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The map label for this gene is amyA

Identifier: 30063369

GI number: 30063369

Start: 1978958

End: 1980445

Strand: Direct

Name: amyA

Synonym: S2066

Alternate gene names: 30063369

Gene position: 1978958-1980445 (Clockwise)

Preceding gene: 30063368

Following gene: 30063371

Centisome position: 43.03

GC content: 49.8

Gene sequence:

>1488_bases
ATGCGTAATCCCACGCTGTTACAATGTTTTCACTGGTATTACCCGGAAGGCGGTAAGCTCTGGCCTGAACTGGCCGAGCG
CGCAGACGGTTTTAATGATATTGGTATCAATATGGTCTGGTTGCCGCCCGCCTATAAAGGCGCATCGGGCGGCTATTCGG
TCGGCTACGACTCCTATGATTTATTTGATCTAGGTGAGTTTGATCAGAAAGGCAGCATCCCTACTAAATATGGTGATAAA
GCACAACTGCTGGCCGCCATTGACGCCCTGAAACGTAATGACATTGCGGTGCTGCTGGACGTGGTAGTCAACCACAAAAT
GGGCGCGGATGAAAAAGAAGCTATTCGCGTGCAGCGTGTAAATGCTGATGACCGTACGCAAATTGACGAAGAAATCATTG
AGTGTGAAGGCTGGACGCGTTACACCTTCCCCGCTCGTGCCGGGCAATACTCGCAGTTTATCTGGGATTTCAAATGTTTT
AGCGGTATCGACCATATCGAACACCCTGATGAAGATGGCATTTTTAAAATTGTTAACGACTATACCGGAGAAGGCTGGAA
CGATCAGGTTGATGATGAACTCGGTAATTTCGATTATCTGATGGGTGAGAATATCGATTTTCGCAATCATGCCGTGACGG
AAGAGATTAAATACTGGGCGCGCTGGGTGATGGAACAAACGCAATGCGACGGCTTTCGTCTTGATGCGGTCAAACATATT
CCGGCATGGTTTTATAAAGAGTGGATCGAACACGTACAGGAAGTTGCGCCAAAGCCGCTGTTTATTGTGGCTGAATGCTG
GTCGCATGAAGTCGATAAACTGCAAACGTATATTGATCAGGTGGAAGGCAAAACCATGCTGTTTGATGCGCCGCTGCAGA
TGAAATTCCATGAAGCATCGCGCATGGGTCGCGACTACGACATGACGCAGATCTTCACTGGGACGCTGGTGGAAGCCGAT
CCTTTTCACGCCGTAACCCTCGTTGCCAACCACGACACCCAACCGTTGCAAGCCCTCGAAGCACCGGTCGAACCGTGGTT
TAAACCGCTGGCGTATGCCTTAATTTTGTTGCGGGAAAATGGCGTTCCTTCGGTGTTCTATCCGGACCTCTACGGTGCAC
ATTACGAAGATGTCGGTGGTGACGGGCAAACCTATCCGATAGATATGCCAATAATCGAACAGCTTGATGAGTTAATTCTC
GCCCGTCAGCGTTTCGCCCACGGTGTACAGACATTATTTTTCGACCATCCGAACTGCATTGCCTTTAGCCGCAGTGGCAC
CGACGAATATCCCGGCTGCGTGGTGGTCATGTCGAACGGAGATGATGGCGAAAAAACCATTCATCTGGGAGAGAATTACG
GCAATAAAACCTGGCGTGATTTTCTGGGGAACCGGCAAGAGAGCGTAGTGACAGACGAAAACGGCGAAGCGACCTTCTTT
TGCAACGGCGGCAGCGTCAGCGTGTGGGTTATCGAAGAGGTGATTTAA

Upstream 100 bases:

>100_bases
CTCCGCAGGATAACCTCTTTTGAATCTTAATGAGCCGATGGCTCGCGAATAATCCGATTACGGCTACGCTTCTAATATTC
CCCTTGAATGGAGTCGAAGA

Downstream 100 bases:

