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The map label for this gene is pdxK

Identifier: 30063812

GI number: 30063812

Start: 2511819

End: 2512670

Strand: Reverse

Name: pdxK

Synonym: S2619

Alternate gene names: 30063812

Gene position: 2512670-2511819 (Counterclockwise)

Preceding gene: 30063814

Following gene: 30063806

Centisome position: 54.63

GC content: 51.88

Gene sequence:

>852_bases
ATGAGTAGTTTGTTGTTGTTTAACGATAAGAGTAGGGCGCTGCAGGCGGATATCGTCGCCGTGCAGTCGCAGGTAGTGTA
CGGAAGCGTAGGCAACAGCATTGCGGTGCCTGCTATAAAACAGAACGGCTTGAATGTCTTTGCCGTGCCGACGGTATTGC
TGAGCAATACGCCGCATTATGACACTTTCTACGGTGGTGCGATTCCGGACGAATGGTTTAGCGGCTATTTGCGTGCGCTT
CAGGAGCGTGATGCGCTGCGCCAACTTCGTGCTGTAACCACGGGCTATATGGGAACGGCATCGCAAATCAAAATCCTTGC
CGAGTGGCTGACTGCGCTACGCAAAGACCATCCTGACCTATTGATCATGGTCGATCCGGTGATTGGCGATATTGATAGCG
GAATTTATGTCAAACCTGACCTTCCCGAGGCGTATCGACAATATTTACTGCCGCTGGCGCAGGGAATTACCCCCAATATC
TTTGAGCTGGAAATCCTGACCGGTAAAAATTGCCGCGATCTCGACAGTGCCATTGCTGCCGCAAAAAGTCTGCTTTCAGA
CACATTAAAATGGGTGGTGATTACCAGCGCCTCCGGTAATGAAGAAAATCAGGAGATGCAGGTGGTGGTGGTCAGTGCCG
ACAGCGTGAATGTCATTTCCCATTCACGGGTAAAAACCGACCTGAAAGGGACTGGCGACCTGTTTTGTGCTCAGCTTATC
AGTGGTTTGCTGAAAGGGAAGGCGTTAAACGATGCGGTGCACCGTGCGGGGTTGCGCGTACTGGAAGTGATGCGCTACAC
CCAGCAGCATGAGAGCGATGAATTGATTTTGCCGCCGCTGGCGGAAGCATAA

Upstream 100 bases:

>100_bases
TTACCTTATCTTTGTTTTGTAATAGCCGATTTGACTGCCAGAATCAGGTAAAGTTTCGGTTGGAATACGATGAGGATCAT
CGGCAAGGAGAAAAAATTAA

Downstream 100 bases:

>100_bases
AAAATGGCGCCGATGGGCGCCATTTTTCACTGCGGCAAGAATTACTTCTTGATGCGGATAACCGGGGTTTCACCCACGGT
TACGCTACCGGACAGTTTGA

Product: pyridoxal kinase

Products: NA

Alternate protein names: PN/PL/PM kinase; Pyridoxal kinase; Pyridoxamine kinase; Vitamin B6 kinase

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGLNVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRAL
QERDALRQLRAVTTGYMGTASQIKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNVISHSRVKTDLKGTGDLFCAQLI
SGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELILPPLAEA

Sequences:

>Translated_283_residues
MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGLNVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRAL
QERDALRQLRAVTTGYMGTASQIKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNVISHSRVKTDLKGTGDLFCAQLI
SGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELILPPLAEA
>Mature_282_residues
SSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGLNVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQ
ERDALRQLRAVTTGYMGTASQIKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNIF
ELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNVISHSRVKTDLKGTGDLFCAQLIS
GLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELILPPLAEA

Specific function: Phosphorylates B6 vitamers; functions in a salvage pathway. Uses pyridoxal, pyridoxine, and pyridoxamine as substrates

COG id: COG2240

COG function: function code H; Pyridoxal/pyridoxine/pyridoxamine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyridoxine kinase family

Homologues:

Organism=Homo sapiens, GI4505701, Length=241, Percent_Identity=27.8008298755187, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1788758, Length=283, Percent_Identity=98.9399293286219, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1787924, Length=266, Percent_Identity=28.9473684210526, Blast_Score=118, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17507759, Length=268, Percent_Identity=29.4776119402985, Blast_Score=100, Evalue=7e-22,
Organism=Caenorhabditis elegans, GI17507757, Length=279, Percent_Identity=28.673835125448, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320806, Length=236, Percent_Identity=27.1186440677966, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324354, Length=239, Percent_Identity=27.6150627615063, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI45553007, Length=188, Percent_Identity=34.0425531914894, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXK_ECO24 (A7ZPL9)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001463748.1
- ProteinModelPortal:   A7ZPL9
- SMR:   A7ZPL9
- STRING:   A7ZPL9
- EnsemblBacteria:   EBESCT00000019594
- GeneID:   5588451
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_2705
- eggNOG:   COG2240
- GeneTree:   EBGT00050000009567
- HOGENOM:   HBG661459
- OMA:   TRDASFL
- ProtClustDB:   PRK08176
- BioCyc:   ECOL331111:ECE24377A_2705-MONOMER
- HAMAP:   MF_01638
- InterPro:   IPR013749
- InterPro:   IPR004625
- TIGRFAMs:   TIGR00687

Pfam domain/function: PF08543 Phos_pyr_kin

EC number: =2.7.1.35

Molecular weight: Translated: 30861; Mature: 30730

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: BINDING 23-23 BINDING 136-136 BINDING 233-233

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGLNVFAVPTVLLSNTPHY
CCCEEEECCCCHHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCEEEEEHHHHHCCCCCC
DTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQIKILAEWLTALRKDHPDL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCE
LIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNIFELEILTGKNCRDLDSAIAA
EEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
AKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNVISHSRVKTDLKGTGDLFCAQLI
HHHHHHHCEEEEEEECCCCCCCCCEEEEEEEECCCEEEHHHHHHHCCCCCCHHHHHHHHH
SGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELILPPLAEA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC
>Mature Secondary Structure 
SSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGLNVFAVPTVLLSNTPHY
CCEEEECCCCHHHHHHHHHHHHHHEEECCCCCEECCCCCCCCCEEEEEHHHHHCCCCCC
DTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQIKILAEWLTALRKDHPDL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCE
LIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNIFELEILTGKNCRDLDSAIAA
EEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHH
AKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNVISHSRVKTDLKGTGDLFCAQLI
HHHHHHHCEEEEEEECCCCCCCCCEEEEEEEECCCEEEHHHHHHHCCCCCCHHHHHHHHH
SGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELILPPLAEA
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA