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The map label for this gene is intB [H]

Identifier: 30063434

GI number: 30063434

Start: 2043593

End: 2044342

Strand: Direct

Name: intB [H]

Synonym: S2152

Alternate gene names: 30063434

Gene position: 2043593-2044342 (Clockwise)

Preceding gene: 30063433

Following gene: 30063436

Centisome position: 44.43

GC content: 52.93

Gene sequence:

>750_bases
ATGCGCCATGCGGTCCATCAGGAGTTAATCGATACGAACCCTGCAGCAAACCTTGGCGGCGTGACCACACCTCCTGTCAG
ACGGCACTATCCTGCCCTGCCGCTGGAGCGGCTGCCTGAACTGCTTGAACGTATTGGGGCATATCATCAGGGCCGTGAAC
TGACCCGGCATGCCGTTCTGCTGATGCTGCATGTGTTCATTCGCTCCAGTGAACTGCGTTTCGCCCGCTGGTCAGAGATT
GATTTCACAAACCGAGTCTGGACGATACCCGCGACGCGAGAACCCATTATTGGCGTGCGTTATTCCGGCCGCGGGGCAAA
AATGCGAATGCCGCATATCGTCCCCCTCTCAGAACAGTCCATCGCCATTCTGAAACAGATTAAGGATATCACCGGTAATA
ATGAACTGATCTTCCCCGGCGACCATAACCCGTATAAGCCAATGTGTGAAAACACGGTCAATAAGGCACTGCGGGTGATG
GGTTACGACACGAAAAAGGATATCTGCGGTCACGGCTTCCGGGCAATGGCATGCAGTGCGCTGATGGAATCGGGTTTATG
GGCAAAGGACGCAGTAGAACGCCAGATGAGTCATCAGGAGCGCAATACCGTGCGCATGGCTTATATTCATAAGGCAGAGC
ACCTGGAAGCCCGCAAAGCGATGATGCAGTGGTGGTCGGATTATCTGGAAGCATGCCGAGAATCTTATGCACCGCCTTAT
ACAATTGGTAAAAATAAGTTTATCCCATAG

Upstream 100 bases:

>100_bases
AGAACTTAAACCCCGTCATTTCATTGACCTGCTGAAAGGGATCGAGGAAAAAGGTCTGCTGGAGGTTGCGTCCCGCACAC
GGCAGCACCTGAGTAACATA

Downstream 100 bases:

>100_bases
TATACTAACGAATCCCACACTCTGGCAAATTACCACCACCTCTACAGGAGGTGGTTCTATATAGACTTTTATCAACTTCA
TGACGAAAAACACCTATTTG

Product: putative integrase

Products: NA

Alternate protein names: Int(P4) [H]

Number of amino acids: Translated: 249; Mature: 249

Protein sequence:

>249_residues
MRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGAYHQGRELTRHAVLLMLHVFIRSSELRFARWSEI
DFTNRVWTIPATREPIIGVRYSGRGAKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVM
GYDTKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAMMQWWSDYLEACRESYAPPY
TIGKNKFIP

Sequences:

>Translated_249_residues
MRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGAYHQGRELTRHAVLLMLHVFIRSSELRFARWSEI
DFTNRVWTIPATREPIIGVRYSGRGAKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVM
GYDTKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAMMQWWSDYLEACRESYAPPY
TIGKNKFIP
>Mature_249_residues
MRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGAYHQGRELTRHAVLLMLHVFIRSSELRFARWSEI
DFTNRVWTIPATREPIIGVRYSGRGAKMRMPHIVPLSEQSIAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVM
GYDTKKDICGHGFRAMACSALMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAMMQWWSDYLEACRESYAPPY
TIGKNKFIP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1788974, Length=232, Percent_Identity=34.9137931034483, Blast_Score=137, Evalue=5e-34,
Organism=Escherichia coli, GI1788690, Length=231, Percent_Identity=31.1688311688312, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI145693166, Length=237, Percent_Identity=31.6455696202532, Blast_Score=105, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109 [H]

Pfam domain/function: PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 28655; Mature: 28655

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGAYHQGRELTRHAVL
CCHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
LMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRGAKMRMPHIVPLSEQS
HHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCEECCCCCHH
IAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYDTKKDICGHGFRAMACSA
HHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHH
LMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAMMQWWSDYLEACRESYAPPY
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
TIGKNKFIP
ECCCCCCCC
>Mature Secondary Structure
MRHAVHQELIDTNPAANLGGVTTPPVRRHYPALPLERLPELLERIGAYHQGRELTRHAVL
CCHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
LMLHVFIRSSELRFARWSEIDFTNRVWTIPATREPIIGVRYSGRGAKMRMPHIVPLSEQS
HHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCCCCCEECCCCCHH
IAILKQIKDITGNNELIFPGDHNPYKPMCENTVNKALRVMGYDTKKDICGHGFRAMACSA
HHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHHHHH
LMESGLWAKDAVERQMSHQERNTVRMAYIHKAEHLEARKAMMQWWSDYLEACRESYAPPY
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
TIGKNKFIP
ECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503 [H]