>100_bases
ATCCATCCCCGGCGGCAAGCCGGGGAGATTTCATTACGGCAGTGGCGTCGGTAACGGCGCTTTATCCAGCGCGGCGGCAC
ACTCTGCCGTCGGGCGATCC

Product: cytoplasmic alpha-amylase

Products: NA

Alternate protein names: 1,4-alpha-D-glucan glucanohydrolase [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPAYKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDK
AQLLAAIDALKRNDIAVLLDVVVNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTQCDGFRLDAVKHI
PAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQTYIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEAD
PFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPIIEQLDELIL
ARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQESVVTDENGEATFF
CNGGSVSVWVIEEVI

Sequences:

>Translated_495_residues
MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPAYKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDK
AQLLAAIDALKRNDIAVLLDVVVNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTQCDGFRLDAVKHI
PAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQTYIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEAD
PFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPIIEQLDELIL
ARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQESVVTDENGEATFF
CNGGSVSVWVIEEVI
>Mature_495_residues
MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPAYKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDK
AQLLAAIDALKRNDIAVLLDVVVNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTQCDGFRLDAVKHI
PAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQTYIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEAD
PFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPIIEQLDELIL
ARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQESVVTDENGEATFF
CNGGSVSVWVIEEVI

Specific function: Unknown

COG id: COG0366

COG function: function code G; Glycosidases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1788236, Length=495, Percent_Identity=99.1919191919192, Blast_Score=1021, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013776
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR006589
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00128 Alpha-amylase [H]

EC number: =3.2.1.1 [H]

Molecular weight: Translated: 56550; Mature: 56550

Theoretical pI: Translated: 4.26; Mature: 4.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPAYKGASGGYSVGYDSYD
CCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
LFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVVVNHKMGADEKEAIRVQRV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHCEEEEEC
NADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCFSGIDHIEHPDEDGIFKIVND
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEC
YTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTQCDGFRLDAVKHI
CCCCCCCCCHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
PAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQTYIDQVEGKTMLFDAPLQMKFHEAS
HHHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHCCCEEEEECCCCEEEHHHH
RMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLREN
HCCCCCCHHHHEECEEEECCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHEECC
GVPSVFYPDLYGAHYEDVGGDGQTYPIDMPIIEQLDELILARQRFAHGVQTLFFDHPNCI
CCCCEECCCCCCCCHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEE
AFSRSGTDEYPGCVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQESVVTDENGEATFF
EEECCCCCCCCCEEEEEECCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEECCCCCEEEE
CNGGSVSVWVIEEVI
ECCCCEEEEEEEECC
>Mature Secondary Structure
MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPAYKGASGGYSVGYDSYD
CCCCCEEEEEEEECCCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
LFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVVVNHKMGADEKEAIRVQRV
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHCEEEEEC
NADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCFSGIDHIEHPDEDGIFKIVND
CCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEC
YTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKYWARWVMEQTQCDGFRLDAVKHI
CCCCCCCCCHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHH
PAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQTYIDQVEGKTMLFDAPLQMKFHEAS
HHHHHHHHHHHHHHHCCCCEEEEEEHHCCHHHHHHHHHHHHCCCEEEEECCCCEEEHHHH
RMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQPLQALEAPVEPWFKPLAYALILLREN
HCCCCCCHHHHEECEEEECCCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHHHHEECC
GVPSVFYPDLYGAHYEDVGGDGQTYPIDMPIIEQLDELILARQRFAHGVQTLFFDHPNCI
CCCCEECCCCCCCCHHCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCEE
AFSRSGTDEYPGCVVVMSNGDDGEKTIHLGENYGNKTWRDFLGNRQESVVTDENGEATFF
EEECCCCCCCCCEEEEEECCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEECCCCCEEEE
CNGGSVSVWVIEEVI
ECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1400215; 9097040; 9278503; 1527488; 8371104 [H]