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The map label for this gene is fadI

Identifier: 30063763

GI number: 30063763

Start: 2447579

End: 2448889

Strand: Reverse

Name: fadI

Synonym: S2555

Alternate gene names: 30063763

Gene position: 2448889-2447579 (Counterclockwise)

Preceding gene: 30063764

Following gene: 30063762

Centisome position: 53.24

GC content: 55.53

Gene sequence:

>1311_bases
ATGGGTCAGGTTTTACCACTGGTTACCCGCCAGGGCGATCGTATCGCCATTGTTAGCGGTTTACGTACGCCTTTTGCCCG
CCAGGCGACGGCTTTTCATGGCATTCCCACGGTTGATTTAGGGAAGATGGTGGTAGGCGAACTGCTGGCACGCAGCGAGA
TCCCCGCTGAAGTGATTGAACAGCTGGTCTTTGGTCAGGTCGTACAAATGCCTGAAGCCCCCAACATTGCGCGTGAAATT
GTTCTCGGTACCGGAATGAATGTGCATACCGATGCTTACAGCGTCAGCCGCGCTTGCGCTACCAGTTTCCAAGCGGTTGC
AAACGTCGCAGAAAGCCTGATGGCGGGAACTATCCGAGCGGGGATTGCCGGTGGGGCAGATTCCTCTTCCGTATTGCCAA
TTGGCGTCAGTAAAAAACTGGCGTACGTGCTGGTTGATGTCAACAAAGCTCGAACCATGAGCCAGCGCCTGAAACTCTTC
TCTCGCCTGCGTTTGCGCGACTTAATGCCCGTACCGCCTGCGGTAGCAGAATATTCTACTGGCTTGCGGATGGGCGACAC
CGCCGAACAAATGGCGAAAACCTACGGCATCACCCGAGAACAGCAAGATGCATTAGCGCACCGTTCGCATCAGCGTGCCG
CTCAGGCATGGTCAGACGGAAAACTCAAAGAAGAGGTGATGACTGCCTTTATCCCTCCTTATAAACAACCGCTTGTCGAA
GACAACAATATTCGCGGTAATTCCTCGCTTGCCGATTACGCAAAGCTGCGCCCGGCGTTTGATCGCAAACACGGAACGGT
AACGGCGGCAAACAGTACGCCGCTGACCGATGGCGCGGCAGCGGTGATCCTGATGACTGAATCCCGGGCGAAAGAATTAG
GGCTGGTGCCGCTGGGGTATCTGCGCAGCTACGCATTTACTGCGATTGATGTCTGGCAGGACATGTTGCTCGGTCCAGCC
TGGTCAACACCGCTGGCGCTGGAACGTGCCGGTTTGACGATGAGCGAACTGACATTGATCGATATGCACGAAGCCTTTGC
AGCTCAGACACTGGCGAATATTCAGTTGCTGGGTAGTGAACGTTTTGCTCGTGAAGTACTGGGGCGTGCACATGCCACTG
GCGAAGTGGACGATAGCAAATTTAACGTGCTTGGCGGTTCGATTGCTTACGGACATCCCTTCGCGGCGACCGGCGCGCGG
ATGATTACCCAGACATTGCATGAACTTCGCCGTCGCGGCGGTGGATTTGGTTTAGTTACCGCCTGTGCTGCCGGTGGGCT
TGGCGCGGCAATGGTTCTGGAGGCGGAATAA

Upstream 100 bases:

>100_bases
GCTCCCACTTTCCTTTTTCTCCGCTTGGCTAAATGTAAAAAAATGGTTAAGACTATGATCAGGTCAGACCACTTTATTTA
TTTTTTTACAGGGGAGTGTT

Downstream 100 bases:

>100_bases
TGGAAATGGCATCAGCGTTTACTCTTAATGTTCGTCTGGACAACATTGCTATCATCACCATCGACGTACCGGATGAGAAA
ATGAATACCCTGAAGGCGGA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREI
VLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPA
WSTPLALERAGLTMSELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGAR
MITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE

Sequences:

>Translated_436_residues
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREI
VLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPA
WSTPLALERAGLTMSELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGAR
MITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE
>Mature_435_residues
GQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMVVGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIV
LGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLFS
RLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVED
NNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAW
STPLALERAGLTMSELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARM
ITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=421, Percent_Identity=41.5676959619952, Blast_Score=335, Evalue=4e-92,
Organism=Homo sapiens, GI4557237, Length=423, Percent_Identity=30.0236406619385, Blast_Score=205, Evalue=7e-53,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=32.3255813953488, Blast_Score=193, Evalue=3e-49,
Organism=Homo sapiens, GI167614485, Length=424, Percent_Identity=29.9528301886792, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI148539872, Length=428, Percent_Identity=29.9065420560748, Blast_Score=180, Evalue=3e-45,
Organism=Homo sapiens, GI194353979, Length=106, Percent_Identity=38.6792452830189, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1788683, Length=436, Percent_Identity=99.0825688073395, Blast_Score=878, Evalue=0.0,
Organism=Escherichia coli, GI87082165, Length=422, Percent_Identity=32.7014218009479, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1788554, Length=423, Percent_Identity=35.2245862884161, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI48994986, Length=428, Percent_Identity=34.5794392523364, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1787663, Length=426, Percent_Identity=32.6291079812207, Blast_Score=181, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=38.2978723404255, Blast_Score=295, Evalue=3e-80,
Organism=Caenorhabditis elegans, GI133906874, Length=426, Percent_Identity=30.7511737089202, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI25147385, Length=420, Percent_Identity=28.3333333333333, Blast_Score=164, Evalue=6e-41,
Organism=Caenorhabditis elegans, GI17535921, Length=425, Percent_Identity=28.7058823529412, Blast_Score=156, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17535917, Length=427, Percent_Identity=23.1850117096019, Blast_Score=85, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6325229, Length=434, Percent_Identity=29.9539170506912, Blast_Score=172, Evalue=8e-44,
Organism=Saccharomyces cerevisiae, GI6322031, Length=440, Percent_Identity=28.1818181818182, Blast_Score=158, Evalue=2e-39,
Organism=Drosophila melanogaster, GI17137578, Length=430, Percent_Identity=40.9302325581395, Blast_Score=314, Evalue=8e-86,
Organism=Drosophila melanogaster, GI24655093, Length=422, Percent_Identity=29.6208530805687, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24640423, Length=426, Percent_Identity=28.169014084507, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17648125, Length=430, Percent_Identity=29.0697674418605, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 46610; Mature: 46479

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMVVGELLARSEIPAEVIE
CCCCCCHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST
CCCCCCCCCCEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
CCCCCCCCCHHHHHHHCHHHHCCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCCCCHHH
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSELTLIDMHEAFAAQTLANIQLLGSE
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHH
RFAREVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
HHHHHHHHHHCCCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMVLEAE
HHHCCCCCEEEEEECC
>Mature Secondary Structure 
GQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMVVGELLARSEIPAEVIE
CCCCCHHCCCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHH
QLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATSFQAVANVAESLMAGTIRA
HHHHHHHHCCCCCCCHHHHHHHCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLFSRLRLRDLMPVPPAVAEYST
CCCCCCCCCCEEEECCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHC
GLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWSDGKLKEEVMTAFIPPYKQPLVE
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCC
DNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLTDGAAAVILMTESRAKELGLVPLGY
CCCCCCCCCHHHHHHHCHHHHCCCCEEEECCCCCCCCCCEEEEEEECCHHHHCCCCCHHH
LRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTMSELTLIDMHEAFAAQTLANIQLLGSE
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHH
RFAREVLGRAHATGEVDDSKFNVLGGSIAYGHPFAATGARMITQTLHELRRRGGGFGLVT
HHHHHHHHHHCCCCCCCCCCEEEECCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHH
ACAAGGLGAAMVLEAE
HHHCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA