTTGCAGCACTTAGCGGAACGTTGAAAGCGGGAAGCTGGCTGGTTTTGTTACTCCC
TGTATGGGAAGAGTGGGAAAACCAACCTGATGCCGACTCGCTGCGCTGGAGTGATTGCCCTGACCCTATTGCGACGCCGC
ATTTTGTCCAGCATCTCAAACGCGTACTTACGGCGGATAACGACGCTATCCTCTGGCGGCAAAACCAGCCGTTCTCGTTG
GCGCATTTTACTCCCCGTACTGACTGGCACCCCGCGACCGGCGCACCACAACCAGAACAACAGCAACTCTTACAGCAGCT
ACTGACCATGCCGCCGGGCGTGGCAGCGGTAACGGCTGCGCGTGGGCGCGGTAAGTCGGCGTTGGCAGGGCAACTCATTT
CTCGTATTGCGGGCAGTGCGATTGTCACCGCGCCCGCAAAAGCGGCAACGGATGTACTGGCACAATTTGCGGGCGAGAAG
TTTCGCTTTATTGCACCGGATGCCTTGTTAGCCAGCGATGAGCAAGCCGACTGGCTGGTGGTCGACGAAGCCGCAGCCAT
ACCTGCGCCGTTGTTGCATCAACTGGTATCGCGTTTTCCTCGAACGTTGTTAACCACTACGGTGCAGGGCTACGAAGGTA
CCGGACGTGGTTTTTTGCTGAAATTTTGCGCTCGCTTTCCGCATTTACACCGTTTTGAACTGCAACAGCCGATCCGCTGG
GCACAGGGATGCCCGCTGGAAAAAATGGTTAGTGAGGCACTGGTTTTTAACGATGAAAACTTCACCCATACACCACAAGG
CAATATCGTCATTTCCGCATTTGAACAAACGTTATGGCGAAGCGAGCCAGAAACGCCGTTAAAGGTTTATCAGTTATTGT
CTGGTGCGCACTACCGGACCTCGCCGCTGGATTTACGCCGGATGATGGATGCACCAGGGCAACATTTTTTACAGGCGGCT
GGCGAAAACGAGATTGCCGGGGCGTTGTGGCTGGTGGATGAGGGCGGATTATCTCAAGAACTCAGTCAGGCGGTATGGGC
AGGTTTTCGTCGCCCGCGGGGTAATCTGGTGGCCCAGTCGCTGGCGGCGCACGGCAGCAATCCACTGGCGGCGACATTGC
GCGGAAGGCGGGTTAGCCGGATAGCAGTTCATCCGGCTCGTCAGCGGGAAGGCACAGGGCGACAACTTATTGCTGGAGCT
TTGCAATATACGCATGACCTCGACTATCTTTCGGTGAGTTTTGGTTACACTGGGGAGTTATGGCGTTTCTGGCAACGCTG
CGGTTTTGTGCTGGTGCGGATGGGTAATCATCGGGAAGCCAGCAGCGGTTGCTATACGGCGATGGCGCTGTTACCGATGA
GTGATGCGGGTAAACAGCTGGCTGAACGTGAGCATTACCGTTTACGTCGCGATGCGCAAGCTCTCGCGCAGTGGAATGGC
GAAATGCTTCCTGTTGATCCACTAAACGATGCCATCCTTTCTGACGACGACTGGCTTGAACTGGCCGGTTTTGCTTTCGC
TCATCGTCCGCTATTAACATCGTTAGGTTGCTTATTGCGTCTGCTACAAACCAGTGAACTGGCATTACCGGCGCTGCGTG
GGCGTTTACAGAAAAACGCCAGTGACGCGCAGTTATGTACCACACTTAAACTTTCAGGCCGCAAGATGTTACTGGTTCGC
CAGCGGGAAGAGGCCGCGCAGGCGCTGTTCGCACTAAATGAGGTTCGCACTGAGCGTCTGCGCGATCGCATAACGCAATG
GCAATTTTTTCACTGA

Upstream 100 bases:

>100_bases
CTTCTCAGCAACGCTACAGCTGGTTTAAACGTGGTTTCGACAGCGGCGATCCGGCACAATGCAATACTTTTGGTAAAAGC
ATTTAATTTCCGGGGCAGGG

Downstream 100 bases:

>100_bases
CTCCTTCAATTTTCTCCCATGGTCATTGTGAGTCAGCGGCGTAAAATTAATGACATCAGTTAAGGAGTTCGCCATGAAAC
ATGACCATTTTGTTGTTCAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 670

