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The map label for this gene is yeiT [H]

Identifier: 30063590

GI number: 30063590

Start: 2236278

End: 2237516

Strand: Direct

Name: yeiT [H]

Synonym: S2360

Alternate gene names: 30063590

Gene position: 2236278-2237516 (Clockwise)

Preceding gene: 30063589

Following gene: 30063599

Centisome position: 48.62

GC content: 51.33

Gene sequence:

>1239_bases
ATGCCGCAACAAAATTATCTGGATGAACTCACTCCGGCTTTTACGCCGTTACTGGCGATTAAAGAAGCCTCTCGCTGTTT
ATTATGTCACGACGCTCCCTGTAGTCAGGCTTGCCCGGCACAGACCGATCCGGGGAAATTTATTCGCTCAATCTACTTTC
GTAATTTTAAAGGCGCTGCCGAGACAATTCGCGAAAATAATGCCCTCGGTGCCGTTTGTGCCAGAGTGTGCCCGACGGAG
AAATTATGCCAAAGCGGTTGTACCCGTGCCGGTGTTGATGCTCCCATTGATATCGGCCGCTTACAGCGTTTTGTTACTGA
TTTTGAACAACAAACCGGGATGGAAATTTATCAGCCCGGTACTAAAACGCTCGGCAAAGTCGCGATTATTGGCGCAGGTC
CTGCCGGATTGCAGGCCAGTGTGACACTGACAAACCAGGGTTATGACGTCACGATTTATGAGAAAGAAGCGCACCCCGGT
GGCTGGTTGCGTAACGGTATTCCGCAATTCCGGTTACCGCAATCAGTGCTGGATGCAGAGATCGCCCGTATTGAAAAAAT
GGGCGTGACCATTAAGTGCAATAACGAAATCGGTAAAACACTCACCCTGGAGCAACTGAAAGCGGAAAACCGCGCGGTAC
TGGTCACCGTGGGGTTATCAAGCGGTTCCGGGCTACCGCTGTTTGAGCATAGCGACGTTGAGATTGCCGTCGACTTCCTG
CAACGTGTACGACAGGCGCAAGGCGATATCAGCATTCCACAAAGCGCATTAATTATCGGCGGCGGTGATGTCGCGATGGA
CGTAGCCAGCACGCTGAAAGTTCTCGGCTGTCAGGCGGTAACTTGCGTAGCACGTGAAGAGTTAGATGAGTTTCCGGCAA
GCGAAAAAGAGTTTGCCAGCGCCCGGGAGCTGGGCGTTTCGATCATCGATGGATTCACGCCAGTAGCCGTGGAAGGCAAT
AAAGTCACGTTTAAGCATGTACGGTTATCAGGCGAACTGACGATGGCGGCAGATAAAATTATTCTCGCCGTCGGTCAGCA
TGCCAGACTGGATGCCTTTGCGGAGTTAGAGCAGCAGCGTAACACCATCAAAACACAAAATTATCAGATCCGCGATCCGC
AAGTCTTTGCTGCTGGCGATATTGTTGAGGGTGACAAAACCGTGGTCTATGCCGTGAAAACCGGGAAAGAAGCCGCCGAG
GCGATTCATCACTATTTAGAGGGAGCTTGCTCATGTTAA

Upstream 100 bases:

>100_bases
CTTTCACAGCTTAAATTTCCCCACGCGCATAGCCTTAATATCAGCACGCAATATTTATTTAGCGTTCGCCATAATTACTC
ATAGCATTAAGGAAGATCAC

Downstream 100 bases:

>100_bases
CGAAAGATCTTTCGATTACTTTTTGCGGCGTGAACTTTCCCAACCCGTTCTGCCTCTCTTCTTCGCCGGTAGGCAACTGC
TATGAGATGTGTGCCAAAGC

Product: putative oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 412; Mature: 411

Protein sequence:

>412_residues
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
QRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGN
KVTFKHVRLSGELTMAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAVKTGKEAAE
AIHHYLEGACSC

Sequences:

>Translated_412_residues
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTE
KLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
QRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGN
KVTFKHVRLSGELTMAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAVKTGKEAAE
AIHHYLEGACSC
>Mature_411_residues
PQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEK
LCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPGG
WLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFLQ
RVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNK
VTFKHVRLSGELTMAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEA
IHHYLEGACSC

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=437, Percent_Identity=31.350114416476, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI237757300, Length=90, Percent_Identity=48.8888888888889, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1788468, Length=412, Percent_Identity=98.3009708737864, Blast_Score=833, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=449, Percent_Identity=31.8485523385301, Blast_Score=194, Evalue=8e-51,
Organism=Escherichia coli, GI1789606, Length=442, Percent_Identity=31.2217194570136, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI87082180, Length=449, Percent_Identity=28.9532293986637, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1789243, Length=398, Percent_Identity=29.3969849246231, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI71984108, Length=441, Percent_Identity=33.3333333333333, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17570289, Length=452, Percent_Identity=27.6548672566372, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6320030, Length=461, Percent_Identity=25.5965292841649, Blast_Score=126, Evalue=7e-30,
Organism=Drosophila melanogaster, GI24640763, Length=434, Percent_Identity=31.7972350230415, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI18858217, Length=434, Percent_Identity=31.7972350230415, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24665547, Length=444, Percent_Identity=27.7027027027027, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI24665543, Length=444, Percent_Identity=27.7027027027027, Blast_Score=131, Evalue=7e-31,
Organism=Drosophila melanogaster, GI28574881, Length=446, Percent_Identity=28.0269058295964, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24665539, Length=446, Percent_Identity=28.0269058295964, Blast_Score=131, Evalue=8e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: NA

Molecular weight: Translated: 44409; Mature: 44278

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
TKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPGGWLRNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHH
QRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFAS
HHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELTMAADKIILAVGQHARLDAFAELEQQR
HHHHCHHHHCCCCCEEEECCEEEEEEEEECCCEEEHHCEEEEEECCCCCHHHHHHHHHHH
NTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEAIHHYLEGACSC
HHHCCCCEEECCCCEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDPGKFIRSIYFRNFKGAA
CCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHHH
ETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQRFVTDFEQQTGMEIYQPG
HHHHCCCCHHHHHHHHCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCC
TKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPGGWLRNGIPQFRLPQSVLDAE
CCCCCCEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCHHHCCCCCCCCCHHHHHHH
IARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVGLSSGSGLPLFEHSDVEIAVDFL
HHHHHHHCEEEEECCCCCCEEEHHHHHCCCCEEEEEEEECCCCCCCEEECCCHHHHHHHH
QRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLGCQAVTCVAREELDEFPASEKEFAS
HHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELTMAADKIILAVGQHARLDAFAELEQQR
HHHHCHHHHCCCCCEEEECCEEEEEEEEECCCEEEHHCEEEEEECCCCCHHHHHHHHHHH
NTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAVKTGKEAAEAIHHYLEGACSC
HHHCCCCEEECCCCEEEECCEECCCCEEEEEEECCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]