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The map label for this gene is xasA [C]

Identifier: 30063838

GI number: 30063838

Start: 2536123

End: 2537583

Strand: Direct

Name: xasA [C]

Synonym: S2649

Alternate gene names: 30063838

Gene position: 2536123-2537583 (Clockwise)

Preceding gene: 30063837

Following gene: 30063844

Centisome position: 55.14

GC content: 39.9

Gene sequence:

>1461_bases
ATGTCTAATGCTAATATCGCACCCAAAAGTAAGAAAATGACAGTGGGGACACTCGCTATCATGAATATAACTGCTGTAGT
AAGTCTACGATTTATACCTTCTGAAGCAGAATACGGCTTAGGTGCCATTTTTTACTATACCTTTGCTGCAATTATGTTTT
TAGTACCTGTTGCCTTTGTTGCGGCTGAGCTAGCTACAACATATCCACAAAAAGGGGGGGCATTTCGATGGATAGGAGAA
GCATTTGGGGCTCGCTGGGGTTTTGTAGGAATGCTTATGACCTGGCTACAAGTCATCCCCTACTTTCCAACAGTACTGAC
ATTTGGTGCAGTCTCGGTTGCATTTATTGATGTTGATATGGGGGTGGCAGAATCAATTGCCAGTAATAAATGGTATATTT
TATTTTTCGTACTATTTGTTTACTGGGGAGCTATTTGTGTTGCATTACGTGGCGTTGGTATTTTCTCGCAGGTATCTAAG
TGGTGCGGTATTATAGGGACAATTATTCCAGCTATTATCCTTGTTATTCTTGGTTTCTCCTATCTGTGGTTCAGCGGTAA
ACCAGCGGTGATCCCATTAGGTTGGGGTGATCTTTTCCCTAATATAGCTAACTTCCAGAATATTGTTCTTGCCGCCAGCA
TATTTCTTGCTTATGCAGGGATGGAAATGAATGCGGTCCATGTTAACGATTTAGATAATCCAACTAAAAAATACCCAATT
GCAATTACCATCGCTTCTATAGGTACTATTGCAATATTCCTGCTCAGTACTTTAGGGGTCGCCTTTATTATTCCTACAGA
TAAAATTAGTCTAACTCAAAGTTTGTTGGTTGCCTACGATATGCTGTTCGAATGGGCTGGAGTGCCATGGCTTGGCTCTG
TGATGGCATTTATGCTAGCAATTGGCGTACTGGGTGGGGTGGTTACTTGGATTGCAGGGCCTAATACAGGTGTATTAGCG
ATCGCGAAAGCTGGTTATTTACCTAAGTTTTTCCAAAAAACCAATCGTCATGGAATGGGGCACCATCTAATGTTTGTACA
AGGTATAATTGTCTCAGTTCTTTCTGTTACTTTTGTGATAATGCCTTCAGTTCAAGCAGCTTTTCAGATTTTATCACAGT
TAACAGTTATGCTCTATTTAGTTATGTATATGCTCATGTTCGCTAGTGCTATCTATCTACGACATTCTCAACCTGATACA
AAACGCCCATATCGAGCCCCTGCTTTATTTTTCTGGGCAACACTTGGATTCTTAGGGTCTTTTCTGGCTTTTGTATTAAG
TTTTATTCCGCCCGAGCAAATCCCAACTGGTAGTCCAACCAGCTATGTTCTTTATTTGTTAGTATTAGTTGCCCTATTTT
TATTAATTCCAATATTAATTTACGCCTTCCGAAAATCTGACTGGAAAGATCCAAACTCTGATTTCGAACCATTTTCTTGG
GAAAAAAGAACTAGTAATTAA

Upstream 100 bases:

>100_bases
TGCGTTAGTGTTTTTAAGATAAGTGTTAAAGTTAATATTAGTTTTTATATTTACATCGTAATTATATGTTTCTATTTTAA
AAATGTTCGAGAGTATAAAA

Downstream 100 bases:

