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The map label for this gene is menE

Identifier: 30063691

GI number: 30063691

Start: 2365835

End: 2367190

Strand: Reverse

Name: menE

Synonym: S2473

Alternate gene names: 30063691

Gene position: 2367190-2365835 (Counterclockwise)

Preceding gene: 30063692

Following gene: 30063683

Centisome position: 51.47

GC content: 56.19

Gene sequence:

>1356_bases
ATGATCTTCTCTGACTGGCCGTGGCGTCACTGGCGGCAAGTGCGGGGAGAAGCCATCGCCTTACGTCTTAATGACGAGCA
ACTCAACTGGCGCGAGCTTTGTGCTCGCGTCGATGAGTTAGCTACGGGCTATGCGGTGCAGGGGGTGGTTGAGGGCAGCG
GCGTGATGTTGCGGGCGTGGAATACGCCGCAAACGCTGCTCGCCTGGCTGGCGTTACTGCAATGCGGGGCGCGGGTGTTG
CCCGTGAACCCTCAGCTGCCGCAACCGTTGCTTGAAGAATTGCTGCCCAATCTGACGCTGCAATTTGCGCTGGTGCCGGA
TGGGGAAAACACGTTTCCGGCATTAACGTCGCTGCACATTCAGCTGGTTGAAGGCGTACATGCCGCTGCGTGGCAGCCGA
CGCGTCTGTGCTCAATGACGTTGACCTCGGGTTCTACCGGTTTGCCGAAAGCCGCTGTCCATACTTATCAGGCCCATCTT
GCCAGTGCGCAAGGTGTGTTATCGCTGATTCCGTTTGGCGATCACGATGACTGGTTGCTCTCTTTACCGCTGTTTCACGT
CTCCGGTCAGGGAATTATGTGGCGCTGGTTATACGCTGGTGCGCGGATGACGGTGCGTGATAAACAGCCATTGGAGCAAA
TGCTGGCAGGCTGTACTCACGCTTCACTGGTGCCAACACAACTCTGGCGTTTGCTGGTTAACCGCAGTTCCGTTTCCCTG
AAAGCGGTGTTACTTGGCGGCGCGGCTATCCCGGTCGAGTTGACGGAACAGGCGCGCGAGCAGGGGATTCGTTGCTTTTG
CGGCTATGGTCTGACCGAGTTTGCCTCCACGGTATGTGCGAAAGAAGCCGACGGCCTGGCAGACGTTGGTTTGCCGCTGC
CGGGTCGGGAAGTGAAAATCGTTAATAATGAAGTGTGGCTGCGGGCTGCCAGTATGGCAGAAGGTTACTGGCGTAACGGG
CAACTGGTTTCACTGGTTAATGACGAAGGCTGGTACGCTACGCGCGATCGCGGTGAGATGCATAATGGCAAGCTGACCAT
TGTCGGACGTTTAGACAATCTGTTCTTCAGTGGCGGTGAGGGTATTCAGCCGGAAGAAGTCGAGCGTGTTATTGCTGCAC
ATCCTGCGGTTTTGCAGGTGTTTATCGTTTCCGTTGCCGACAAGGAGTTTGGTCATCGGCCGGTAGCGGTGGTGGAGTAT
GACCAGCAAACCGTTGATCTTGGCGAATGGGTGAAAGATAAGCTGGCTCGTTTTCAACAACCGGTGCGCTGGCTGACTTT
ACCGCCGGAGCTGAAAAACGGCGGTATTAAAATTTCACGTCAGGCGCTAAAAGAGTGGGTGCAACGTCAGCAATAA

Upstream 100 bases:

>100_bases
CGGGACTGGACACGCTGGATCTGATGCAGGCGCAGCAGGTTCGTCGCTGGCCGGGGAGCCCACTGCCGTTAGTGGACGCT
GATGTACAGGAGCAGTTGTT

Downstream 100 bases:

>100_bases
TTAACCCTCCGTTTCACTTCTCATTTCTGCCATTCCATTGCCGTTGATAAAGAGTTTATCTGTTAATCTGTAAATTGATG
TGAAAACTCTTAGCAAACAG

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: o-succinylbenzoyl-CoA synthetase; OSB-CoA synthetase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGYAVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNG
QLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGHRPVAVVEY
DQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ

Sequences:

>Translated_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGYAVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNG
QLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGHRPVAVVEY
DQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ
>Mature_451_residues
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGYAVQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVL
PVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNG
QLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGHRPVAVVEY
DQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQQ

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Homo sapiens, GI122937307, Length=421, Percent_Identity=24.9406175771971, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1788595, Length=451, Percent_Identity=97.3392461197339, Blast_Score=859, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=350, Percent_Identity=25.1428571428571, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI221142682, Length=496, Percent_Identity=22.7822580645161, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1786810, Length=507, Percent_Identity=23.2741617357002, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788107, Length=352, Percent_Identity=26.4204545454545, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32563687, Length=461, Percent_Identity=25.5965292841649, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI17558820, Length=473, Percent_Identity=26.215644820296, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=499, Percent_Identity=24.8496993987976, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71994703, Length=342, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994694, Length=342, Percent_Identity=27.4853801169591, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71994690, Length=342, Percent_Identity=27.4853801169591, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25141359, Length=400, Percent_Identity=24.25, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17557194, Length=492, Percent_Identity=22.9674796747967, Blast_Score=79, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17538037, Length=495, Percent_Identity=22.4242424242424, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560308, Length=295, Percent_Identity=25.7627118644068, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24581924, Length=531, Percent_Identity=22.4105461393597, Blast_Score=86, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 50141; Mature: 50141

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGYAVQGVVEGSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCEEEEEC
NTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHI
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHH
QLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAQGVLSLIPFGDHDDWLL
HHHHCHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGLPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
VNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGE
ECCCEEEEEHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCCCEEEEEEEECCCEECCCC
GIQPEEVERVIAAHPAVLQVFIVSVADKEFGHRPVAVVEYDQQTVDLGEWVKDKLARFQQ
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ
CCEEEECCHHHCCCCEEEEHHHHHHHHHHCC
>Mature Secondary Structure
MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGYAVQGVVEGSGVMLRAW
CCCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEEECCCEEEEEC
NTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHI
CCHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHH
QLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAQGVLSLIPFGDHDDWLL
HHHHCHHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCCCEEE
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
EEEEEEECCCCEEEEEEECCCEEEECCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEE
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGLPLPGREVKI
EEEEECCCCCCHHHHHHHHHCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
VNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGE
ECCCEEEEEHHHHCCCCCCCEEEEEEECCCCEEECCCCCCCCCEEEEEEEECCCEECCCC
GIQPEEVERVIAAHPAVLQVFIVSVADKEFGHRPVAVVEYDQQTVDLGEWVKDKLARFQQ
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHC
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ
CCEEEECCHHHCCCCEEEEHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626063; 9205837; 9278503 [H]