Protein sequence:

>671_residues
MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDA
RHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEK
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRW
AQGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAA
GENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIAGA
LQYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNG
EMLPVDPLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQLCTTLKLSGRKMLLVR
QREEAAQALFALNEVRTERLRDRITQWQFFH

Sequences:

>Translated_671_residues
MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDA
RHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEK
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRW
AQGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAA
GENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIAGA
LQYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNG
EMLPVDPLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQLCTTLKLSGRKMLLVR
QREEAAQALFALNEVRTERLRDRITQWQFFH
>Mature_670_residues
AELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDAR
HGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSLA
HFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEKF
RFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWA
QGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAAG
ENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIAGAL
QYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGE
MLPVDPLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQLCTTLKLSGRKMLLVRQ
REEAAQALFALNEVRTERLRDRITQWQFFH

Specific function: Catalyzes the formation of N(4)-acetylcytidine (ac(4)C) at the wobble position of tRNA(Met), by using acetyl-CoA as an acetyl donor and ATP (or GTP). It recognizes the wobble base of tRNA(Met), thus distinguishing between tRNA(Met) and the structurally si

COG id: COG1444

COG function: function code R; Predicted P-loop ATPase fused to an acetyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788818, Length=671, Percent_Identity=96.7213114754098, Blast_Score=1323, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17507621, Length=367, Percent_Identity=25.3405994550409, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324197, Length=379, Percent_Identity=25.8575197889182, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24640662, Length=455, Percent_Identity=25.0549450549451, Blast_Score=100, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181
- InterPro:   IPR013562
- InterPro:   IPR007807 [H]

Pfam domain/function: PF08351 DUF1726; PF05127 DUF699 [H]

EC number: NA

Molecular weight: Translated: 74783; Mature: 74652

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQLDAENHC
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCC
SPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADS
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCC
LRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSLAHFTPRTDWHPATGAPQPEQ
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCHHH
QQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEK
HHHHHHHHCCCCCHHEEHHHCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCC
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLL
EEEECCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KFCARFPHLHRFELQQPIRWAQGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWR
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCEEEEHHHHHHHC
SEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAAGENEIAGALWLVDEGGLSQE
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEECCCCHHH
LSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIAGA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEHHCCCEEEEEEECCHHHCCCCCHHHHHHH
LQYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQL
HHHHCCCCEEEEECCCCHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
AEREHYRLRRDAQALAQWNGEMLPVDPLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLR
HHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTSELALPALRGRLQKNASDAQLCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHEEECCCEEEEEECHHHHHHHHHHHHHHHHHHH
RDRITQWQFFH
HHHHHHCCCCC
>Mature Secondary Structure 
AELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALPGDWLWISPQLDAENHC
CCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCC
SPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWLVLLLPVWEEWENQPDADS
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCEEEEEEECHHHHCCCCCCCC
LRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSLAHFTPRTDWHPATGAPQPEQ
CCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCHHH
QQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAGSAIVTAPAKAATDVLAQFAGEK
HHHHHHHHCCCCCHHEEHHHCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHHHHCCCC
FRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLVSRFPRTLLTTTVQGYEGTGRGFLL
EEEECCCHHHCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KFCARFPHLHRFELQQPIRWAQGCPLEKMVSEALVFNDENFTHTPQGNIVISAFEQTLWR
HHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHEECCCCCCCCCCCCEEEEHHHHHHHC
SEPETPLKVYQLLSGAHYRTSPLDLRRMMDAPGQHFLQAAGENEIAGALWLVDEGGLSQE
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCEEEEEEECCCCHHH
LSQAVWAGFRRPRGNLVAQSLAAHGSNPLAATLRGRRVSRIAVHPARQREGTGRQLIAGA
HHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEHHCCCEEEEEEECCHHHCCCCCHHHHHHH
LQYTHDLDYLSVSFGYTGELWRFWQRCGFVLVRMGNHREASSGCYTAMALLPMSDAGKQL
HHHHCCCCEEEEECCCCHHHHHHHHHHCEEEEEECCCCCCCCCHHHHHHHCCCCHHHHHH
AEREHYRLRRDAQALAQWNGEMLPVDPLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLR
HHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
LLQTSELALPALRGRLQKNASDAQLCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHEEECCCEEEEEECHHHHHHHHHHHHHHHHHHH
RDRITQWQFFH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]