>100_bases
ATTTTCCATAAGCTACAAAAGATGAGGTTAAATATGTTTAGTGCTGAATCTCCAATTGGTGTAATAGACTCAGGTGTCGG
TGGATTGACATCGGTAAAGG

Product: putative amino acid antiporter

Products: L-glutamate [Cytoplasm]; 4-aminobutyrate [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIFYYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGE
AFGARWGFVGMLMTWLQVIPYFPTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAYAGMEMNAVHVNDLDNPTKKYPI
AITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLLVAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLA
IAKAGYLPKFFQKTNRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIYLRHSQPDT
KRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLVALFLLIPILIYAFRKSDWKDPNSDFEPFSW
EKRTSN

Sequences:

>Translated_486_residues
MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIFYYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGE
AFGARWGFVGMLMTWLQVIPYFPTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAYAGMEMNAVHVNDLDNPTKKYPI
AITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLLVAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLA
IAKAGYLPKFFQKTNRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIYLRHSQPDT
KRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLVALFLLIPILIYAFRKSDWKDPNSDFEPFSW
EKRTSN
>Mature_485_residues
SNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIFYYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEA
FGARWGFVGMLMTWLQVIPYFPTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSKW
CGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAYAGMEMNAVHVNDLDNPTKKYPIA
ITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLLVAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAI
AKAGYLPKFFQKTNRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIYLRHSQPDTK
RPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLVALFLLIPILIYAFRKSDWKDPNSDFEPFSWE
KRTSN

Specific function: Probable Amino Acid Transporter. Involved In Extreme Acid Resistance. [C]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1787768, Length=492, Percent_Identity=28.0487804878049, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1786908, Length=278, Percent_Identity=25.5395683453237, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 53469; Mature: 53338

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIFYYTFAAIMFLVPVAFV
CCCCCCCCCCCCEEHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYFPTVLTFGAVSVAFIDVDM
HHHHHHHCCCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECH
GVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSKWCGIIGTIIPAIILVILGFS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAYAGMEMNAVHVNDLDNPTKKYPI
HHHCCCCCCEEECCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCE
AITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLLVAYDMLFEWAGVPWLGSVMAFMLA
EEEHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKTNRHGMGHHLMFVQGIIVSVLSVTFVI
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
MPSVQAAFQILSQLTVMLYLVMYMLMFASAIYLRHSQPDTKRPYRAPALFFWATLGFLGS
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
FLAFVLSFIPPEQIPTGSPTSYVLYLLVLVALFLLIPILIYAFRKSDWKDPNSDFEPFSW
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EKRTSN
CCCCCC
>Mature Secondary Structure 
SNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIFYYTFAAIMFLVPVAFV
CCCCCCCCCCCEEHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYFPTVLTFGAVSVAFIDVDM
HHHHHHHCCCCCCCCEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECH
GVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSKWCGIIGTIIPAIILVILGFS
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAYAGMEMNAVHVNDLDNPTKKYPI
HHHCCCCCCEEECCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCE
AITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLLVAYDMLFEWAGVPWLGSVMAFMLA
EEEHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
IGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKTNRHGMGHHLMFVQGIIVSVLSVTFVI
HHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
MPSVQAAFQILSQLTVMLYLVMYMLMFASAIYLRHSQPDTKRPYRAPALFFWATLGFLGS
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
FLAFVLSFIPPEQIPTGSPTSYVLYLLVLVALFLLIPILIYAFRKSDWKDPNSDFEPFSW
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
EKRTSN
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-aminobutyrate [Cytoplasm]; L-glutamate [Periplasm] [C]

Specific reaction: 4-aminobutyrate [Cytoplasm] + L-glutamate [Periplasm] = L-glutamate [Cytoplasm] + 4-aminobutyrate [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8366052 [H]