Click here to switch to the map view.

The map label for this gene is nupC

Identifier: 30063797

GI number: 30063797

Start: 2494019

End: 2495221

Strand: Direct

Name: nupC

Synonym: S2598

Alternate gene names: 30063797

Gene position: 2494019-2495221 (Clockwise)

Preceding gene: 30063794

Following gene: 30063799

Centisome position: 54.23

GC content: 49.46

Gene sequence:

>1203_bases
ATGGACCGCGTCCTTCATTTTGTACTGGCACTTGCCGTTGTTGTGATTCTCGCACTGCTGGTAAGCAGCGACCGCAAAAA
AATTCGTATCCGTTATGTTATTCAACTGCTTGTTATCGAAGTGTTACTGGCGTGGTTCTTCCTGAACTCCGACGTTGGTT
TAGGCTTCGTGAAAGGCTTCTCCGAAATGTTCGAAAAACTGCTCGGATTTGCCAACGAAGGGACTAACTTCGTCTTTGGT
AGCATGAATGATCAAGGCCTGGCATTCTTCTTCCTGAAAGTGCTGTGCCCAATCGTCTTTATCTCTGCACTGATCGGTAT
TCTCCAGCACATTCGCGTGTTGCCGGTGATTATCCGCGCAATTGGTTTCCTGCTCTCCAAAGTCAACGGTATGGGCAAAC
TGGAATCCTTTAACGCCGTCAGCTCCCTGATTCTGGGTCAGTCAGAAAACTTTATTGCCTATAAAGATATCCTCGGCAAA
ATCTCCCGTAATCGTATGTACACCATGGCTGCCACGGCAATGTCCACCGTGTCGATGTCCATCGTTGGTGCATACATGAC
CATGCTGGAACCGAAATACGTCGTTGCTGCGCTGGTACTGAACATGTTCAGCACCTTTATCGTGCTGTCGCTGATCAATC
CTTACCGTGTTGATGCCAGTGAAGAAAACATCCAGATGTCCAACCTGCACGAAGGTCAGAGCTTCTTCGAAATGCTGGGT
GAATACATTCTGGCAGGTTTCAAAGTTGCCATTATCGTTGCCGCGATGCTGATCGGCTTTATCGCCCTGATCGCCGCGCT
GAACGCACTGTTTGCCACCGTTACTGGCTGGTTTGGCTACAGCATCTCCTTCCAGGGCATCCTGGGCTACATCTTCTATC
CGATTGCATGGGTGATAGGTGTTCCTTCCAGTGAAGCACTGCAAGTGGGCAGTATCATGGCGACCAAACTGGTTTCCAAC
GAGTTCGTTGCGATGATGGATCTGCAGAAAATTGCTTCCACGCTCTCTCCGCGTGCTGAAGGCATCATCTCTGTGTTCCT
AGTTTCCTTCGCTAACTTCTCTTCAATCGGGATTATCGCAGGTGCAGTTAAAGGCCTGAATGAAGAGCAAGGTAACGTGG
TTTCTCGCTTCGGTCTGAAACTGGTTTACGGCTCTACCCTGGTGAGTGTGCTGTCTGCGTCAATCGCAGCACTGGTGCTG
TAA

Upstream 100 bases:

>100_bases
TCACAGGACGTCATTATAGTGTGTGTCAGATCTCGTTTTCCTTAACCATGTTACATAGAATGTGCACGGAAATTTAACCT
GCCTCATATTTGGAGCAAAT

Downstream 100 bases:

>100_bases
AACCATACATAAAAAAGCCGGGGATCATTCCCCGGCTTTTTTACGCCTGTTAATCAGCTAATGGCTGCGGGCGACCAATC
AAATACCCTTGCAGATATTG

Product: permease of transport system for 3 nucleosides

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: Nucleoside-transport system protein nupC [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFG
SMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMSNLHEGQSFFEMLG
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVIGVPSSEALQVGSIMATKLVSN
EFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL

Sequences:

>Translated_400_residues
MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFG
SMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMSNLHEGQSFFEMLG
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVIGVPSSEALQVGSIMATKLVSN
EFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL
>Mature_400_residues
MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFG
SMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMSNLHEGQSFFEMLG
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVIGVPSSEALQVGSIMATKLVSN
EFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL

Specific function: Transports nucleosides with a high affinity except guanosine and deoxyguanosine. Driven by a proton motive force [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=414, Percent_Identity=25.1207729468599, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI11545853, Length=411, Percent_Identity=23.8442822384428, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI227116277, Length=413, Percent_Identity=21.3075060532688, Blast_Score=93, Evalue=5e-19,
Organism=Escherichia coli, GI1788737, Length=400, Percent_Identity=99.5, Blast_Score=783, Evalue=0.0,
Organism=Escherichia coli, GI1788485, Length=414, Percent_Identity=31.8840579710145, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1788488, Length=413, Percent_Identity=30.2663438256659, Blast_Score=160, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17560276, Length=393, Percent_Identity=24.1730279898219, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71991794, Length=399, Percent_Identity=24.5614035087719, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI45552519, Length=405, Percent_Identity=24.9382716049383, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI45552517, Length=405, Percent_Identity=24.9382716049383, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI19921868, Length=405, Percent_Identity=24.9382716049383, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI281360430, Length=344, Percent_Identity=24.4186046511628, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 43486; Mature: 43486

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGF
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRA
HHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKISRNRMYTMAATAMSTVSMS
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMSNLHEGQSFFEMLG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHH
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHC
VPSSEALQVGSIMATKLVSNEFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHH
GAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLAWFFLNSDVGLGFVKGF
CHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
SEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFISALIGILQHIRVLPVIIRA
HHHHHHHHCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGKISRNRMYTMAATAMSTVSMS
HHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
IVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVDASEENIQMSNLHEGQSFFEMLG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCHHHHHHHHH
EYILAGFKVAIIVAAMLIGFIALIAALNALFATVTGWFGYSISFQGILGYIFYPIAWVIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHC
VPSSEALQVGSIMATKLVSNEFVAMMDLQKIASTLSPRAEGIISVFLVSFANFSSIGIIA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHH
GAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLSASIAALVL
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]