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The map label for this gene is flhB

Identifier: 30063328

GI number: 30063328

Start: 1936449

End: 1937642

Strand: Reverse

Name: flhB

Synonym: S2014

Alternate gene names: 30063328

Gene position: 1937642-1936449 (Counterclockwise)

Preceding gene: 30063330

Following gene: 30063327

Centisome position: 42.13

GC content: 55.11

Gene sequence:

>1194_bases
GTGTCTGACGAGAGCGACGACAAAACAGAAGCCCCCACACCTCACCGACTAGAAAAAGCGCGGGAAGAGGGGCAAATCCC
GCGTTCCCGTGAACTGACCTCACTGCTGATTTTGCTGGTGGGCGTTAGTGTTATCTGGTTTGGCGGTGTGTCGCTGGCCC
GTCGATTGTCGGGCATGCTCTCCGCTGGGCTGCATTTTGATCACAGTATTATCAAAGACCCGAATCTGATCCTCGGGCAA
ATTATTCTGCTGATCAGAGAAGCCATGCTGGCGCTGCTACCGCTGATTAGCGGCGTGGTGCTGGTGGCGCTCATTTCTCC
GGTTATGCTGGGGGGGCTGGTATTTAGCGGCAAATCCTTGCAGCCGAAGTTTTCCAAACTCAACCCGCTACCGGGCATTA
AACGGATATTCTCGGCACAGACTAGCGCGGAGTTGCTTAAGGCAATTTTGAAAACCATCCTGGTTGGCAGCGTGACGGGG
TTTTTTCTCTGGCATCACTGGCCGCAGATGATGCGCTTAATGGCCGAGTCACCGATTACCGCCATGGGTAATGCGATGGA
TTTGGTAGGGCTATGCGCACTGCTGGTGGTGCTTGGTGTCATTCCAATGGTGGGATTTGACGTCTTTTTCCAAATCTTCA
GCCACCTGAAAAAGCTGCGAATGTCGCGGCAGGATATTCGTGATGAGTTCAAACAAAGCGAAGGCGACCCCCATGTTAAA
GGACGGATCTGTCAGATGCAGCGAGCTGCCGCACGGCGTCGGATGATGGCCGATGTGCCGAAAGCGGATGTCATTGTCAA
TAACCCGACCCACTATTCGGTAGCGTTGCAGTATGACGAAAACAAAATGAGCGCACCGAAAGTGGTCGCTAAAGGTGCAG
GGCTGGTCGCGCTGCGCATTCGTGAAATTGGCGCTGAAAATAACGTCCCGACGCTTGAAGCGCCGCCGCTGGCGCGAGCG
CTGTATCGACATGCGGAGATTGGTCAACAAATCCCGGGTCAACTGTACGCCGCGGTGGCGGAAGTGCTGGCCTGGGTCTG
GCAACTGAAACGCTGGCGTCTGGCTGGTGGACAGCGCCCTGTACACCTACTCATCTTCCGGTGCCGGCAGCCCTGGATTT
TATTAACGAGAAACCGACCCATGAGTAATCTGGCCGCGATGCTGCGCCTGCCCGCAAACCTGAAATCGACATAA

Upstream 100 bases:

>100_bases
AAACGCCCTAAATCCCGCCTGTTTTGCCCCTTACTTAAACCATTGAACGCTTTGCGCTCTGGCATCATTTACGCTTAATA
CTCTTTCCAGGATTGGCGAC

Downstream 100 bases:

>100_bases
TGGCAGATCCTTGCCGGACCGATTTTGATCCTGTTGATCTTGTCGATGATGGTGCTGCCACTGCCCGCATTCATACTCGA
CCTGTTGTTTACCTTCAATA

Product: flagellar biosynthesis protein FlhB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSVIWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQ
IILLIREAMLALLPLISGVVLVALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTG
FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQSEGDPHVK
GRICQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARA
LYRHAEIGQQIPGQLYAAVAEVLAWVWQLKRWRLAGGQRPVHLLIFRCRQPWILLTRNRPMSNLAAMLRLPANLKST

Sequences:

>Translated_397_residues
MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSVIWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQ
IILLIREAMLALLPLISGVVLVALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTG
FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQSEGDPHVK
GRICQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARA
LYRHAEIGQQIPGQLYAAVAEVLAWVWQLKRWRLAGGQRPVHLLIFRCRQPWILLTRNRPMSNLAAMLRLPANLKST
>Mature_396_residues
SDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSVIWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQI
ILLIREAMLALLPLISGVVLVALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTGF
FLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQSEGDPHVKG
RICQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARAL
YRHAEIGQQIPGQLYAAVAEVLAWVWQLKRWRLAGGQRPVHLLIFRCRQPWILLTRNRPMSNLAAMLRLPANLKST

Specific function: Required for formation of the rod structure in the basal body of the flagellar apparatus. Together with fliI and fliH, may constitute the export apparatus of flagellin [H]

COG id: COG1377

COG function: function code NU; Flagellar biosynthesis pathway, component FlhB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type III secretion exporter family [H]

Homologues:

Organism=Escherichia coli, GI1788188, Length=362, Percent_Identity=98.6187845303867, Blast_Score=719, Evalue=0.0,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006136
- InterPro:   IPR006135 [H]

Pfam domain/function: PF01312 Bac_export_2 [H]

EC number: NA

Molecular weight: Translated: 44045; Mature: 43914

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSVIWFGGVSLARRLSGML
CCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFSGKSL
HHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTGFFLWHHWPQMMRLMAESPIT
CCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQSEGDPHVK
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
GRICQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVAKGAGLVALRI
HHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEEECCCCCCCCHHHHCCCCEEEHHH
REIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQLKRWRLAGGQRP
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VHLLIFRCRQPWILLTRNRPMSNLAAMLRLPANLKST
HHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSVIWFGGVSLARRLSGML
CCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFSGKSL
HHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
QPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTGFFLWHHWPQMMRLMAESPIT
CCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQSEGDPHVK
HHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
GRICQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVAKGAGLVALRI
HHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEEEEEEECCCCCCCCHHHHCCCCEEEHHH
REIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQLKRWRLAGGQRP
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
VHLLIFRCRQPWILLTRNRPMSNLAAMLRLPANLKST
HHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]