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The map label for this gene is cysW

Identifier: 30063816

GI number: 30063816

Start: 2516241

End: 2516690

Strand: Reverse

Name: cysW

Synonym: S2624

Alternate gene names: 30063816

Gene position: 2516690-2516241 (Counterclockwise)

Preceding gene: 30063817

Following gene: 30063815

Centisome position: 54.72

GC content: 57.33

Gene sequence:

>450_bases
ATGTTCTCCTGGCCCGGAATGGTGCTGGTCACCATCTTTGTGACGTGTCCGTTTGTGGTGCGCGAGCTGGTGCCGGTGAT
GTTAAGCCAGGGCAGCCAGGAAGACGAAGCGGCGATTTTGCTTGGCGCGTCCGGCTGGCAGATGTTCCGTCGCGTCACAT
TGCCGAATATTCGCTGGGCGCTGCTTTACGGCGTGGTGCTGACCAACGCCCGCGCGATTGGCGAGTTTGGTGCAGTGTCG
GTGGTTTCCGGCTCGATTCGCGGCGAAACACTTTCGCTGCCGTTACAGATTGAATTGTTGGAGCAGGACTACAACACCGT
CGGCTCCTTTACCGCCGCCGCGCTGTTGACGTTGATGGCGATTATCACCCTGTTTTTAAAGAGTATGTTGCAGTGGCGCC
TGGAGAATCAGGAAAAACGCGCGCAGCAGGAGGAGCATCATGAGCATTGA

Upstream 100 bases:

>100_bases
GTTTGCTGTATCGCCGGTGGTTGCCGGTCTGGTGTATCTGCTGTTCTACGGCTCCAACGGCCCGCTCGGCGGCTGGCTGG
ATGAACATAATCTGCAAATT

Downstream 100 bases:

>100_bases
GATTGCCAATATCAAGAAGTCTTTTGGTCGCACCCAGGTGCTGAACGATATCTCACTGGATATTCCTTCTGGTCAGATGG
TCGCGTTGCTGGGGCCGTCC

Product: sulfate transport system permease W protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MFSWPGMVLVTIFVTCPFVVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEFGAVS
VVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRLENQEKRAQQEEHHEH

Sequences:

>Translated_149_residues
MFSWPGMVLVTIFVTCPFVVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEFGAVS
VVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRLENQEKRAQQEEHHEH
>Mature_149_residues
MFSWPGMVLVTIFVTCPFVVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEFGAVS
VVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRLENQEKRAQQEEHHEH

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI87082099, Length=149, Percent_Identity=100, Blast_Score=302, Evalue=7e-84,
Organism=Escherichia coli, GI1788764, Length=105, Percent_Identity=41.9047619047619, Blast_Score=80, Evalue=5e-17,
Organism=Escherichia coli, GI1786980, Length=144, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSW_ECOL6 (P0AEB1)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_754839.1
- ProteinModelPortal:   P0AEB1
- SMR:   P0AEB1
- EnsemblBacteria:   EBESCT00000041609
- GeneID:   1038593
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c2957
- GeneTree:   EBGT00050000008926
- HOGENOM:   HBG685300
- OMA:   LQIELLY
- ProtClustDB:   PRK11602
- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667
- TIGRFAMs:   TIGR00969
- TIGRFAMs:   TIGR02140

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 16676; Mature: 16676

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1400063c)-; HASH(0x141a5d80)-; HASH(0x1403e038)-; HASH(0x141b1428)-; HASH(0x11b75934)-; HASH(0x13c67900)-;

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSWPGMVLVTIFVTCPFVVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHCCCCHHHH
LLYGVVLTNARAIGEFGAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMA
HHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
IITLFLKSMLQWRLENQEKRAQQEEHHEH
HHHHHHHHHHHHHHCCHHHHHHHHHCCCH
>Mature Secondary Structure
MFSWPGMVLVTIFVTCPFVVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHHHHHCCCCHHHH
LLYGVVLTNARAIGEFGAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMA
HHHHHHHHHHHHHHCCCCHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
IITLFLKSMLQWRLENQEKRAQQEEHHEH
HHHHHHHHHHHHHHCCHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157