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The map label for this gene is hisM

Identifier: 30063733

GI number: 30063733

Start: 2412086

End: 2412802

Strand: Reverse

Name: hisM

Synonym: S2518

Alternate gene names: 30063733

Gene position: 2412802-2412086 (Counterclockwise)

Preceding gene: 30063734

Following gene: 30063732

Centisome position: 52.46

GC content: 50.07

Gene sequence:

>717_bases
GTGATCGAAATCTTACATGAATACTGGAAACCGCTGCTGTGGACCGACGGTTATCGCTTTACTGGTGTGGCGATCACTCT
GTGGCTGCTTATTTTGTCGGTAGTGATAGGCGGAGTCCTGGCGCTGTTTCTGGCGATTGGTCGTGTCTCCAGTAATAAAT
ACATCCAGTTTCCAATCTGGTTATTTACCTATATTTTTCGCGGTACGCCGCTGTATGTTCAGTTGCTGGTGTTCTATTCC
GGCATGTACACGCTTGAGATTGTTAAGGGAACCGAATTCCTTAACGCTTTCTTCCGCAGTGGCCTGAACTGTACCGTGCT
GGCGCTGACGCTTAACACCTGCGCTTACACTACCGAGATTTTTGCTGGGGCAATCCGTTCGGTTCCGCATGGGGAAATTG
AAGCCGCCAGAGCCTATGGCTTCTCGACCTTTAAAATGTATCGCTGCATTATTTTGCCTTCTGCGCTGCGTATTGCGTTA
CCGGCATACAGCAACGAAGTGATCCTGATGCTGCACTCTACTGCGCTGGCGTTTACGGCCACTGTGCCGGATCTGCTGAA
AATAGCCCGCGATATTAACGCCGCCACGTATCAACCTTTTACCGCCTTCGGCATTGCCGCGGTGCTCTATTTAATCATCT
CTTATGTCCTGATCAGCCTCTTTCGCAGAGCGGAAAAACGCTGGTTGCAGCATGTGAAACCTTCTTCAACGCACTGA

Upstream 100 bases:

>100_bases
TCGTCTGTGGCGTGATTTACCTGGTTTTCACCACCGTTTCCAATGGTGTGCTGCTGTTCCTTGAGCGCCGCTACTCCGTG
GGTGTGAAGAGGGCTGACCT

Downstream 100 bases:

>100_bases
GAATACGATGTCCGAGAATAAATTAAACGTTATCGATTTGCACAAACGCTATGGCGAACACGAAGTGCTGAAAGGGGTCT
CACTGCAAGCGAATGCCGGA

Product: histidine transport, membrane protein M

Products: ADP; phosphate; L-histidine [Cytoplasm]; ADP; L-ornithine [Cytoplasm]; L-arginine [Cytoplasm]; L-lysine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFLAIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYS
GMYTLEIVKGTEFLNAFFRSGLNCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLISLFRRAEKRWLQHVKPSSTH

Sequences:

>Translated_238_residues
MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFLAIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYS
GMYTLEIVKGTEFLNAFFRSGLNCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLISLFRRAEKRWLQHVKPSSTH
>Mature_238_residues
MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFLAIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYS
GMYTLEIVKGTEFLNAFFRSGLNCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLISLFRRAEKRWLQHVKPSSTH

Specific function: Part of the binding-protein-dependent transport system for histidine; probably responsible for the translocation of the substrate across the membrane

COG id: COG4160

COG function: function code E; ABC-type arginine/histidine transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1788645, Length=238, Percent_Identity=100, Blast_Score=482, Evalue=1e-137,
Organism=Escherichia coli, GI1787086, Length=208, Percent_Identity=31.7307692307692, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1788226, Length=213, Percent_Identity=34.2723004694836, Blast_Score=100, Evalue=7e-23,
Organism=Escherichia coli, GI1788646, Length=225, Percent_Identity=32, Blast_Score=97, Evalue=8e-22,
Organism=Escherichia coli, GI1787030, Length=206, Percent_Identity=30.5825242718447, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787087, Length=221, Percent_Identity=26.6968325791855, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1786874, Length=217, Percent_Identity=27.6497695852535, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082239, Length=212, Percent_Identity=27.8301886792453, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786873, Length=226, Percent_Identity=27.4336283185841, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI48994930, Length=200, Percent_Identity=27.5, Blast_Score=64, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISM_ECO57 (P0AEU5)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   G91027
- RefSeq:   NP_288881.1
- RefSeq:   NP_311218.1
- ProteinModelPortal:   P0AEU5
- SMR:   P0AEU5
- EnsemblBacteria:   EBESCT00000027070
- EnsemblBacteria:   EBESCT00000057970
- GeneID:   916899
- GeneID:   957390
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3569
- KEGG:   ecs:ECs3191
- GeneTree:   EBGT00050000008884
- HOGENOM:   HBG704821
- OMA:   QPFYAFG
- ProtClustDB:   PRK15069
- BioCyc:   ECOL83334:ECS3191-MONOMER
- InterPro:   IPR010065
- InterPro:   IPR000515
- TIGRFAMs:   TIGR01726

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 26870; Mature: 26870

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1337ee6c)-; HASH(0xf1294fc)-; HASH(0x1314d4c4)-; HASH(0x13217e9c)-; HASH(0x13af88bc)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFLAIGRVSSNKYIQFPIW
CHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHH
LFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGLNCTVLALTLNTCAYTTEI
HHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHH
FAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIALPAYSNEVILMLHSTALAFTA
HHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHH
TVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLISLFRRAEKRWLQHVKPSSTH
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
>Mature Secondary Structure
MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFLAIGRVSSNKYIQFPIW
CHHHHHHHCCHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHH
LFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGLNCTVLALTLNTCAYTTEI
HHHHHHCCCHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHH
FAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIALPAYSNEVILMLHSTALAFTA
HHHHHHCCCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHHHH
TVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLISLFRRAEKRWLQHVKPSSTH
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-histidine [Periplasm]; H2O; ATP; L-ornithine [Periplasm]; L-arginine [Periplasm]; L-lysine [Periplasm] [C]

Specific reaction: ATP + L-histidine [Periplasm] + H2O = ADP + phosphate + L-histidine [Cytoplasm] ATP + L-ornithine [Periplasm] + H2O = ADP + phosphate + L-ornithine [Cytoplasm] ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] ATP + L-lysine [P

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796